Gut Microbial and Associated Metabolite Markers for Colorectal Cancer Diagnosis
Globally, colorectal cancer (CRC) is the second most common cause of mortality worldwide. Considerable evidence indicates that dysbiosis of the gut microbial community and its metabolite secretions play a fundamental role in advanced adenoma (ADA) and CRC development and progression. This study is a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/8/2037 |
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| author | Areej A. Alhhazmi Renad M. Alhamawi Reema M. Almisned Hanouf A. Almutairi Ahdab A. Jan Shahad M. Kurdi Yahya A. Almutawif Waleed Mohammed-Saeid |
| author_facet | Areej A. Alhhazmi Renad M. Alhamawi Reema M. Almisned Hanouf A. Almutairi Ahdab A. Jan Shahad M. Kurdi Yahya A. Almutawif Waleed Mohammed-Saeid |
| author_sort | Areej A. Alhhazmi |
| collection | DOAJ |
| description | Globally, colorectal cancer (CRC) is the second most common cause of mortality worldwide. Considerable evidence indicates that dysbiosis of the gut microbial community and its metabolite secretions play a fundamental role in advanced adenoma (ADA) and CRC development and progression. This study is a systematic review that aims to assess the clinical association between gut microbial markers and/or gut and circulating metabolites with ADA and CRC. Five electronic databases were searched by four independent reviewers. Only controlled trials that compared ADA and/or CRC with healthy control (HC) using either untargeted (16s rRNA gene or whole genome sequencing) or targeted (gene-based real-time PCR) identification methods for gut microbiome profile, or untargeted or targeted metabolite profiling approaches from the gut or serum/plasma, were eligible. Three independent reviewers evaluated the quality of the studies using the <i>Cochrane Handbook for Systematic Reviews of Interventions</i>. Twenty-four studies were eligible. We identified strong evidence of two microbial markers <i>Fusobacterium</i> and <i>Porphyromonas</i> for ADA vs. CRC, and nine microbial markers <i>Lachnospiraceae</i>-Lachnoclostridium, <i>Ruminococcaceae</i>-Ruminococcus, <i>Parvimonas</i> spp., <i>Parvimonas micra</i>, Enterobacteriaceae, <i>Fusobacterium</i> spp., Bacteroides, <i>Peptostreptococcus</i>-<i>Peptostreptococcus stomatis</i>, <i>Clostridia</i> spp.-<i>Clostridium hylemonae</i>, <i>Clostridium symbiosum</i>, and <i>Porphyromonas-Porphyromonas asaccharolytica</i> for CRC vs. HC. The remaining metabolite marker evidence between the various groups, including ADA vs. HC, ADA vs. HC, and CRC vs. HC, was not of sufficient quality to support additional findings. The identified gut microbial markers can be used in a panel for diagnosing ADA and/or CRC. Further research in the metabolite markers area is needed to evaluate the possibility to use in diagnostic or prognostic markers for colorectal cancer. |
| first_indexed | 2024-03-10T23:42:54Z |
| format | Article |
| id | doaj.art-a34bdab38f1148f38e2af9225eb52cb4 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T23:42:54Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a34bdab38f1148f38e2af9225eb52cb42023-11-19T02:18:08ZengMDPI AGMicroorganisms2076-26072023-08-01118203710.3390/microorganisms11082037Gut Microbial and Associated Metabolite Markers for Colorectal Cancer DiagnosisAreej A. Alhhazmi0Renad M. Alhamawi1Reema M. Almisned2Hanouf A. Almutairi3Ahdab A. Jan4Shahad M. Kurdi5Yahya A. Almutawif6Waleed Mohammed-Saeid7Medical Laboratories Technology Department, College of Applied Medical Sciences, Taibah University, P.O. Box 344, Al-Madinah Al-Munawarah 42353, Saudi ArabiaMedical Laboratories Technology Department, College of Applied Medical Sciences, Taibah University, P.O. Box 344, Al-Madinah Al-Munawarah 42353, Saudi ArabiaSeha Polyclinic, P.O. Box 150, Al-Madinah Al-Munawarah 41311, Saudi ArabiaBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), P.O. Box 6900, Thuwal 23955, Saudi ArabiaAbdulla Fouad Medical Supplies and Services (AFMS), P.O. Box 150, Al-Madinah Al-Munawarah 21414, Saudi ArabiaMedical Laboratories Technology Department, College of Applied Medical Sciences, Taibah University, P.O. Box 344, Al-Madinah Al-Munawarah 42353, Saudi ArabiaMedical Laboratories Technology Department, College of Applied Medical Sciences, Taibah University, P.O. Box 344, Al-Madinah Al-Munawarah 42353, Saudi ArabiaDepartment of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taibah University, P.O. Box 344, Al-Madinah Al-Munawarah 42353, Saudi ArabiaGlobally, colorectal cancer (CRC) is the second most common cause of mortality worldwide. Considerable evidence indicates that dysbiosis of the gut microbial community and its metabolite secretions play a fundamental role in advanced adenoma (ADA) and CRC development and progression. This study is a systematic review that aims to assess the clinical association between gut microbial markers and/or gut and circulating metabolites with ADA and CRC. Five electronic databases were searched by four independent reviewers. Only controlled trials that compared ADA and/or CRC with healthy control (HC) using either untargeted (16s rRNA gene or whole genome sequencing) or targeted (gene-based real-time PCR) identification methods for gut microbiome profile, or untargeted or targeted metabolite profiling approaches from the gut or serum/plasma, were eligible. Three independent reviewers evaluated the quality of the studies using the <i>Cochrane Handbook for Systematic Reviews of Interventions</i>. Twenty-four studies were eligible. We identified strong evidence of two microbial markers <i>Fusobacterium</i> and <i>Porphyromonas</i> for ADA vs. CRC, and nine microbial markers <i>Lachnospiraceae</i>-Lachnoclostridium, <i>Ruminococcaceae</i>-Ruminococcus, <i>Parvimonas</i> spp., <i>Parvimonas micra</i>, Enterobacteriaceae, <i>Fusobacterium</i> spp., Bacteroides, <i>Peptostreptococcus</i>-<i>Peptostreptococcus stomatis</i>, <i>Clostridia</i> spp.-<i>Clostridium hylemonae</i>, <i>Clostridium symbiosum</i>, and <i>Porphyromonas-Porphyromonas asaccharolytica</i> for CRC vs. HC. The remaining metabolite marker evidence between the various groups, including ADA vs. HC, ADA vs. HC, and CRC vs. HC, was not of sufficient quality to support additional findings. The identified gut microbial markers can be used in a panel for diagnosing ADA and/or CRC. Further research in the metabolite markers area is needed to evaluate the possibility to use in diagnostic or prognostic markers for colorectal cancer.https://www.mdpi.com/2076-2607/11/8/2037gut microbiotacolorectal cancermetabolites16s rRNA sequencereal-time PCRCRC |
| spellingShingle | Areej A. Alhhazmi Renad M. Alhamawi Reema M. Almisned Hanouf A. Almutairi Ahdab A. Jan Shahad M. Kurdi Yahya A. Almutawif Waleed Mohammed-Saeid Gut Microbial and Associated Metabolite Markers for Colorectal Cancer Diagnosis Microorganisms gut microbiota colorectal cancer metabolites 16s rRNA sequence real-time PCR CRC |
| title | Gut Microbial and Associated Metabolite Markers for Colorectal Cancer Diagnosis |
| title_full | Gut Microbial and Associated Metabolite Markers for Colorectal Cancer Diagnosis |
| title_fullStr | Gut Microbial and Associated Metabolite Markers for Colorectal Cancer Diagnosis |
| title_full_unstemmed | Gut Microbial and Associated Metabolite Markers for Colorectal Cancer Diagnosis |
| title_short | Gut Microbial and Associated Metabolite Markers for Colorectal Cancer Diagnosis |
| title_sort | gut microbial and associated metabolite markers for colorectal cancer diagnosis |
| topic | gut microbiota colorectal cancer metabolites 16s rRNA sequence real-time PCR CRC |
| url | https://www.mdpi.com/2076-2607/11/8/2037 |
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