Decellularized squid mantle scaffolds as tissue‐engineered corneal stroma for promoting corneal regeneration
Abstract Corneal blindness is a worldwide major cause of vision loss, and corneal transplantation remains to be the most effective way to restore the vision. However, often there is a shortage of the donor corneas for transplantation. Therefore, it is urgent to develop a novel tissue‐engineered corn...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-07-01
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| Series: | Bioengineering & Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/btm2.10531 |
| _version_ | 1797777292333678592 |
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| author | Honghua Kang Yi Han Mengyi Jin Lan Zheng Zhen Liu Yuhua Xue Zuguo Liu Cheng Li |
| author_facet | Honghua Kang Yi Han Mengyi Jin Lan Zheng Zhen Liu Yuhua Xue Zuguo Liu Cheng Li |
| author_sort | Honghua Kang |
| collection | DOAJ |
| description | Abstract Corneal blindness is a worldwide major cause of vision loss, and corneal transplantation remains to be the most effective way to restore the vision. However, often there is a shortage of the donor corneas for transplantation. Therefore, it is urgent to develop a novel tissue‐engineered corneal substitute. The present study envisaged the development of a novel and efficient method to prepare the corneal stromal equivalent from the marine biomaterials‐squid. A chemical method was employed to decellularize the squid mantle scaffold to create a cell‐free tissue substitute using 0.5% sodium dodecyl sulfate (SDS) solution. Subsequently, a novel clearing method, namely clear, unobstructed brain imaging cocktails (CUBIC) method was used to transparent it. Decellularized squid mantle scaffold (DSMS) has high decellularization efficiency, is rich in essential amino acids, and maintains the regular fiber alignment. In vitro experiments showed that the soaking solution of DSMS was non‐toxic to human corneal epithelium cells. DSMS exhibited a good biocompatibility in the rat muscle by undergoing a complete degradation, and promoted the growth of the muscle. In addition, the DSMS showed a good compatibility with the corneal stroma in the rabbit inter‐corneal implantation model, and promoted the regeneration of the corneal stroma without any evident rejection. Our results indicate that the squid mantle can be a potential new type of tissue‐engineered corneal stroma material with a promising clinical application. |
| first_indexed | 2024-03-12T23:02:00Z |
| format | Article |
| id | doaj.art-a345128f011445a585f08bc1d8a63bd4 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-03-12T23:02:00Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-a345128f011445a585f08bc1d8a63bd42023-07-19T09:59:03ZengWileyBioengineering & Translational Medicine2380-67612023-07-0184n/an/a10.1002/btm2.10531Decellularized squid mantle scaffolds as tissue‐engineered corneal stroma for promoting corneal regenerationHonghua Kang0Yi Han1Mengyi Jin2Lan Zheng3Zhen Liu4Yuhua Xue5Zuguo Liu6Cheng Li7Eye Institute & Affiliated Xiamen Eye Center, School of Medicine Xiamen University Xiamen ChinaEye Institute & Affiliated Xiamen Eye Center, School of Medicine Xiamen University Xiamen ChinaEye Institute & Affiliated Xiamen Eye Center, School of Medicine Xiamen University Xiamen ChinaEye Institute & Affiliated Xiamen Eye Center, School of Medicine Xiamen University Xiamen ChinaEye Institute & Affiliated Xiamen Eye Center, School of Medicine Xiamen University Xiamen ChinaSchool of Pharmaceutical Sciences Xiamen University Xiamen ChinaEye Institute & Affiliated Xiamen Eye Center, School of Medicine Xiamen University Xiamen ChinaEye Institute & Affiliated Xiamen Eye Center, School of Medicine Xiamen University Xiamen ChinaAbstract Corneal blindness is a worldwide major cause of vision loss, and corneal transplantation remains to be the most effective way to restore the vision. However, often there is a shortage of the donor corneas for transplantation. Therefore, it is urgent to develop a novel tissue‐engineered corneal substitute. The present study envisaged the development of a novel and efficient method to prepare the corneal stromal equivalent from the marine biomaterials‐squid. A chemical method was employed to decellularize the squid mantle scaffold to create a cell‐free tissue substitute using 0.5% sodium dodecyl sulfate (SDS) solution. Subsequently, a novel clearing method, namely clear, unobstructed brain imaging cocktails (CUBIC) method was used to transparent it. Decellularized squid mantle scaffold (DSMS) has high decellularization efficiency, is rich in essential amino acids, and maintains the regular fiber alignment. In vitro experiments showed that the soaking solution of DSMS was non‐toxic to human corneal epithelium cells. DSMS exhibited a good biocompatibility in the rat muscle by undergoing a complete degradation, and promoted the growth of the muscle. In addition, the DSMS showed a good compatibility with the corneal stroma in the rabbit inter‐corneal implantation model, and promoted the regeneration of the corneal stroma without any evident rejection. Our results indicate that the squid mantle can be a potential new type of tissue‐engineered corneal stroma material with a promising clinical application.https://doi.org/10.1002/btm2.10531corneal stromal equivalentscorneal tissue engineeringdecellularized squid mantlemarine biomaterialstissue clearing technology |
| spellingShingle | Honghua Kang Yi Han Mengyi Jin Lan Zheng Zhen Liu Yuhua Xue Zuguo Liu Cheng Li Decellularized squid mantle scaffolds as tissue‐engineered corneal stroma for promoting corneal regeneration Bioengineering & Translational Medicine corneal stromal equivalents corneal tissue engineering decellularized squid mantle marine biomaterials tissue clearing technology |
| title | Decellularized squid mantle scaffolds as tissue‐engineered corneal stroma for promoting corneal regeneration |
| title_full | Decellularized squid mantle scaffolds as tissue‐engineered corneal stroma for promoting corneal regeneration |
| title_fullStr | Decellularized squid mantle scaffolds as tissue‐engineered corneal stroma for promoting corneal regeneration |
| title_full_unstemmed | Decellularized squid mantle scaffolds as tissue‐engineered corneal stroma for promoting corneal regeneration |
| title_short | Decellularized squid mantle scaffolds as tissue‐engineered corneal stroma for promoting corneal regeneration |
| title_sort | decellularized squid mantle scaffolds as tissue engineered corneal stroma for promoting corneal regeneration |
| topic | corneal stromal equivalents corneal tissue engineering decellularized squid mantle marine biomaterials tissue clearing technology |
| url | https://doi.org/10.1002/btm2.10531 |
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