Investigation of single strand DNA breaks in spontaneous abortion cases with known pesticide exposure
Pesticide exposure is an essential public health problem and endocrine-disrupting factor. Encounters during pregnancy may cause spontaneous abortions or congenital anomalies with embryotoxic or fetotoxic effects. DNA double-strand breaks occur upon exposure of DNA to radiation and chemicals or are c...
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| Format: | Article |
| Language: | English |
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Society of Turaz Bilim
2023-12-01
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| Series: | Medicine Science |
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| Online Access: | https://www.medicinescience.org/?mno=168601 |
| _version_ | 1797316539134771200 |
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| author | Jale Akgol Mine Kanat Pektas |
| author_facet | Jale Akgol Mine Kanat Pektas |
| author_sort | Jale Akgol |
| collection | DOAJ |
| description | Pesticide exposure is an essential public health problem and endocrine-disrupting factor. Encounters during pregnancy may cause spontaneous abortions or congenital anomalies with embryotoxic or fetotoxic effects. DNA double-strand breaks occur upon exposure of DNA to radiation and chemicals or are caused by faulty DNA metabolic processes. This research examines the relationship of DNA single-strand amount with pesticides and their types. Single-stranded DNA (ssDNA) density was quantitatively determined by fluorometric method in the blood of Spontaneous Abortion with organochlorine and or organophosphate pesticide exposure and in the blood of two patient groups who had normal delivery without pesticide contact. The mutation was screened by next-generation sequencing in 4 women who experienced spontaneous abortion with a high pesticide amount and variety. 91 female patients aged 18-35 years were included in the study. The spontaneous abortion group was 56 (61.5%), and the normal delivery group was 35 (38%) women. The mean age was 28.18(±4.5). There was a significant correlation between Pentachlorophenol -52 (PCB52) and ssDNA level (p0.05). It was determined that ssDNA levels were higher among those with multiple abortions. The ssDNA level was higher in those who had an abortion before (pA (p.Trp2502Ter) variation and the DYNC2H1 gene NM_001377.3 c.8458C>A (p.Pro2820Thr) variation associated with recurrent miscarriages were found to be heterozygous. Studies in this area need to be increased to determine the relationship of ssDNA elevation with pesticide exposure, its predictive value in abortion, and the value of environmental factors in repeated abortions whose etiology has not been clarified. [Med-Science 2023; 12(4.000): 1250-6] |
| first_indexed | 2024-03-08T03:19:51Z |
| format | Article |
| id | doaj.art-a351317f2ed9416bbbefd177a0472705 |
| institution | Directory Open Access Journal |
| issn | 2147-0634 |
| language | English |
| last_indexed | 2024-03-08T03:19:51Z |
| publishDate | 2023-12-01 |
| publisher | Society of Turaz Bilim |
| record_format | Article |
| series | Medicine Science |
| spelling | doaj.art-a351317f2ed9416bbbefd177a04727052024-02-12T10:32:08ZengSociety of Turaz BilimMedicine Science2147-06342023-12-011241250610.5455/medscience.2023.09.190168601Investigation of single strand DNA breaks in spontaneous abortion cases with known pesticide exposureJale Akgol0Mine Kanat Pektas1Afyonkarahisar Sağlık Bilimleri Universitesi Tıp Fakultesi B Blok Tıbbi Farmakoloji Anabilim Dalı/ AFYONKARAHİSAR Afyonkarahisar Sağlık Bilimleri Universitesi Tıp Fakultesi Hastanesi Kadın Hastalıkları ve Doğum Anabilim Dalı / AFYONKARAHİSARPesticide exposure is an essential public health problem and endocrine-disrupting factor. Encounters during pregnancy may cause spontaneous abortions or congenital anomalies with embryotoxic or fetotoxic effects. DNA double-strand breaks occur upon exposure of DNA to radiation and chemicals or are caused by faulty DNA metabolic processes. This research examines the relationship of DNA single-strand amount with pesticides and their types. Single-stranded DNA (ssDNA) density was quantitatively determined by fluorometric method in the blood of Spontaneous Abortion with organochlorine and or organophosphate pesticide exposure and in the blood of two patient groups who had normal delivery without pesticide contact. The mutation was screened by next-generation sequencing in 4 women who experienced spontaneous abortion with a high pesticide amount and variety. 91 female patients aged 18-35 years were included in the study. The spontaneous abortion group was 56 (61.5%), and the normal delivery group was 35 (38%) women. The mean age was 28.18(±4.5). There was a significant correlation between Pentachlorophenol -52 (PCB52) and ssDNA level (p0.05). It was determined that ssDNA levels were higher among those with multiple abortions. The ssDNA level was higher in those who had an abortion before (pA (p.Trp2502Ter) variation and the DYNC2H1 gene NM_001377.3 c.8458C>A (p.Pro2820Thr) variation associated with recurrent miscarriages were found to be heterozygous. Studies in this area need to be increased to determine the relationship of ssDNA elevation with pesticide exposure, its predictive value in abortion, and the value of environmental factors in repeated abortions whose etiology has not been clarified. [Med-Science 2023; 12(4.000): 1250-6]https://www.medicinescience.org/?mno=168601pesticidespontaneous abortionamount of ssdnagenotoxicitymutation |
| spellingShingle | Jale Akgol Mine Kanat Pektas Investigation of single strand DNA breaks in spontaneous abortion cases with known pesticide exposure Medicine Science pesticide spontaneous abortion amount of ssdna genotoxicity mutation |
| title | Investigation of single strand DNA breaks in spontaneous abortion cases with known pesticide exposure |
| title_full | Investigation of single strand DNA breaks in spontaneous abortion cases with known pesticide exposure |
| title_fullStr | Investigation of single strand DNA breaks in spontaneous abortion cases with known pesticide exposure |
| title_full_unstemmed | Investigation of single strand DNA breaks in spontaneous abortion cases with known pesticide exposure |
| title_short | Investigation of single strand DNA breaks in spontaneous abortion cases with known pesticide exposure |
| title_sort | investigation of single strand dna breaks in spontaneous abortion cases with known pesticide exposure |
| topic | pesticide spontaneous abortion amount of ssdna genotoxicity mutation |
| url | https://www.medicinescience.org/?mno=168601 |
| work_keys_str_mv | AT jaleakgol investigationofsinglestranddnabreaksinspontaneousabortioncaseswithknownpesticideexposure AT minekanatpektas investigationofsinglestranddnabreaksinspontaneousabortioncaseswithknownpesticideexposure |