Tuber melanosporum shapes nirS-type denitrifying and ammonia-oxidizing bacterial communities in Carya illinoinensis ectomycorrhizosphere soils
Background NirS-type denitrifying bacteria and ammonia-oxidizing bacteria (AOB) play a key role in the soil nitrogen cycle, which may affect the growth and development of underground truffles. We aimed to investigate nirS-type denitrifying bacterial and AOB community structures in the rhizosphere so...
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PeerJ Inc.
2020-09-01
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| author | Zongjing Kang Jie Zou Yue Huang Xiaoping Zhang Lei Ye Bo Zhang Xiaoping Zhang Xiaolin Li |
| author_facet | Zongjing Kang Jie Zou Yue Huang Xiaoping Zhang Lei Ye Bo Zhang Xiaoping Zhang Xiaolin Li |
| author_sort | Zongjing Kang |
| collection | DOAJ |
| description | Background NirS-type denitrifying bacteria and ammonia-oxidizing bacteria (AOB) play a key role in the soil nitrogen cycle, which may affect the growth and development of underground truffles. We aimed to investigate nirS-type denitrifying bacterial and AOB community structures in the rhizosphere soils of Carya illinoinensis seedlings inoculated with the black truffle (Tuber melanosporum) during the early symbiotic stage. Methods The C. illinoinensis seedlings inoculated with or without T. melanosporum were cultivated in a greenhouse for six months. Next-generation sequencing (NGS) technology was used to analyze nirS-type denitrifying bacterial and AOB community structures in the rhizosphere soils of these seedlings. Additionally, the soil properties were determined. Results The results indicated that the abundance and diversity of AOB were significantly reduced due to the inoculation of T. melanosporum, while these of nirS-type denitrifying bacteria increased significantly. Proteobacteria were the dominant bacterial groups, and Rhodanobacter, Pseudomonas, Nitrosospira and Nitrosomonas were the dominant classified bacterial genera in all the soil samples. Pseudomonas was the most abundant classified nirS-type denitrifying bacterial genus in ectomycorrhizosphere soils whose relative abundance could significantly increase after T. melanosporum inoculation. A large number of unclassified nirS-type denitrifying bacteria and AOB were observed. Moreover, T. melanosporum inoculation had little effect on the pH, total nitrogen (TN), nitrate-nitrogen (NO ${}_{3}^{-}$ 3 − -N) and ammonium-nitrogen (NH ${}_{4}^{+}$ 4 + -N) contents in ectomycorrhizosphere soils. Overall, our results showed that nirS-type denitrifying bacterial and AOB communities in C. illinoinensis rhizosphere soils were significantly affected by T. melanosporum on the initial stage of ectomycorrhizal symbiosis, without obvious variation of soil N contents. |
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| language | English |
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| spelling | doaj.art-a34c1e94386c4aa887f962338341981e2023-12-03T00:23:49ZengPeerJ Inc.PeerJ2167-83592020-09-018e945710.7717/peerj.9457Tuber melanosporum shapes nirS-type denitrifying and ammonia-oxidizing bacterial communities in Carya illinoinensis ectomycorrhizosphere soilsZongjing Kang0Jie Zou1Yue Huang2Xiaoping Zhang3Lei Ye4Bo Zhang5Xiaoping Zhang6Xiaolin Li7Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaDepartment of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaBackground NirS-type denitrifying bacteria and ammonia-oxidizing bacteria (AOB) play a key role in the soil nitrogen cycle, which may affect the growth and development of underground truffles. We aimed to investigate nirS-type denitrifying bacterial and AOB community structures in the rhizosphere soils of Carya illinoinensis seedlings inoculated with the black truffle (Tuber melanosporum) during the early symbiotic stage. Methods The C. illinoinensis seedlings inoculated with or without T. melanosporum were cultivated in a greenhouse for six months. Next-generation sequencing (NGS) technology was used to analyze nirS-type denitrifying bacterial and AOB community structures in the rhizosphere soils of these seedlings. Additionally, the soil properties were determined. Results The results indicated that the abundance and diversity of AOB were significantly reduced due to the inoculation of T. melanosporum, while these of nirS-type denitrifying bacteria increased significantly. Proteobacteria were the dominant bacterial groups, and Rhodanobacter, Pseudomonas, Nitrosospira and Nitrosomonas were the dominant classified bacterial genera in all the soil samples. Pseudomonas was the most abundant classified nirS-type denitrifying bacterial genus in ectomycorrhizosphere soils whose relative abundance could significantly increase after T. melanosporum inoculation. A large number of unclassified nirS-type denitrifying bacteria and AOB were observed. Moreover, T. melanosporum inoculation had little effect on the pH, total nitrogen (TN), nitrate-nitrogen (NO ${}_{3}^{-}$ 3 − -N) and ammonium-nitrogen (NH ${}_{4}^{+}$ 4 + -N) contents in ectomycorrhizosphere soils. Overall, our results showed that nirS-type denitrifying bacterial and AOB communities in C. illinoinensis rhizosphere soils were significantly affected by T. melanosporum on the initial stage of ectomycorrhizal symbiosis, without obvious variation of soil N contents.https://peerj.com/articles/9457.pdfTuber melanosporumCarya illinoinensisnirS-type denitrifying bacteriaAmmonia-oxidizing bacteriaEctomycorrhizosphere soils |
| spellingShingle | Zongjing Kang Jie Zou Yue Huang Xiaoping Zhang Lei Ye Bo Zhang Xiaoping Zhang Xiaolin Li Tuber melanosporum shapes nirS-type denitrifying and ammonia-oxidizing bacterial communities in Carya illinoinensis ectomycorrhizosphere soils PeerJ Tuber melanosporum Carya illinoinensis nirS-type denitrifying bacteria Ammonia-oxidizing bacteria Ectomycorrhizosphere soils |
| title | Tuber melanosporum shapes nirS-type denitrifying and ammonia-oxidizing bacterial communities in Carya illinoinensis ectomycorrhizosphere soils |
| title_full | Tuber melanosporum shapes nirS-type denitrifying and ammonia-oxidizing bacterial communities in Carya illinoinensis ectomycorrhizosphere soils |
| title_fullStr | Tuber melanosporum shapes nirS-type denitrifying and ammonia-oxidizing bacterial communities in Carya illinoinensis ectomycorrhizosphere soils |
| title_full_unstemmed | Tuber melanosporum shapes nirS-type denitrifying and ammonia-oxidizing bacterial communities in Carya illinoinensis ectomycorrhizosphere soils |
| title_short | Tuber melanosporum shapes nirS-type denitrifying and ammonia-oxidizing bacterial communities in Carya illinoinensis ectomycorrhizosphere soils |
| title_sort | tuber melanosporum shapes nirs type denitrifying and ammonia oxidizing bacterial communities in carya illinoinensis ectomycorrhizosphere soils |
| topic | Tuber melanosporum Carya illinoinensis nirS-type denitrifying bacteria Ammonia-oxidizing bacteria Ectomycorrhizosphere soils |
| url | https://peerj.com/articles/9457.pdf |
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