Multiple-locus variable-number tandem-repeat analysis (MLVA) of macrolide-susceptible and -resistant Mycoplasma pneumoniae in children in Taiwan
Background/purpose: To date, molecular typing studies on Mycoplasma pneumoniae are limited. We evaluated the molecular types of Mycoplasma pneumoniae in pediatric patients in Taiwan in 2016. Methods: We used real-time quantitative PCR on respiratory specimens to identify M. pneumoniae in children wi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-10-01
|
| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664619311519 |
| _version_ | 1828403450132561920 |
|---|---|
| author | Chun-Yi Lu Ting-Yu Yen Luan-Ying Chang Yi-Jen Liau Hong-Hsing Liu Li-Min Huang |
| author_facet | Chun-Yi Lu Ting-Yu Yen Luan-Ying Chang Yi-Jen Liau Hong-Hsing Liu Li-Min Huang |
| author_sort | Chun-Yi Lu |
| collection | DOAJ |
| description | Background/purpose: To date, molecular typing studies on Mycoplasma pneumoniae are limited. We evaluated the molecular types of Mycoplasma pneumoniae in pediatric patients in Taiwan in 2016. Methods: We used real-time quantitative PCR on respiratory specimens to identify M. pneumoniae in children with community-acquired pneumonia. The domain V of their 23S rRNA were sequenced for detection of macrolide-resistant point mutations. Molecular typing with multiple locus variable-number tandem repeat analysis (MLVA) was done for both macrolide-susceptible and -resistance M. pneumoniae samples. Results: M. pneumoniae was detected in 22% (180/826) respiratory samples during the study period. Among all M. pneumoniae-positive samples, 24% (43/180) had harbored macrolide-resistant genotypes, and 86% (37/43) of them were A2063G mutation. Forty-two macrolide-resistant strains and 20 randomly selected macrolide-susceptible strains underwent MLVA profiling. MLVA 4-5-7-2 was the most frequent type (32/62, 52%), followed by 4-5-7-3 (17/62, 27%) and 1-5-6-2 (9/62, 15%). There was a strong association between MLVA 4-5-7-2 and macrolide resistance (p < 0.001). In contrast, M 4-5-7-3 and 1-5-6-2 were related to macrolide susceptibility (p < 0.001, and p = 0.025, respectively). Conclusion: Macrolide resistance was relatively low (24%) in this age group in 2016 in Taiwan, and A2063G was the dominant point mutation. MLVA 4-5-7-2 was associated with macrolide resistance. |
| first_indexed | 2024-12-10T10:18:31Z |
| format | Article |
| id | doaj.art-a34afef5079f466a8a245e65e34b4e88 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-10T10:18:31Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-a34afef5079f466a8a245e65e34b4e882022-12-22T01:52:56ZengElsevierJournal of the Formosan Medical Association0929-66462020-10-011191015391545Multiple-locus variable-number tandem-repeat analysis (MLVA) of macrolide-susceptible and -resistant Mycoplasma pneumoniae in children in TaiwanChun-Yi Lu0Ting-Yu Yen1Luan-Ying Chang2Yi-Jen Liau3Hong-Hsing Liu4Li-Min Huang5Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pediatrics, En Chu Kong Hospital, Sanxia District, New Taipei City, Taiwan; Institute of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan Town, Miaoli County, TaiwanDepartment of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan; Corresponding author. No. 8, Chung-Shan South Road, Taipei, 10016, Taiwan. Fax. +886 2 23147450.Background/purpose: To date, molecular typing studies on Mycoplasma pneumoniae are limited. We evaluated the molecular types of Mycoplasma pneumoniae in pediatric patients in Taiwan in 2016. Methods: We used real-time quantitative PCR on respiratory specimens to identify M. pneumoniae in children with community-acquired pneumonia. The domain V of their 23S rRNA were sequenced for detection of macrolide-resistant point mutations. Molecular typing with multiple locus variable-number tandem repeat analysis (MLVA) was done for both macrolide-susceptible and -resistance M. pneumoniae samples. Results: M. pneumoniae was detected in 22% (180/826) respiratory samples during the study period. Among all M. pneumoniae-positive samples, 24% (43/180) had harbored macrolide-resistant genotypes, and 86% (37/43) of them were A2063G mutation. Forty-two macrolide-resistant strains and 20 randomly selected macrolide-susceptible strains underwent MLVA profiling. MLVA 4-5-7-2 was the most frequent type (32/62, 52%), followed by 4-5-7-3 (17/62, 27%) and 1-5-6-2 (9/62, 15%). There was a strong association between MLVA 4-5-7-2 and macrolide resistance (p < 0.001). In contrast, M 4-5-7-3 and 1-5-6-2 were related to macrolide susceptibility (p < 0.001, and p = 0.025, respectively). Conclusion: Macrolide resistance was relatively low (24%) in this age group in 2016 in Taiwan, and A2063G was the dominant point mutation. MLVA 4-5-7-2 was associated with macrolide resistance.http://www.sciencedirect.com/science/article/pii/S0929664619311519Mycoplasma pneumoniaeMLVAMolecular typingMacrolide-resistantTaiwan |
| spellingShingle | Chun-Yi Lu Ting-Yu Yen Luan-Ying Chang Yi-Jen Liau Hong-Hsing Liu Li-Min Huang Multiple-locus variable-number tandem-repeat analysis (MLVA) of macrolide-susceptible and -resistant Mycoplasma pneumoniae in children in Taiwan Journal of the Formosan Medical Association Mycoplasma pneumoniae MLVA Molecular typing Macrolide-resistant Taiwan |
| title | Multiple-locus variable-number tandem-repeat analysis (MLVA) of macrolide-susceptible and -resistant Mycoplasma pneumoniae in children in Taiwan |
| title_full | Multiple-locus variable-number tandem-repeat analysis (MLVA) of macrolide-susceptible and -resistant Mycoplasma pneumoniae in children in Taiwan |
| title_fullStr | Multiple-locus variable-number tandem-repeat analysis (MLVA) of macrolide-susceptible and -resistant Mycoplasma pneumoniae in children in Taiwan |
| title_full_unstemmed | Multiple-locus variable-number tandem-repeat analysis (MLVA) of macrolide-susceptible and -resistant Mycoplasma pneumoniae in children in Taiwan |
| title_short | Multiple-locus variable-number tandem-repeat analysis (MLVA) of macrolide-susceptible and -resistant Mycoplasma pneumoniae in children in Taiwan |
| title_sort | multiple locus variable number tandem repeat analysis mlva of macrolide susceptible and resistant mycoplasma pneumoniae in children in taiwan |
| topic | Mycoplasma pneumoniae MLVA Molecular typing Macrolide-resistant Taiwan |
| url | http://www.sciencedirect.com/science/article/pii/S0929664619311519 |
| work_keys_str_mv | AT chunyilu multiplelocusvariablenumbertandemrepeatanalysismlvaofmacrolidesusceptibleandresistantmycoplasmapneumoniaeinchildrenintaiwan AT tingyuyen multiplelocusvariablenumbertandemrepeatanalysismlvaofmacrolidesusceptibleandresistantmycoplasmapneumoniaeinchildrenintaiwan AT luanyingchang multiplelocusvariablenumbertandemrepeatanalysismlvaofmacrolidesusceptibleandresistantmycoplasmapneumoniaeinchildrenintaiwan AT yijenliau multiplelocusvariablenumbertandemrepeatanalysismlvaofmacrolidesusceptibleandresistantmycoplasmapneumoniaeinchildrenintaiwan AT honghsingliu multiplelocusvariablenumbertandemrepeatanalysismlvaofmacrolidesusceptibleandresistantmycoplasmapneumoniaeinchildrenintaiwan AT liminhuang multiplelocusvariablenumbertandemrepeatanalysismlvaofmacrolidesusceptibleandresistantmycoplasmapneumoniaeinchildrenintaiwan |