Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie
Abstract Background In the United States and Canada, ca. one-half of native orchid species are now threatened with extinction. A number of these species are restricted to tallgrass prairies of central North America, such as the Eastern Prairie Fringed Orchid, Platanthera leucophaea (Nutt.) Lindl.—a...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-04-01
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| Series: | Botanical Studies |
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| Online Access: | http://link.springer.com/article/10.1186/s40529-020-00289-z |
| _version_ | 1818957651067797504 |
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| author | Hana L. Thixton Elizabeth J. Esselman Laura L. Corey Lawrence W. Zettler |
| author_facet | Hana L. Thixton Elizabeth J. Esselman Laura L. Corey Lawrence W. Zettler |
| author_sort | Hana L. Thixton |
| collection | DOAJ |
| description | Abstract Background In the United States and Canada, ca. one-half of native orchid species are now threatened with extinction. A number of these species are restricted to tallgrass prairies of central North America, such as the Eastern Prairie Fringed Orchid, Platanthera leucophaea (Nutt.) Lindl.—a U.S. Federally threatened species. Results We provide new records of fungi recovered from roots of P. leucophaea and five other orchid species inhabiting prairie sites in Illinois and neighboring states during a 10-year period (2008–2017). A total of 39 fungal endophytes were isolated from Cypripedium candidum (1), Platanthera lacera (1), P. leucophaea (32), P. peramoena (3), Spiranthes lacera (1), and S. magnicamporum (1), 31 (79%) of which were assignable to Ceratobasidium and the remainder to Tulasnella. These fungi were acquired from 16 different sites, 13 of which are new records including two new state records (Iowa, Wisconsin). Molecular analysis revealed that some Ceratobasidium strains were virtually identical despite being geographically isolated by > 300 km. Conclusions This study, encompassing a decade of work, confirms that Platanthera leucophaea is a mycorrhizal specialist with heavy reliance on Ceratobasidium with the tallgrass prairie ecosystem of North America. Our isolation of Ceratobasidium from P. leucophaea spanning additional sites suggests that the association is widespread. Such information should provide conservationists and land managers with more confidence in developing protocols that facilitate the long-term conservation of this prairie orchid. |
| first_indexed | 2024-12-20T11:13:14Z |
| format | Article |
| id | doaj.art-a338a01cb9ee4bab8fd5a59f2639c6a3 |
| institution | Directory Open Access Journal |
| issn | 1999-3110 |
| language | English |
| last_indexed | 2024-12-20T11:13:14Z |
| publishDate | 2020-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Botanical Studies |
| spelling | doaj.art-a338a01cb9ee4bab8fd5a59f2639c6a32022-12-21T19:42:41ZengSpringerOpenBotanical Studies1999-31102020-04-016111810.1186/s40529-020-00289-zFurther evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairieHana L. Thixton0Elizabeth J. Esselman1Laura L. Corey2Lawrence W. Zettler3Department of Biology, Southern Illinois University EdwardsvilleDepartment of Biology, Southern Illinois University EdwardsvilleDepartment of Biology, Illinois CollegeDepartment of Biology, Illinois CollegeAbstract Background In the United States and Canada, ca. one-half of native orchid species are now threatened with extinction. A number of these species are restricted to tallgrass prairies of central North America, such as the Eastern Prairie Fringed Orchid, Platanthera leucophaea (Nutt.) Lindl.—a U.S. Federally threatened species. Results We provide new records of fungi recovered from roots of P. leucophaea and five other orchid species inhabiting prairie sites in Illinois and neighboring states during a 10-year period (2008–2017). A total of 39 fungal endophytes were isolated from Cypripedium candidum (1), Platanthera lacera (1), P. leucophaea (32), P. peramoena (3), Spiranthes lacera (1), and S. magnicamporum (1), 31 (79%) of which were assignable to Ceratobasidium and the remainder to Tulasnella. These fungi were acquired from 16 different sites, 13 of which are new records including two new state records (Iowa, Wisconsin). Molecular analysis revealed that some Ceratobasidium strains were virtually identical despite being geographically isolated by > 300 km. Conclusions This study, encompassing a decade of work, confirms that Platanthera leucophaea is a mycorrhizal specialist with heavy reliance on Ceratobasidium with the tallgrass prairie ecosystem of North America. Our isolation of Ceratobasidium from P. leucophaea spanning additional sites suggests that the association is widespread. Such information should provide conservationists and land managers with more confidence in developing protocols that facilitate the long-term conservation of this prairie orchid.http://link.springer.com/article/10.1186/s40529-020-00289-zSpecificityConservationEndophytesMycorrhizal fungiTulasnella |
| spellingShingle | Hana L. Thixton Elizabeth J. Esselman Laura L. Corey Lawrence W. Zettler Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie Botanical Studies Specificity Conservation Endophytes Mycorrhizal fungi Tulasnella |
| title | Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie |
| title_full | Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie |
| title_fullStr | Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie |
| title_full_unstemmed | Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie |
| title_short | Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie |
| title_sort | further evidence of ceratobasidium d p rogers basidiomycota serving as the ubiquitous fungal associate of platanthera leucophaea orchidaceae in the north american tallgrass prairie |
| topic | Specificity Conservation Endophytes Mycorrhizal fungi Tulasnella |
| url | http://link.springer.com/article/10.1186/s40529-020-00289-z |
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