22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm
Starch, the main form of stored energy in plants, plays an important role in maize (<i>Zea mays</i> L.) kernel development. The <i>Shrunken-2</i> (<i>Sh2</i>) gene encodes the large subunit of the rate-limiting starch biosynthetic enzyme ADP-glucose pyrophosphoryl...
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MDPI AG
2020-04-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/5/624 |
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| author | Ada Menie Nelly Sandrine Hailiang Zhao Yao Qin Qin Sun Dianming Gong Zhenyuan Pan Fazhan Qiu |
| author_facet | Ada Menie Nelly Sandrine Hailiang Zhao Yao Qin Qin Sun Dianming Gong Zhenyuan Pan Fazhan Qiu |
| author_sort | Ada Menie Nelly Sandrine |
| collection | DOAJ |
| description | Starch, the main form of stored energy in plants, plays an important role in maize (<i>Zea mays</i> L.) kernel development. The <i>Shrunken-2</i> (<i>Sh2</i>) gene encodes the large subunit of the rate-limiting starch biosynthetic enzyme ADP-glucose pyrophosphorylase (AGPase). The <i>sh2</i> mutant exhibits impaired AGPase activity, resulting in the partial or complete loss of starch synthesis. Here, we investigated the transcriptional regulatory framework of <i>sh2</i> through transcriptome and co-expression network analysis using an F<sub>2</sub> population derived from the maize reference line B73 and sweet corn inbred line HZ508. We identified 5175 differentially expressed genes (DEGs), including 2878 upregulated and 2297 downregulated genes in <i>sh2</i> mutant lines. DEGs are associated with various biological processes including nutrient reservoir activity, transferase activity, catalytic activity, water deprivation and glycogen metabolism. At the genetic level, 2465 DEGs, including 357 transcription factors, were involved in transcription. In addition, the maize floury and opaque mutant genes <i>fl1</i>, <i>ndk2,</i> <i>o7</i> and <i>o2</i>, which regulate the biosynthesis of 22KD zein, were co-expressed with the differential expressed transcription factor genes, thus suggesting that zein content might be a key regulator coordinating the expression of genes determining starch accumulation in maize endosperm. |
| first_indexed | 2024-03-10T20:11:39Z |
| format | Article |
| id | doaj.art-a339afdbe77c458fbb9d627a00c164c6 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T20:11:39Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-a339afdbe77c458fbb9d627a00c164c62023-11-19T22:55:38ZengMDPI AGAgronomy2073-43952020-04-0110562410.3390/agronomy1005062422KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize EndospermAda Menie Nelly Sandrine0Hailiang Zhao1Yao Qin2Qin Sun3Dianming Gong4Zhenyuan Pan5Fazhan Qiu6National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaStarch, the main form of stored energy in plants, plays an important role in maize (<i>Zea mays</i> L.) kernel development. The <i>Shrunken-2</i> (<i>Sh2</i>) gene encodes the large subunit of the rate-limiting starch biosynthetic enzyme ADP-glucose pyrophosphorylase (AGPase). The <i>sh2</i> mutant exhibits impaired AGPase activity, resulting in the partial or complete loss of starch synthesis. Here, we investigated the transcriptional regulatory framework of <i>sh2</i> through transcriptome and co-expression network analysis using an F<sub>2</sub> population derived from the maize reference line B73 and sweet corn inbred line HZ508. We identified 5175 differentially expressed genes (DEGs), including 2878 upregulated and 2297 downregulated genes in <i>sh2</i> mutant lines. DEGs are associated with various biological processes including nutrient reservoir activity, transferase activity, catalytic activity, water deprivation and glycogen metabolism. At the genetic level, 2465 DEGs, including 357 transcription factors, were involved in transcription. In addition, the maize floury and opaque mutant genes <i>fl1</i>, <i>ndk2,</i> <i>o7</i> and <i>o2</i>, which regulate the biosynthesis of 22KD zein, were co-expressed with the differential expressed transcription factor genes, thus suggesting that zein content might be a key regulator coordinating the expression of genes determining starch accumulation in maize endosperm.https://www.mdpi.com/2073-4395/10/5/624endosperm developmentmaize<i>sh2</i>starch accumulationzein |
| spellingShingle | Ada Menie Nelly Sandrine Hailiang Zhao Yao Qin Qin Sun Dianming Gong Zhenyuan Pan Fazhan Qiu 22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm Agronomy endosperm development maize <i>sh2</i> starch accumulation zein |
| title | 22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm |
| title_full | 22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm |
| title_fullStr | 22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm |
| title_full_unstemmed | 22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm |
| title_short | 22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm |
| title_sort | 22kd zein content coordinates transcriptional activity during starch synthesis in maize endosperm |
| topic | endosperm development maize <i>sh2</i> starch accumulation zein |
| url | https://www.mdpi.com/2073-4395/10/5/624 |
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