Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice
<p>Abstract</p> <p>Background</p> <p>Fuzheng Huayu recipe (FZHY), a compound of Chinese herbal medicine, was reported to improve liver function and fibrosis in patients with hepatitis B virus infection. However, its effect on nutritional fibrosing steatohepatitis is unc...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-03-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://www.lipidworld.com/content/11/1/45 |
| _version_ | 1811293284124852224 |
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| author | Jia Yan-Hong Wang Rong-Qi Mi Hong-Mei Kong Ling-Bo Ren Wei-Guang Li Wen-Cong Zhao Su-Xian Zhang Yu-Guo Wu Wen-Juan Nan Yue-Min Yu Jun |
| author_facet | Jia Yan-Hong Wang Rong-Qi Mi Hong-Mei Kong Ling-Bo Ren Wei-Guang Li Wen-Cong Zhao Su-Xian Zhang Yu-Guo Wu Wen-Juan Nan Yue-Min Yu Jun |
| author_sort | Jia Yan-Hong |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Fuzheng Huayu recipe (FZHY), a compound of Chinese herbal medicine, was reported to improve liver function and fibrosis in patients with hepatitis B virus infection. However, its effect on nutritional fibrosing steatohepatitis is unclear. We aimed to elucidate the role and molecular mechanism of FZHY on this disorder in mice.</p> <p>Methods</p> <p>C57BL/6 J mice were fed with methionine-choline deficient (MCD) diet for 8 weeks to induce fibrosing steatohepatitis. FZHY and/or heme oxygenase-1 (HO-1) chemical inducer (hemin) were administered to mice, respectively. The effect of FZHY was assessed by comparing the severity of hepatic injury, levels of hepatic lipid peroxides, activation of hepatic stellate cells (HSCs) and the expression of oxidative stress, inflammatory and fibrogenic related genes.</p> <p>Results</p> <p>Mice fed with MCD diet for 8 weeks showed severe hepatic injury including hepatic steatosis, necro-inflammation and fibrosis. Administration of FZHY or hemin significantly lowered serum levels of alanine aminotransferase, aspartate aminotransferase, reduced hepatic oxidative stress and ameliorated hepatic inflammation and fibrosis. An additive effect was observed in mice fed MCD supplemented with FZHY or/and hemin. These effects were associated with down-regulation of pro-oxidative stress gene cytochrome P450 2E1, up-regulation of anti-oxidative gene HO-1; suppression of pro-inflammation genes tumor necrosis factor alpha and interleukin-6; and inhibition of pro-fibrotic genes including α-smooth muscle actin, transforming growth factor beta 1, collagen type I (Col-1) and Col-3.</p> <p>Conclusions</p> <p>Our study demonstrated the protective role of FZHY in ameliorating nutritional fibrosing steatohepatitis. The effect was mediated through regulating key genes related to oxidative stress, inflammation and fibrogenesis.</p> |
| first_indexed | 2024-04-13T04:59:07Z |
| format | Article |
| id | doaj.art-a3426d3dafe344c3bd8a77130dff8976 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-04-13T04:59:07Z |
| publishDate | 2012-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-a3426d3dafe344c3bd8a77130dff89762022-12-22T03:01:23ZengBMCLipids in Health and Disease1476-511X2012-03-011114510.1186/1476-511X-11-45Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in miceJia Yan-HongWang Rong-QiMi Hong-MeiKong Ling-BoRen Wei-GuangLi Wen-CongZhao Su-XianZhang Yu-GuoWu Wen-JuanNan Yue-MinYu Jun<p>Abstract</p> <p>Background</p> <p>Fuzheng Huayu recipe (FZHY), a compound of Chinese herbal medicine, was reported to improve liver function and fibrosis in patients with hepatitis B virus infection. However, its effect on nutritional fibrosing steatohepatitis is unclear. We aimed to elucidate the role and molecular mechanism of FZHY on this disorder in mice.</p> <p>Methods</p> <p>C57BL/6 J mice were fed with methionine-choline deficient (MCD) diet for 8 weeks to induce fibrosing steatohepatitis. FZHY and/or heme oxygenase-1 (HO-1) chemical inducer (hemin) were administered to mice, respectively. The effect of FZHY was assessed by comparing the severity of hepatic injury, levels of hepatic lipid peroxides, activation of hepatic stellate cells (HSCs) and the expression of oxidative stress, inflammatory and fibrogenic related genes.</p> <p>Results</p> <p>Mice fed with MCD diet for 8 weeks showed severe hepatic injury including hepatic steatosis, necro-inflammation and fibrosis. Administration of FZHY or hemin significantly lowered serum levels of alanine aminotransferase, aspartate aminotransferase, reduced hepatic oxidative stress and ameliorated hepatic inflammation and fibrosis. An additive effect was observed in mice fed MCD supplemented with FZHY or/and hemin. These effects were associated with down-regulation of pro-oxidative stress gene cytochrome P450 2E1, up-regulation of anti-oxidative gene HO-1; suppression of pro-inflammation genes tumor necrosis factor alpha and interleukin-6; and inhibition of pro-fibrotic genes including α-smooth muscle actin, transforming growth factor beta 1, collagen type I (Col-1) and Col-3.</p> <p>Conclusions</p> <p>Our study demonstrated the protective role of FZHY in ameliorating nutritional fibrosing steatohepatitis. The effect was mediated through regulating key genes related to oxidative stress, inflammation and fibrogenesis.</p>http://www.lipidworld.com/content/11/1/45FuzhengHuayuChinese herbNon-alcoholic steatohepatitisHepatic fibrosisOxidative stress |
| spellingShingle | Jia Yan-Hong Wang Rong-Qi Mi Hong-Mei Kong Ling-Bo Ren Wei-Guang Li Wen-Cong Zhao Su-Xian Zhang Yu-Guo Wu Wen-Juan Nan Yue-Min Yu Jun Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice Lipids in Health and Disease FuzhengHuayu Chinese herb Non-alcoholic steatohepatitis Hepatic fibrosis Oxidative stress |
| title | Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice |
| title_full | Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice |
| title_fullStr | Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice |
| title_full_unstemmed | Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice |
| title_short | Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice |
| title_sort | fuzheng huayu recipe prevents nutritional fibrosing steatohepatitis in mice |
| topic | FuzhengHuayu Chinese herb Non-alcoholic steatohepatitis Hepatic fibrosis Oxidative stress |
| url | http://www.lipidworld.com/content/11/1/45 |
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