DNA barcoding, population genetics, and phylogenetics of the illegally hunted Philippine Duck Anas luzonica (Aves: Anseriformes: Anatidae)
DNA barcoding is extensively used as a species identification and delineation tool. The aim of this study was to generate a barcode profile for mitochondrial cytochrome c oxidase subunit 1 (COI) in the Philippine Duck Anas luzonica, a dabbling duck species endemic to the Philippines that is classif...
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| Format: | Article |
| Language: | English |
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Wildlife Information Liaison Development Society
2017-05-01
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| Series: | Journal of Threatened Taxa |
| Subjects: | |
| Online Access: | https://www.threatenedtaxa.org/index.php/JoTT/article/view/2253 |
| _version_ | 1818836373006712832 |
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| author | Ardea Mingoa Licuanan Mariano RoyMartin Duya Perry Sim Ong Ian Kendrich Carandang Fontanilla |
| author_facet | Ardea Mingoa Licuanan Mariano RoyMartin Duya Perry Sim Ong Ian Kendrich Carandang Fontanilla |
| author_sort | Ardea Mingoa Licuanan |
| collection | DOAJ |
| description | DNA barcoding is extensively used as a species identification and delineation tool. The aim of this study was to generate a barcode profile for mitochondrial cytochrome c oxidase subunit 1 (COI) in the Philippine Duck Anas luzonica, a dabbling duck species endemic to the Philippines that is classified as ‘Vulnerable’ by the International Union for Conservation of Nature (BirdLife International 2016). COI barcodes were successfully obtained using muscle tissue samples from 46 A. luzonica individuals confiscated from illegal hunters in Pantabangan, Nueva Ecija. Analysis of TrN+Γ+I distances among the Anas luzonica COI sequences and those of 25 other Anas species revealed that COI barcodes cannot generally delineate hybridizing species. While Anas luzonica was differentiated from other species it is known to hybridize with and formed a monophyletic group in the neighbor-joining tree generated, sampling from areas of sympatry is needed since individuals were obtained from only one sampling site. The population structure of the Anas luzonica population was also examined using mitochondrial DNA control region and COI sequences. The population had high haplotype diversity and low nucleotide diversity, an indication that a bottleneck event had occurred, which is likely due to extreme hunting pressures and habitat destruction. The population under study exhibited high genetic diversity. Given that the samples for this study came from a single locality, sampling from other localities is required to determine whether other populations are facing the risk of reduced fitness (inbreeding depression). |
| first_indexed | 2024-12-19T03:05:34Z |
| format | Article |
| id | doaj.art-a344eff1f51f45d8b91b3303b732906f |
| institution | Directory Open Access Journal |
| issn | 0974-7893 0974-7907 |
| language | English |
| last_indexed | 2024-12-19T03:05:34Z |
| publishDate | 2017-05-01 |
| publisher | Wildlife Information Liaison Development Society |
| record_format | Article |
| series | Journal of Threatened Taxa |
| spelling | doaj.art-a344eff1f51f45d8b91b3303b732906f2022-12-21T20:38:08ZengWildlife Information Liaison Development SocietyJournal of Threatened Taxa0974-78930974-79072017-05-0195101411015010.11609/jott.2253.9.5.10141-101502253DNA barcoding, population genetics, and phylogenetics of the illegally hunted Philippine Duck Anas luzonica (Aves: Anseriformes: Anatidae)Ardea Mingoa Licuanan0Mariano RoyMartin Duya1Perry Sim Ong2Ian Kendrich Carandang Fontanilla3Institute of Biology, University of the Philippines, Diliman, Quezon City 1101, PhilippinesInstitute of Biology, University of the Philippines, Diliman, Quezon City 1101, PhilippinesInstitute of Biology, University of the Philippines, Diliman, Quezon City 1101, PhilippinesInstitute of Biology, University of the Philippines, Diliman, Quezon City 1101, Philippines & Philippine Genome Center, University of the Philippines, Diliman, Quezon City 1101, PhilippinesDNA barcoding is extensively used as a species identification and delineation tool. The aim of this study was to generate a barcode profile for mitochondrial cytochrome c oxidase subunit 1 (COI) in the Philippine Duck Anas luzonica, a dabbling duck species endemic to the Philippines that is classified as ‘Vulnerable’ by the International Union for Conservation of Nature (BirdLife International 2016). COI barcodes were successfully obtained using muscle tissue samples from 46 A. luzonica individuals confiscated from illegal hunters in Pantabangan, Nueva Ecija. Analysis of TrN+Γ+I distances among the Anas luzonica COI sequences and those of 25 other Anas species revealed that COI barcodes cannot generally delineate hybridizing species. While Anas luzonica was differentiated from other species it is known to hybridize with and formed a monophyletic group in the neighbor-joining tree generated, sampling from areas of sympatry is needed since individuals were obtained from only one sampling site. The population structure of the Anas luzonica population was also examined using mitochondrial DNA control region and COI sequences. The population had high haplotype diversity and low nucleotide diversity, an indication that a bottleneck event had occurred, which is likely due to extreme hunting pressures and habitat destruction. The population under study exhibited high genetic diversity. Given that the samples for this study came from a single locality, sampling from other localities is required to determine whether other populations are facing the risk of reduced fitness (inbreeding depression).https://www.threatenedtaxa.org/index.php/JoTT/article/view/2253anas luzonicadna barcodingpopulation bottleneck. |
| spellingShingle | Ardea Mingoa Licuanan Mariano RoyMartin Duya Perry Sim Ong Ian Kendrich Carandang Fontanilla DNA barcoding, population genetics, and phylogenetics of the illegally hunted Philippine Duck Anas luzonica (Aves: Anseriformes: Anatidae) Journal of Threatened Taxa anas luzonica dna barcoding population bottleneck. |
| title | DNA barcoding, population genetics, and phylogenetics of the illegally hunted Philippine Duck Anas luzonica (Aves: Anseriformes: Anatidae) |
| title_full | DNA barcoding, population genetics, and phylogenetics of the illegally hunted Philippine Duck Anas luzonica (Aves: Anseriformes: Anatidae) |
| title_fullStr | DNA barcoding, population genetics, and phylogenetics of the illegally hunted Philippine Duck Anas luzonica (Aves: Anseriformes: Anatidae) |
| title_full_unstemmed | DNA barcoding, population genetics, and phylogenetics of the illegally hunted Philippine Duck Anas luzonica (Aves: Anseriformes: Anatidae) |
| title_short | DNA barcoding, population genetics, and phylogenetics of the illegally hunted Philippine Duck Anas luzonica (Aves: Anseriformes: Anatidae) |
| title_sort | dna barcoding population genetics and phylogenetics of the illegally hunted philippine duck anas luzonica aves anseriformes anatidae |
| topic | anas luzonica dna barcoding population bottleneck. |
| url | https://www.threatenedtaxa.org/index.php/JoTT/article/view/2253 |
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