Why RGB Imaging Should be Used to Analyze Fusarium Graminearum Growth and Estimate Deoxynivalenol Contamination
Size-based fungal growth studies are limited because they do not provide information about the mold’s state of maturity, and measurements such as radius and diameter are not practical if the fungus grows irregularly. Furthermore, the current methods used to detect diseases such as Fusarium...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Methods and Protocols |
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| Online Access: | http://www.mdpi.com/2409-9279/2/1/25 |
| _version_ | 1818959978375938048 |
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| author | Edgar Cambaza Shigenobu Koseki Shuso Kawamura |
| author_facet | Edgar Cambaza Shigenobu Koseki Shuso Kawamura |
| author_sort | Edgar Cambaza |
| collection | DOAJ |
| description | Size-based fungal growth studies are limited because they do not provide information about the mold’s state of maturity, and measurements such as radius and diameter are not practical if the fungus grows irregularly. Furthermore, the current methods used to detect diseases such as Fusarium head blight (FHB) or mycotoxin contamination are labor-intensive and time consuming. FHB is frequently detected through visual examination and the results can be subjective, depending on the skills and experience of the analyzer. For toxin determination (e.g., deoxynivalenol (DON), the best methods are expensive, not practical for routine. RGB (red, green and blue) imaging analysis is a viable alternative that is inexpensive, easy to use and seemingly better if enhanced with statistical methods. This short communication explains why RGB imaging analysis should be used instead of size-based variables as a tool to measure growth of Fusarium graminearum and DON concentration. |
| first_indexed | 2024-12-20T11:50:13Z |
| format | Article |
| id | doaj.art-a351f530f01a423da4fc7facf119bf80 |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-12-20T11:50:13Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-a351f530f01a423da4fc7facf119bf802022-12-21T19:41:47ZengMDPI AGMethods and Protocols2409-92792019-03-01212510.3390/mps2010025mps2010025Why RGB Imaging Should be Used to Analyze Fusarium Graminearum Growth and Estimate Deoxynivalenol ContaminationEdgar Cambaza0Shigenobu Koseki1Shuso Kawamura2Laboratory of Food Process Engineering, Graduate School of Agriculture, Hokkaido University, Sapporo 060-0808, JapanLaboratory of Food Process Engineering, Graduate School of Agriculture, Hokkaido University, Sapporo 060-0808, JapanLaboratory of Food Process Engineering, Graduate School of Agriculture, Hokkaido University, Sapporo 060-0808, JapanSize-based fungal growth studies are limited because they do not provide information about the mold’s state of maturity, and measurements such as radius and diameter are not practical if the fungus grows irregularly. Furthermore, the current methods used to detect diseases such as Fusarium head blight (FHB) or mycotoxin contamination are labor-intensive and time consuming. FHB is frequently detected through visual examination and the results can be subjective, depending on the skills and experience of the analyzer. For toxin determination (e.g., deoxynivalenol (DON), the best methods are expensive, not practical for routine. RGB (red, green and blue) imaging analysis is a viable alternative that is inexpensive, easy to use and seemingly better if enhanced with statistical methods. This short communication explains why RGB imaging analysis should be used instead of size-based variables as a tool to measure growth of Fusarium graminearum and DON concentration.http://www.mdpi.com/2409-9279/2/1/25RGBFusarium graminearumgrowthdeoxynivalenol |
| spellingShingle | Edgar Cambaza Shigenobu Koseki Shuso Kawamura Why RGB Imaging Should be Used to Analyze Fusarium Graminearum Growth and Estimate Deoxynivalenol Contamination Methods and Protocols RGB Fusarium graminearum growth deoxynivalenol |
| title | Why RGB Imaging Should be Used to Analyze Fusarium Graminearum Growth and Estimate Deoxynivalenol Contamination |
| title_full | Why RGB Imaging Should be Used to Analyze Fusarium Graminearum Growth and Estimate Deoxynivalenol Contamination |
| title_fullStr | Why RGB Imaging Should be Used to Analyze Fusarium Graminearum Growth and Estimate Deoxynivalenol Contamination |
| title_full_unstemmed | Why RGB Imaging Should be Used to Analyze Fusarium Graminearum Growth and Estimate Deoxynivalenol Contamination |
| title_short | Why RGB Imaging Should be Used to Analyze Fusarium Graminearum Growth and Estimate Deoxynivalenol Contamination |
| title_sort | why rgb imaging should be used to analyze fusarium graminearum growth and estimate deoxynivalenol contamination |
| topic | RGB Fusarium graminearum growth deoxynivalenol |
| url | http://www.mdpi.com/2409-9279/2/1/25 |
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