SGEF is a potential prognostic and therapeutic target for lung adenocarcinoma
Abstract Background SH3-containing guanine nucleotide exchange factor (SGEF), a RhoG-specific guanine nucleotide exchange factor (GEF), was consider as a key signal that determines cancer cell invasion. Although SGEF has been considered to highly express in glioma and prostate cancer. However, it is...
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12957-018-1331-8 |
| _version_ | 1831666161618518016 |
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| author | Qian Chen Xiao Lu Quan-Xing Liu Dong Zhou Yuan Qiu Ji-Gang Dai Hong Zheng |
| author_facet | Qian Chen Xiao Lu Quan-Xing Liu Dong Zhou Yuan Qiu Ji-Gang Dai Hong Zheng |
| author_sort | Qian Chen |
| collection | DOAJ |
| description | Abstract Background SH3-containing guanine nucleotide exchange factor (SGEF), a RhoG-specific guanine nucleotide exchange factor (GEF), was consider as a key signal that determines cancer cell invasion. Although SGEF has been considered to highly express in glioma and prostate cancer. However, it is not well illustrated in LAC. Methods In this experiment, expression of SGEF was detected in 92 LAC and corresponding normal tissue samples by immunohistochemistry. In addition, we evaluated the invasion and migration of lung adenocarcinoma cells by the gain and loss of SGEF expression. Furthermore, RhoG activity was measured by GST pull-down assay. Results SGEF is highly expressed in LAC tissues than in normal lung tissues and was associated with the TNM stage. Lung adenocarcinoma patients with low SGEF subgroup had longer overall survival compared to those with high expression. Furthermore, univariate analysis showed that SGEF expression was an independent prognostic factor for overall survival in lung adenocarcinoma. Silencing of SGEF effectively suppressed the invasion and migration of human lung adenocarcinoma cells in vitro by inhibiting RhoG activity, and over-expression of SGEF could reverse this phenomena. Conclusion SGEF is a novel prognostic target in human lung adenocarcinoma. |
| first_indexed | 2024-12-19T19:49:06Z |
| format | Article |
| id | doaj.art-a35498841ad041ad92b2c0122621d9e1 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-19T19:49:06Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-a35498841ad041ad92b2c0122621d9e12022-12-21T20:08:04ZengBMCWorld Journal of Surgical Oncology1477-78192018-02-011611710.1186/s12957-018-1331-8SGEF is a potential prognostic and therapeutic target for lung adenocarcinomaQian Chen0Xiao Lu1Quan-Xing Liu2Dong Zhou3Yuan Qiu4Ji-Gang Dai5Hong Zheng6Team four of the Second group, Graduate school, Third Military Medical University (Army medical university)Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University (Army medical university)Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University (Army medical university)Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University (Army medical university)Department of General Surgery, Xinqiao Hospital, Third Military Medical University (Army medical university)Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University (Army medical university)Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University (Army medical university)Abstract Background SH3-containing guanine nucleotide exchange factor (SGEF), a RhoG-specific guanine nucleotide exchange factor (GEF), was consider as a key signal that determines cancer cell invasion. Although SGEF has been considered to highly express in glioma and prostate cancer. However, it is not well illustrated in LAC. Methods In this experiment, expression of SGEF was detected in 92 LAC and corresponding normal tissue samples by immunohistochemistry. In addition, we evaluated the invasion and migration of lung adenocarcinoma cells by the gain and loss of SGEF expression. Furthermore, RhoG activity was measured by GST pull-down assay. Results SGEF is highly expressed in LAC tissues than in normal lung tissues and was associated with the TNM stage. Lung adenocarcinoma patients with low SGEF subgroup had longer overall survival compared to those with high expression. Furthermore, univariate analysis showed that SGEF expression was an independent prognostic factor for overall survival in lung adenocarcinoma. Silencing of SGEF effectively suppressed the invasion and migration of human lung adenocarcinoma cells in vitro by inhibiting RhoG activity, and over-expression of SGEF could reverse this phenomena. Conclusion SGEF is a novel prognostic target in human lung adenocarcinoma.http://link.springer.com/article/10.1186/s12957-018-1331-8Lung adenocarcinomaSGEFInvasionPoor prognosis |
| spellingShingle | Qian Chen Xiao Lu Quan-Xing Liu Dong Zhou Yuan Qiu Ji-Gang Dai Hong Zheng SGEF is a potential prognostic and therapeutic target for lung adenocarcinoma World Journal of Surgical Oncology Lung adenocarcinoma SGEF Invasion Poor prognosis |
| title | SGEF is a potential prognostic and therapeutic target for lung adenocarcinoma |
| title_full | SGEF is a potential prognostic and therapeutic target for lung adenocarcinoma |
| title_fullStr | SGEF is a potential prognostic and therapeutic target for lung adenocarcinoma |
| title_full_unstemmed | SGEF is a potential prognostic and therapeutic target for lung adenocarcinoma |
| title_short | SGEF is a potential prognostic and therapeutic target for lung adenocarcinoma |
| title_sort | sgef is a potential prognostic and therapeutic target for lung adenocarcinoma |
| topic | Lung adenocarcinoma SGEF Invasion Poor prognosis |
| url | http://link.springer.com/article/10.1186/s12957-018-1331-8 |
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