Induction of nano pore in Agrobacterial hemoglobin
Introduction: A variety of oxygen-transport and -binding proteins exist in organisms including bacteria, protozoans, and fungi all have hemoglobin-like proteins. In addition to dealing with transport and sensing of oxygen, they may also deal with NO2, CO2, sulfide compounds, and even O2 scavenging i...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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University of Isfahan
2014-01-01
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| Series: | Biological Journal of Microorganism |
| Subjects: | |
| Online Access: | http://uijs.ui.ac.ir/bjm/browse.php?a_id=66&slc_lang=en&sid=1&ftxt=1 |
| _version_ | 1818664969899606016 |
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| author | Mojtaba Tousheh Giti Emtiazi Peyman Derikvand |
| author_facet | Mojtaba Tousheh Giti Emtiazi Peyman Derikvand |
| author_sort | Mojtaba Tousheh |
| collection | DOAJ |
| description | Introduction: A variety of oxygen-transport and -binding proteins exist in organisms including bacteria, protozoans, and fungi all have hemoglobin-like proteins. In addition to dealing with transport and sensing of oxygen, they may also deal with NO2, CO2, sulfide compounds, and even O2 scavenging in environments. Also they detoxified chlorinated materials like P450 enzymes and peroxidases and use as a detector of nitrate and hydrogen peroxide. Pore-forming bacterial globins are interested for filtration.
Materials and methods: Although there are data for bacterial toxin as a filter, here we used Agrobacterial hem to induce nano pore in the heme structure using point mutation.
Results: Investigations showed that three amino acids leucine 76, alanine 83 and histidine 80 are important for pore formation in Agrobacterium hemoglobin. A point mutation on leucine 76 to glycine, histidine 80 to asparagine and alanine 83 to lysine step by step led to create the nano pore 0.7- 0.8 nm in the globin.
Discussion and conclusion: These mutations in bacterial hemoglobin increase the stability when mutation is with it’s at pH7. This mutation decreases the aliphatic index however increase the stability index. |
| first_indexed | 2024-12-17T05:41:11Z |
| format | Article |
| id | doaj.art-a349ce842327401fb01d02f72278f7bd |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-12-17T05:41:11Z |
| publishDate | 2014-01-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-a349ce842327401fb01d02f72278f7bd2022-12-21T22:01:27ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812014-01-01282328Induction of nano pore in Agrobacterial hemoglobinMojtaba Tousheh 0Giti Emtiazi 1Peyman Derikvand2M.Sc. of Cellular and Molecular Biology, University of Isfahan, Iran, mojtaba.tousheh@gmail.comFull professor of Microbiology, university of Isfahan, Iran, emtiazi@yahoo.com M.Sc. of Microbiology, University of Isfahan, Iran, p.derikvand@gmail.comIntroduction: A variety of oxygen-transport and -binding proteins exist in organisms including bacteria, protozoans, and fungi all have hemoglobin-like proteins. In addition to dealing with transport and sensing of oxygen, they may also deal with NO2, CO2, sulfide compounds, and even O2 scavenging in environments. Also they detoxified chlorinated materials like P450 enzymes and peroxidases and use as a detector of nitrate and hydrogen peroxide. Pore-forming bacterial globins are interested for filtration. Materials and methods: Although there are data for bacterial toxin as a filter, here we used Agrobacterial hem to induce nano pore in the heme structure using point mutation. Results: Investigations showed that three amino acids leucine 76, alanine 83 and histidine 80 are important for pore formation in Agrobacterium hemoglobin. A point mutation on leucine 76 to glycine, histidine 80 to asparagine and alanine 83 to lysine step by step led to create the nano pore 0.7- 0.8 nm in the globin. Discussion and conclusion: These mutations in bacterial hemoglobin increase the stability when mutation is with it’s at pH7. This mutation decreases the aliphatic index however increase the stability index.http://uijs.ui.ac.ir/bjm/browse.php?a_id=66&slc_lang=en&sid=1&ftxt=1HemoglobinAgrobacteriumMolegro Virtual Docker |
| spellingShingle | Mojtaba Tousheh Giti Emtiazi Peyman Derikvand Induction of nano pore in Agrobacterial hemoglobin Biological Journal of Microorganism Hemoglobin Agrobacterium Molegro Virtual Docker |
| title | Induction of nano pore in Agrobacterial hemoglobin |
| title_full | Induction of nano pore in Agrobacterial hemoglobin |
| title_fullStr | Induction of nano pore in Agrobacterial hemoglobin |
| title_full_unstemmed | Induction of nano pore in Agrobacterial hemoglobin |
| title_short | Induction of nano pore in Agrobacterial hemoglobin |
| title_sort | induction of nano pore in agrobacterial hemoglobin |
| topic | Hemoglobin Agrobacterium Molegro Virtual Docker |
| url | http://uijs.ui.ac.ir/bjm/browse.php?a_id=66&slc_lang=en&sid=1&ftxt=1 |
| work_keys_str_mv | AT mojtabatousheh inductionofnanoporeinagrobacterialhemoglobin AT gitiemtiazi inductionofnanoporeinagrobacterialhemoglobin AT peymanderikvand inductionofnanoporeinagrobacterialhemoglobin |