Electrochemical Approach to Detection of Chlorophene in Water Catalyzed by a Laccase Modified Gold Electrode
Despite the increasing number of reports that relate antimicrobial chlorophene (CP) with health and environmental effects, few studies have addressed biosensing technologies to detect this threat. This work proposed an electrochemical approach for the detection of CP using laccase enzymes as an alte...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/9/4/82 |
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| author | Gabriela Elizabeth Quintanilla-Villanueva Donato Luna-Moreno Araceli Sánchez-Álvarez Juan Francisco Villarreal-Chiu José Manuel Rodríguez-Delgado Melissa Marlene Rodríguez-Delgado |
| author_facet | Gabriela Elizabeth Quintanilla-Villanueva Donato Luna-Moreno Araceli Sánchez-Álvarez Juan Francisco Villarreal-Chiu José Manuel Rodríguez-Delgado Melissa Marlene Rodríguez-Delgado |
| author_sort | Gabriela Elizabeth Quintanilla-Villanueva |
| collection | DOAJ |
| description | Despite the increasing number of reports that relate antimicrobial chlorophene (CP) with health and environmental effects, few studies have addressed biosensing technologies to detect this threat. This work proposed an electrochemical approach for the detection of CP using laccase enzymes as an alternative recognition element immobilized onto thin-film gold electrodes. The electrochemical parameters of the detection method, under controlled conditions, resulted in a limit of detection (0.14 ± 0.06 mg L<sup>−1</sup>) and quantification (0.48 ± 0.04 mg L<sup>−1</sup>) that agreed with concentrations of CP that already had been measured in natural water samples. Nevertheless, during the analysis of natural river water samples, the provided method suffered a drawback due to matrix effects reflected in the obtained recovery percentage, the value of which was 62.0 ± 2.4% compared to the 101.3 ± 3.5% obtained by the HPLC reference method. These detrimental effects were mainly attributed to organic matter, SO4-2, and Cl- present in river samples. |
| first_indexed | 2024-03-10T12:16:39Z |
| format | Article |
| id | doaj.art-a33bd9edb29447a3b4f63447c4af0aee |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T12:16:39Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-a33bd9edb29447a3b4f63447c4af0aee2023-11-21T15:48:25ZengMDPI AGChemosensors2227-90402021-04-01948210.3390/chemosensors9040082Electrochemical Approach to Detection of Chlorophene in Water Catalyzed by a Laccase Modified Gold ElectrodeGabriela Elizabeth Quintanilla-Villanueva0Donato Luna-Moreno1Araceli Sánchez-Álvarez2Juan Francisco Villarreal-Chiu3José Manuel Rodríguez-Delgado4Melissa Marlene Rodríguez-Delgado5Universidad Autónoma de Nuevo León, Facultad de Ciencias Químicas, Laboratorio de Biotecnología, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza 66455, Nuevo León, MexicoCentro de Investigaciones en Óptica AC, Div. de Fotónica, Loma del Bosque 115, Col. Lomas del Campestre, León 37150, Guanajuato, MexicoUniversidad Tecnológica de León, Electromecánica Industrial, Blvd. Universidad Tecnológica #225, Col. San Carlos, León 37670, Guanajuato, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Químicas, Laboratorio de Biotecnología, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza 66455, Nuevo León, MexicoTecnológico de Monterrey, School of Engineering and Sciences, Av. Eugenio Garza Sada Sur No. 2501, Col. Tecnológico, Monterrey 64849, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Químicas, Laboratorio de Biotecnología, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza 66455, Nuevo León, MexicoDespite the increasing number of reports that relate antimicrobial chlorophene (CP) with health and environmental effects, few studies have addressed biosensing technologies to detect this threat. This work proposed an electrochemical approach for the detection of CP using laccase enzymes as an alternative recognition element immobilized onto thin-film gold electrodes. The electrochemical parameters of the detection method, under controlled conditions, resulted in a limit of detection (0.14 ± 0.06 mg L<sup>−1</sup>) and quantification (0.48 ± 0.04 mg L<sup>−1</sup>) that agreed with concentrations of CP that already had been measured in natural water samples. Nevertheless, during the analysis of natural river water samples, the provided method suffered a drawback due to matrix effects reflected in the obtained recovery percentage, the value of which was 62.0 ± 2.4% compared to the 101.3 ± 3.5% obtained by the HPLC reference method. These detrimental effects were mainly attributed to organic matter, SO4-2, and Cl- present in river samples.https://www.mdpi.com/2227-9040/9/4/82electrochemical biosensorenzymelaccasechloropheneemerging pollutantwater |
| spellingShingle | Gabriela Elizabeth Quintanilla-Villanueva Donato Luna-Moreno Araceli Sánchez-Álvarez Juan Francisco Villarreal-Chiu José Manuel Rodríguez-Delgado Melissa Marlene Rodríguez-Delgado Electrochemical Approach to Detection of Chlorophene in Water Catalyzed by a Laccase Modified Gold Electrode Chemosensors electrochemical biosensor enzyme laccase chlorophene emerging pollutant water |
| title | Electrochemical Approach to Detection of Chlorophene in Water Catalyzed by a Laccase Modified Gold Electrode |
| title_full | Electrochemical Approach to Detection of Chlorophene in Water Catalyzed by a Laccase Modified Gold Electrode |
| title_fullStr | Electrochemical Approach to Detection of Chlorophene in Water Catalyzed by a Laccase Modified Gold Electrode |
| title_full_unstemmed | Electrochemical Approach to Detection of Chlorophene in Water Catalyzed by a Laccase Modified Gold Electrode |
| title_short | Electrochemical Approach to Detection of Chlorophene in Water Catalyzed by a Laccase Modified Gold Electrode |
| title_sort | electrochemical approach to detection of chlorophene in water catalyzed by a laccase modified gold electrode |
| topic | electrochemical biosensor enzyme laccase chlorophene emerging pollutant water |
| url | https://www.mdpi.com/2227-9040/9/4/82 |
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