Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability
Some aquaporins (AQPs) allow the diffusion of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), the most abundant ROS, through the cell membranes. Therefore, the possibility of regulating the AQP-mediated permeability to H<sub>2</sub>O<sub>2</sub>, and t...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Giorgia Pellavio Patrizia Sommi Umberto Anselmi-Tamburini Maria Paola DeMichelis Stefania Coniglio Umberto Laforenza |
| author_facet | Giorgia Pellavio Patrizia Sommi Umberto Anselmi-Tamburini Maria Paola DeMichelis Stefania Coniglio Umberto Laforenza |
| author_sort | Giorgia Pellavio |
| collection | DOAJ |
| description | Some aquaporins (AQPs) allow the diffusion of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), the most abundant ROS, through the cell membranes. Therefore, the possibility of regulating the AQP-mediated permeability to H<sub>2</sub>O<sub>2</sub>, and thus ROS scavenging, appears particularly important for controlling the redox state of cells in physiological and pathophysiological conditions. Several compounds have been screened and characterized for this purpose. This study aimed to analyze the effect of cerium oxide nanoparticles (CNPs) presenting antioxidant activity on AQP functioning. HeLa cells express AQP3, 6, 8, and 11, able to facilitate H<sub>2</sub>O<sub>2</sub>. AQP3, 6, and 8 are expressed in the plasma membrane and intracellularly, while AQP11 resides only in intracellular structures. CNPs but not cerium ions treatment significantly increased the water and H<sub>2</sub>O<sub>2</sub> permeability by interacting with AQP3, 6, and especially with AQP8. CNPs increased considerably the AQP-mediated water diffusion in cells with oxidative stress. Functional experiments with silenced HeLa cells revealed that CNPs increased the H<sub>2</sub>O<sub>2</sub> diffusion mainly by modulating the AQP8 permeability but also the AQP3 and AQP6, even if to a lesser extent. Current findings suggest that CNPs represent a promising pharmaceutical agent that might potentially be used in numerous pathologies involving oxidative stress as tumors and neurodegenerative diseases. |
| first_indexed | 2024-03-09T23:43:03Z |
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| language | English |
| last_indexed | 2024-03-09T23:43:03Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a34ae24d57364ff58269a1c76d160c302023-11-23T16:48:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181083710.3390/ijms231810837Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide PermeabilityGiorgia Pellavio0Patrizia Sommi1Umberto Anselmi-Tamburini2Maria Paola DeMichelis3Stefania Coniglio4Umberto Laforenza5Department of Molecular Medicine, Human Physiology Unit, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Human Physiology Unit, University of Pavia, 27100 Pavia, ItalyDepartment of Chemistry, University of Pavia, 27100 Pavia, ItalyDepartment of Chemistry, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Human Physiology Unit, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Human Physiology Unit, University of Pavia, 27100 Pavia, ItalySome aquaporins (AQPs) allow the diffusion of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), the most abundant ROS, through the cell membranes. Therefore, the possibility of regulating the AQP-mediated permeability to H<sub>2</sub>O<sub>2</sub>, and thus ROS scavenging, appears particularly important for controlling the redox state of cells in physiological and pathophysiological conditions. Several compounds have been screened and characterized for this purpose. This study aimed to analyze the effect of cerium oxide nanoparticles (CNPs) presenting antioxidant activity on AQP functioning. HeLa cells express AQP3, 6, 8, and 11, able to facilitate H<sub>2</sub>O<sub>2</sub>. AQP3, 6, and 8 are expressed in the plasma membrane and intracellularly, while AQP11 resides only in intracellular structures. CNPs but not cerium ions treatment significantly increased the water and H<sub>2</sub>O<sub>2</sub> permeability by interacting with AQP3, 6, and especially with AQP8. CNPs increased considerably the AQP-mediated water diffusion in cells with oxidative stress. Functional experiments with silenced HeLa cells revealed that CNPs increased the H<sub>2</sub>O<sub>2</sub> diffusion mainly by modulating the AQP8 permeability but also the AQP3 and AQP6, even if to a lesser extent. Current findings suggest that CNPs represent a promising pharmaceutical agent that might potentially be used in numerous pathologies involving oxidative stress as tumors and neurodegenerative diseases.https://www.mdpi.com/1422-0067/23/18/10837CeO<sub>2</sub>HeLananoparticleswater channelsperoxiporinHyPer7 probe |
| spellingShingle | Giorgia Pellavio Patrizia Sommi Umberto Anselmi-Tamburini Maria Paola DeMichelis Stefania Coniglio Umberto Laforenza Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability International Journal of Molecular Sciences CeO<sub>2</sub> HeLa nanoparticles water channels peroxiporin HyPer7 probe |
| title | Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability |
| title_full | Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability |
| title_fullStr | Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability |
| title_full_unstemmed | Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability |
| title_short | Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability |
| title_sort | cerium oxide nanoparticles regulate oxidative stress in hela cells by increasing the aquaporin mediated hydrogen peroxide permeability |
| topic | CeO<sub>2</sub> HeLa nanoparticles water channels peroxiporin HyPer7 probe |
| url | https://www.mdpi.com/1422-0067/23/18/10837 |
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