Comparative Proteomic Analysis of Hymenolepis diminuta Cysticercoid and Adult Stages
Cestodiases are common parasitic diseases of animals and humans. As cestodes have complex lifecycles, hexacanth larvae, metacestodes (including cysticercoids), and adults produce proteins allowing them to establish invasion and to survive in the hostile environment of the host. Hymenolepis diminuta...
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02672/full |
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| author | Anna Sulima Kirsi Savijoki Justyna Bień Anu Näreaho Rusłan Sałamatin Rusłan Sałamatin David Bruce Conn David Bruce Conn Daniel Młocicki Daniel Młocicki |
| author_facet | Anna Sulima Kirsi Savijoki Justyna Bień Anu Näreaho Rusłan Sałamatin Rusłan Sałamatin David Bruce Conn David Bruce Conn Daniel Młocicki Daniel Młocicki |
| author_sort | Anna Sulima |
| collection | DOAJ |
| description | Cestodiases are common parasitic diseases of animals and humans. As cestodes have complex lifecycles, hexacanth larvae, metacestodes (including cysticercoids), and adults produce proteins allowing them to establish invasion and to survive in the hostile environment of the host. Hymenolepis diminuta is the most commonly used model cestode in experimental parasitology. The aims of the present study were to perform a comparative proteomic analysis of two consecutive developmental stages of H. diminuta (cysticercoid and adult) and to distinguish proteins which might be characteristic for each of the stages from those shared by both stages. Somatic proteins of H. diminuta were isolated from 6-week-old cysticercoids and adult tapeworms. Cysticercoids were obtained from experimentally infected beetles, Tenebrio molitor, whereas adult worms were collected from experimentally infected rats. Proteins were separated by GeLC-MS/MS (one dimensional gel electrophoresis coupled with liquid chromatography and tandem mass spectrometry). Additionally protein samples were digested in-liquid and identified by LC-MS/MS. The identified proteins were classified according to molecular function, cellular components and biological processes. Our study showed a number of differences and similarities in the protein profiles of cysticercoids and adults; 233 cysticercoid and 182 adult proteins were identified. From these proteins, 131 were present only in the cysticercoid and 80 only in the adult stage samples. Both developmental stages shared 102 proteins; among which six represented immunomodulators and one is a potential drug target. In-liquid digestion and LC-MS/MS complemented and confirmed some of the GeLC-MS/MS identifications. Possible roles and functions of proteins identified with both proteomic approaches are discussed. |
| first_indexed | 2024-12-10T03:38:32Z |
| format | Article |
| id | doaj.art-a34c333cb8c14580a444c590988cd016 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T03:38:32Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a34c333cb8c14580a444c590988cd0162022-12-22T02:03:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-01-01810.3389/fmicb.2017.02672322968Comparative Proteomic Analysis of Hymenolepis diminuta Cysticercoid and Adult StagesAnna Sulima0Kirsi Savijoki1Justyna Bień2Anu Näreaho3Rusłan Sałamatin4Rusłan Sałamatin5David Bruce Conn6David Bruce Conn7Daniel Młocicki8Daniel Młocicki9Department of General Biology and Parasitology, Medical University of Warsaw, Warsaw, PolandDepartment of Food and Environmental Sciences, University of Helsinki, Helsinki, FinlandWitold Stefanski Institute of Parasitology, Polish Academy of Sciences, Warsaw, PolandDepartment of Veterinary Biosciences, University of Helsinki, Helsinki, FinlandDepartment of General Biology and Parasitology, Medical University of Warsaw, Warsaw, PolandDepartment of Parasitology and Vector-Borne Diseases, National Institute of Public Health—National Institute of Hygiene, Warsaw, PolandDepartment of Invertebrate Zoology, Museum of Comparative Zoology, Harvard University, Cambridge, MA, United StatesOne Health Center, Berry College, Mount Berry, GA, United StatesDepartment of General Biology and Parasitology, Medical University of Warsaw, Warsaw, PolandWitold Stefanski Institute of Parasitology, Polish Academy of Sciences, Warsaw, PolandCestodiases are common parasitic diseases of animals and humans. As cestodes have complex lifecycles, hexacanth larvae, metacestodes (including cysticercoids), and adults produce proteins allowing them to establish invasion and to survive in the hostile environment of the host. Hymenolepis diminuta is the most commonly used model cestode in experimental parasitology. The aims of the present study were to perform a comparative proteomic analysis of two consecutive developmental stages of H. diminuta (cysticercoid and adult) and to distinguish proteins which might be characteristic for each of the stages from those shared by both stages. Somatic proteins of H. diminuta were isolated from 6-week-old cysticercoids and adult tapeworms. Cysticercoids were obtained from experimentally infected beetles, Tenebrio molitor, whereas adult worms were collected from experimentally infected rats. Proteins were separated by GeLC-MS/MS (one dimensional gel electrophoresis coupled with liquid chromatography and tandem mass spectrometry). Additionally protein samples were digested in-liquid and identified by LC-MS/MS. The identified proteins were classified according to molecular function, cellular components and biological processes. Our study showed a number of differences and similarities in the protein profiles of cysticercoids and adults; 233 cysticercoid and 182 adult proteins were identified. From these proteins, 131 were present only in the cysticercoid and 80 only in the adult stage samples. Both developmental stages shared 102 proteins; among which six represented immunomodulators and one is a potential drug target. In-liquid digestion and LC-MS/MS complemented and confirmed some of the GeLC-MS/MS identifications. Possible roles and functions of proteins identified with both proteomic approaches are discussed.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02672/fullproteomic analysismass spectrometrytapewormsHymenolepis diminutahost–parasite interaction |
| spellingShingle | Anna Sulima Kirsi Savijoki Justyna Bień Anu Näreaho Rusłan Sałamatin Rusłan Sałamatin David Bruce Conn David Bruce Conn Daniel Młocicki Daniel Młocicki Comparative Proteomic Analysis of Hymenolepis diminuta Cysticercoid and Adult Stages Frontiers in Microbiology proteomic analysis mass spectrometry tapeworms Hymenolepis diminuta host–parasite interaction |
| title | Comparative Proteomic Analysis of Hymenolepis diminuta Cysticercoid and Adult Stages |
| title_full | Comparative Proteomic Analysis of Hymenolepis diminuta Cysticercoid and Adult Stages |
| title_fullStr | Comparative Proteomic Analysis of Hymenolepis diminuta Cysticercoid and Adult Stages |
| title_full_unstemmed | Comparative Proteomic Analysis of Hymenolepis diminuta Cysticercoid and Adult Stages |
| title_short | Comparative Proteomic Analysis of Hymenolepis diminuta Cysticercoid and Adult Stages |
| title_sort | comparative proteomic analysis of hymenolepis diminuta cysticercoid and adult stages |
| topic | proteomic analysis mass spectrometry tapeworms Hymenolepis diminuta host–parasite interaction |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02672/full |
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