Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression.
In the present work it was investigated if a spontaneous alteration of the native melanotic transplantable melanoma form into amelanotic form, connected with the tumor progression, is accompanied by changes of CD44 surface glycoprotein expression. We also tried to find out if there exists any correl...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Via Medica
2004-03-01
|
| Series: | Folia Histochemica et Cytobiologica |
| Subjects: | |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4664 |
| _version_ | 1818031010652094464 |
|---|---|
| author | Sławomir Wójcik Małgorzata Zarzeczna Mirosława Cichorek Krystyna Kozłowska |
| author_facet | Sławomir Wójcik Małgorzata Zarzeczna Mirosława Cichorek Krystyna Kozłowska |
| author_sort | Sławomir Wójcik |
| collection | DOAJ |
| description | In the present work it was investigated if a spontaneous alteration of the native melanotic transplantable melanoma form into amelanotic form, connected with the tumor progression, is accompanied by changes of CD44 surface glycoprotein expression. We also tried to find out if there exists any correlation between changes in CD44 expression and IL-6, TNF-alpha, and IL-10 secretion. Cells of two hamster transplantable melanoma lines: melanotic and amelanotic were used. The levels of TNF-alpha, IL-6, IL-10 in supernatants were determined by the ELISA test. For the detection of CD44 expression by flow cytometry, isolated melanoma cells were stained with the rat anti-mouse CD44 monoclonal antibody. The stained cells were also examined using a fluorescence microscope and a confocal microscopy system. The obtained results indicate that a spontaneous alteration of the native melanotic form into amelanotic form and the associated tumor progression was accompanied by a decrease in CD44 glycoprotein expression on the cell surface and a decrease in IL-6, TNF-alpha and especially IL-10 secretion by amelanotic melanoma cells. Our observations suggest a relationship between CD44 expression and locally secreted cytokines in the course of transplantable melanoma progression. |
| first_indexed | 2024-12-10T05:44:41Z |
| format | Article |
| id | doaj.art-a345bdd9d950418a9b094af22f522918 |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-12-10T05:44:41Z |
| publishDate | 2004-03-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-a345bdd9d950418a9b094af22f5229182022-12-22T02:00:12ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312004-03-01421293410.5603/4664Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression.Sławomir WójcikMałgorzata ZarzecznaMirosława CichorekKrystyna KozłowskaIn the present work it was investigated if a spontaneous alteration of the native melanotic transplantable melanoma form into amelanotic form, connected with the tumor progression, is accompanied by changes of CD44 surface glycoprotein expression. We also tried to find out if there exists any correlation between changes in CD44 expression and IL-6, TNF-alpha, and IL-10 secretion. Cells of two hamster transplantable melanoma lines: melanotic and amelanotic were used. The levels of TNF-alpha, IL-6, IL-10 in supernatants were determined by the ELISA test. For the detection of CD44 expression by flow cytometry, isolated melanoma cells were stained with the rat anti-mouse CD44 monoclonal antibody. The stained cells were also examined using a fluorescence microscope and a confocal microscopy system. The obtained results indicate that a spontaneous alteration of the native melanotic form into amelanotic form and the associated tumor progression was accompanied by a decrease in CD44 glycoprotein expression on the cell surface and a decrease in IL-6, TNF-alpha and especially IL-10 secretion by amelanotic melanoma cells. Our observations suggest a relationship between CD44 expression and locally secreted cytokines in the course of transplantable melanoma progression.http://czasopisma.viamedica.pl/fhc/article/view/4664CD44Transplantable melanomasCytokinesIL-6 - IL-10TNF-α |
| spellingShingle | Sławomir Wójcik Małgorzata Zarzeczna Mirosława Cichorek Krystyna Kozłowska Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression. Folia Histochemica et Cytobiologica CD44 Transplantable melanomas Cytokines IL-6 - IL-10 TNF-α |
| title | Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression. |
| title_full | Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression. |
| title_fullStr | Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression. |
| title_full_unstemmed | Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression. |
| title_short | Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression. |
| title_sort | expression of cd44 on two lines of transplantable melanoma cells relationship with cytokine secretion and tumor progression |
| topic | CD44 Transplantable melanomas Cytokines IL-6 - IL-10 TNF-α |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4664 |
| work_keys_str_mv | AT sławomirwojcik expressionofcd44ontwolinesoftransplantablemelanomacellsrelationshipwithcytokinesecretionandtumorprogression AT małgorzatazarzeczna expressionofcd44ontwolinesoftransplantablemelanomacellsrelationshipwithcytokinesecretionandtumorprogression AT mirosławacichorek expressionofcd44ontwolinesoftransplantablemelanomacellsrelationshipwithcytokinesecretionandtumorprogression AT krystynakozłowska expressionofcd44ontwolinesoftransplantablemelanomacellsrelationshipwithcytokinesecretionandtumorprogression |