Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila
During development, many epithelia are formed by a mesenchymal-epithelial transition (MET). Here, we examine the major stages and underlying mechanisms of MET during blood-brain barrier formation in Drosophila. We show that contact with the basal lamina is essential for the growth of the barrier-for...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2017-02-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/6/2/232 |
| _version_ | 1818677987189456896 |
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| author | Tina Schwabe Xiaoling Li Ulrike Gaul |
| author_facet | Tina Schwabe Xiaoling Li Ulrike Gaul |
| author_sort | Tina Schwabe |
| collection | DOAJ |
| description | During development, many epithelia are formed by a mesenchymal-epithelial transition (MET). Here, we examine the major stages and underlying mechanisms of MET during blood-brain barrier formation in Drosophila. We show that contact with the basal lamina is essential for the growth of the barrier-forming subperineurial glia (SPG). Septate junctions (SJs), which provide insulation of the paracellular space, are not required for MET, but are necessary for the establishment of polarized SPG membrane compartments. In vivo time-lapse imaging reveals that the Moody GPCR signaling pathway regulates SPG cell growth and shape, with different levels of signaling causing distinct phenotypes. Timely, well-coordinated SPG growth is essential for the uniform insertion of SJs and thus the insulating function of the barrier. To our knowledge, this is the first dynamic in vivo analysis of all stages in the formation of a secondary epithelium, and of the key role trimeric G protein signaling plays in this important morphogenetic process. |
| first_indexed | 2024-12-17T09:08:06Z |
| format | Article |
| id | doaj.art-a33e65807f4641659ff5a1720b12375c |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T09:08:06Z |
| publishDate | 2017-02-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-a33e65807f4641659ff5a1720b12375c2022-12-21T21:55:23ZengThe Company of BiologistsBiology Open2046-63902017-02-016223224310.1242/bio.020669020669Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in DrosophilaTina Schwabe0Xiaoling Li1Ulrike Gaul2 Department of Biochemistry, Gene Center, Center of Integrated Protein Science (CIPSM), University of Munich, Feodor-Lynen-Str. 25, Munich 81377, Germany Department of Biochemistry, Gene Center, Center of Integrated Protein Science (CIPSM), University of Munich, Feodor-Lynen-Str. 25, Munich 81377, Germany Department of Biochemistry, Gene Center, Center of Integrated Protein Science (CIPSM), University of Munich, Feodor-Lynen-Str. 25, Munich 81377, Germany During development, many epithelia are formed by a mesenchymal-epithelial transition (MET). Here, we examine the major stages and underlying mechanisms of MET during blood-brain barrier formation in Drosophila. We show that contact with the basal lamina is essential for the growth of the barrier-forming subperineurial glia (SPG). Septate junctions (SJs), which provide insulation of the paracellular space, are not required for MET, but are necessary for the establishment of polarized SPG membrane compartments. In vivo time-lapse imaging reveals that the Moody GPCR signaling pathway regulates SPG cell growth and shape, with different levels of signaling causing distinct phenotypes. Timely, well-coordinated SPG growth is essential for the uniform insertion of SJs and thus the insulating function of the barrier. To our knowledge, this is the first dynamic in vivo analysis of all stages in the formation of a secondary epithelium, and of the key role trimeric G protein signaling plays in this important morphogenetic process.http://bio.biologists.org/content/6/2/232METBlood-brain barrierGPCR signalingEpithelial morphogenesisDrosophila |
| spellingShingle | Tina Schwabe Xiaoling Li Ulrike Gaul Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila Biology Open MET Blood-brain barrier GPCR signaling Epithelial morphogenesis Drosophila |
| title | Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila |
| title_full | Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila |
| title_fullStr | Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila |
| title_full_unstemmed | Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila |
| title_short | Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila |
| title_sort | dynamic analysis of the mesenchymal epithelial transition of blood brain barrier forming glia in drosophila |
| topic | MET Blood-brain barrier GPCR signaling Epithelial morphogenesis Drosophila |
| url | http://bio.biologists.org/content/6/2/232 |
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