Non-Invasive Physical Plasma Generated by a Medical Argon Plasma Device Induces the Expression of Regenerative Factors in Human Gingival Keratinocytes, Fibroblasts, and Tissue Biopsies
After oral surgery, intraoral wound healing and tissue regeneration is an important factor for the success of the entire therapy. In recent years, non-invasive medical plasma (NIPP) has been shown to accelerate wound healing, which would be particularly beneficial for patients with wound healing dis...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/4/889 |
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| author | Benedikt Eggers Matthias Bernhard Stope Jana Marciniak Werner Götz Alexander Mustea James Deschner Marjan Nokhbehsaim Franz-Josef Kramer |
| author_facet | Benedikt Eggers Matthias Bernhard Stope Jana Marciniak Werner Götz Alexander Mustea James Deschner Marjan Nokhbehsaim Franz-Josef Kramer |
| author_sort | Benedikt Eggers |
| collection | DOAJ |
| description | After oral surgery, intraoral wound healing and tissue regeneration is an important factor for the success of the entire therapy. In recent years, non-invasive medical plasma (NIPP) has been shown to accelerate wound healing, which would be particularly beneficial for patients with wound healing disorders. Since the application of NIPP in dentistry has not been sufficiently understood, the aim of the present study was to investigate the effect of a medical argon plasma device on gingival cells. Human gingival fibroblasts, keratinocytes, and tissue biopsies were treated with NIPP for different durations. Crucial markers associated with wound healing were examined at the mRNA and protein levels by real-time PCR, ELISA and immunohistochemistry. NIPP treatment led to an increase in Ki67 and MMP1 at mRNA and protein levels. NIPP application lasting longer than 60 s resulted in an increase in apoptotic genes at mRNA level and superficial damage to the epithelium in the tissue biopsies. Overall, our experimental setup demonstrated that NIPP application times of 30 s were most suitable for the treatment of gingival cells and tissue biopsies. Our study provides evidence for potential use of NIPP in dentistry, which would be a promising treatment option for oral surgery. |
| first_indexed | 2024-03-09T11:07:21Z |
| format | Article |
| id | doaj.art-a34a722d5abd46c6bb84b3a1e0efebba |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T11:07:21Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-a34a722d5abd46c6bb84b3a1e0efebba2023-12-01T00:54:55ZengMDPI AGBiomedicines2227-90592022-04-0110488910.3390/biomedicines10040889Non-Invasive Physical Plasma Generated by a Medical Argon Plasma Device Induces the Expression of Regenerative Factors in Human Gingival Keratinocytes, Fibroblasts, and Tissue BiopsiesBenedikt Eggers0Matthias Bernhard Stope1Jana Marciniak2Werner Götz3Alexander Mustea4James Deschner5Marjan Nokhbehsaim6Franz-Josef Kramer7Department of Oral, Maxillofacial and Plastic Surgery, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Gynecology and Gynecological Oncology, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Orthodontics, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Orthodontics, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Gynecology and Gynecological Oncology, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, GermanySection of Experimental Dento-Maxillo-Facial Medicine, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Oral, Maxillofacial and Plastic Surgery, University Hospital Bonn, 53111 Bonn, GermanyAfter oral surgery, intraoral wound healing and tissue regeneration is an important factor for the success of the entire therapy. In recent years, non-invasive medical plasma (NIPP) has been shown to accelerate wound healing, which would be particularly beneficial for patients with wound healing disorders. Since the application of NIPP in dentistry has not been sufficiently understood, the aim of the present study was to investigate the effect of a medical argon plasma device on gingival cells. Human gingival fibroblasts, keratinocytes, and tissue biopsies were treated with NIPP for different durations. Crucial markers associated with wound healing were examined at the mRNA and protein levels by real-time PCR, ELISA and immunohistochemistry. NIPP treatment led to an increase in Ki67 and MMP1 at mRNA and protein levels. NIPP application lasting longer than 60 s resulted in an increase in apoptotic genes at mRNA level and superficial damage to the epithelium in the tissue biopsies. Overall, our experimental setup demonstrated that NIPP application times of 30 s were most suitable for the treatment of gingival cells and tissue biopsies. Our study provides evidence for potential use of NIPP in dentistry, which would be a promising treatment option for oral surgery.https://www.mdpi.com/2227-9059/10/4/889non-invasive physical plasmacold atmospheric plasmacold atmospheric pressure plasmagingival keratinocytesgingival fibroblaststissue biopsies |
| spellingShingle | Benedikt Eggers Matthias Bernhard Stope Jana Marciniak Werner Götz Alexander Mustea James Deschner Marjan Nokhbehsaim Franz-Josef Kramer Non-Invasive Physical Plasma Generated by a Medical Argon Plasma Device Induces the Expression of Regenerative Factors in Human Gingival Keratinocytes, Fibroblasts, and Tissue Biopsies Biomedicines non-invasive physical plasma cold atmospheric plasma cold atmospheric pressure plasma gingival keratinocytes gingival fibroblasts tissue biopsies |
| title | Non-Invasive Physical Plasma Generated by a Medical Argon Plasma Device Induces the Expression of Regenerative Factors in Human Gingival Keratinocytes, Fibroblasts, and Tissue Biopsies |
| title_full | Non-Invasive Physical Plasma Generated by a Medical Argon Plasma Device Induces the Expression of Regenerative Factors in Human Gingival Keratinocytes, Fibroblasts, and Tissue Biopsies |
| title_fullStr | Non-Invasive Physical Plasma Generated by a Medical Argon Plasma Device Induces the Expression of Regenerative Factors in Human Gingival Keratinocytes, Fibroblasts, and Tissue Biopsies |
| title_full_unstemmed | Non-Invasive Physical Plasma Generated by a Medical Argon Plasma Device Induces the Expression of Regenerative Factors in Human Gingival Keratinocytes, Fibroblasts, and Tissue Biopsies |
| title_short | Non-Invasive Physical Plasma Generated by a Medical Argon Plasma Device Induces the Expression of Regenerative Factors in Human Gingival Keratinocytes, Fibroblasts, and Tissue Biopsies |
| title_sort | non invasive physical plasma generated by a medical argon plasma device induces the expression of regenerative factors in human gingival keratinocytes fibroblasts and tissue biopsies |
| topic | non-invasive physical plasma cold atmospheric plasma cold atmospheric pressure plasma gingival keratinocytes gingival fibroblasts tissue biopsies |
| url | https://www.mdpi.com/2227-9059/10/4/889 |
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