Identification and Expression of Inward-Rectifying Potassium Channel Subunits in <i>Plutella xylostella</i>
In insects, inward-rectifying potassium (Kir) channels regulate vital physiological functions, such as feeding behavior, silk secretion, renal excretion, and immune function. Therefore, they offer promising potential as targets for insecticides. Three types of Kir subunits have been identified in Di...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/11/8/461 |
| _version_ | 1797561662440472576 |
|---|---|
| author | Xiaoyi Lai Jie Xu Haihao Ma Zheming Liu Wei Zheng Jia Liu Hang Zhu Yong Zhou Xiaomao Zhou |
| author_facet | Xiaoyi Lai Jie Xu Haihao Ma Zheming Liu Wei Zheng Jia Liu Hang Zhu Yong Zhou Xiaomao Zhou |
| author_sort | Xiaoyi Lai |
| collection | DOAJ |
| description | In insects, inward-rectifying potassium (Kir) channels regulate vital physiological functions, such as feeding behavior, silk secretion, renal excretion, and immune function. Therefore, they offer promising potential as targets for insecticides. Three types of Kir subunits have been identified in Diptera and Hemiptera, but the Kir subunits of Lepidoptera still remain unclear. This study identified five Kir subunit genes (<i>pxkir1</i>, <i>pxkir2</i>, <i>pxkir3A</i>, <i>pxkir3B</i>, and <i>pxkir4</i>) in the transcriptome of <i>Plutella xylostella</i>. Phylogenetic analysis identified <i>pxkir1</i>, <i>pxkir2</i>, <i>pxkir3A</i>, and <i>pxkir3B</i> as orthologous genes of <i>kir1</i>–<i>3</i> in other insects. Interestingly, <i>pxkir4</i> may be encoding a new class of Kir subunit in Lepidoptera that has not been reported to date. To identify further Kir channel subunits of <i>P. xylostella</i>, the gene expression profiles of five <i>pxkir</i> genes were studied by quantitative real-time PCR. These <i>pxkir</i> genes are expressed throughout the development of <i>P. xylostella</i>. <i>pxkir1</i> and <i>pxkir2</i> were highly expressed in thoraxes and legs, while <i>pxkir3</i> (3A and 3B) and <i>pxkir4</i> had high expression levels in the midgut and Malpighian tubules. This study identified the composition and distribution of Kir subunits in <i>P. xylostella</i> for the first time, and provides useful information for the further study of Kir channel subunits in Lepidoptera. |
| first_indexed | 2024-03-10T18:17:10Z |
| format | Article |
| id | doaj.art-a3568370c1064a848631ae8bf404c91a |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T18:17:10Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-a3568370c1064a848631ae8bf404c91a2023-11-20T07:36:15ZengMDPI AGInsects2075-44502020-07-0111846110.3390/insects11080461Identification and Expression of Inward-Rectifying Potassium Channel Subunits in <i>Plutella xylostella</i>Xiaoyi Lai0Jie Xu1Haihao Ma2Zheming Liu3Wei Zheng4Jia Liu5Hang Zhu6Yong Zhou7Xiaomao Zhou8Long Ping Branch, Graduate School of Hunan University, Changsha 410125, ChinaInstitute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaInstitute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaInstitute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaLong Ping Branch, Graduate School of Hunan University, Changsha 410125, ChinaInstitute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaInstitute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaInstitute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaLong Ping Branch, Graduate School of Hunan University, Changsha 410125, ChinaIn insects, inward-rectifying potassium (Kir) channels regulate vital physiological functions, such as feeding behavior, silk secretion, renal excretion, and immune function. Therefore, they offer promising potential as targets for insecticides. Three types of Kir subunits have been identified in Diptera and Hemiptera, but the Kir subunits of Lepidoptera still remain unclear. This study identified five Kir subunit genes (<i>pxkir1</i>, <i>pxkir2</i>, <i>pxkir3A</i>, <i>pxkir3B</i>, and <i>pxkir4</i>) in the transcriptome of <i>Plutella xylostella</i>. Phylogenetic analysis identified <i>pxkir1</i>, <i>pxkir2</i>, <i>pxkir3A</i>, and <i>pxkir3B</i> as orthologous genes of <i>kir1</i>–<i>3</i> in other insects. Interestingly, <i>pxkir4</i> may be encoding a new class of Kir subunit in Lepidoptera that has not been reported to date. To identify further Kir channel subunits of <i>P. xylostella</i>, the gene expression profiles of five <i>pxkir</i> genes were studied by quantitative real-time PCR. These <i>pxkir</i> genes are expressed throughout the development of <i>P. xylostella</i>. <i>pxkir1</i> and <i>pxkir2</i> were highly expressed in thoraxes and legs, while <i>pxkir3</i> (3A and 3B) and <i>pxkir4</i> had high expression levels in the midgut and Malpighian tubules. This study identified the composition and distribution of Kir subunits in <i>P. xylostella</i> for the first time, and provides useful information for the further study of Kir channel subunits in Lepidoptera.https://www.mdpi.com/2075-4450/11/8/461<i>Plutella xylostella</i>Kir channelphylogenetic analysisreal-time PCR |
| spellingShingle | Xiaoyi Lai Jie Xu Haihao Ma Zheming Liu Wei Zheng Jia Liu Hang Zhu Yong Zhou Xiaomao Zhou Identification and Expression of Inward-Rectifying Potassium Channel Subunits in <i>Plutella xylostella</i> Insects <i>Plutella xylostella</i> Kir channel phylogenetic analysis real-time PCR |
| title | Identification and Expression of Inward-Rectifying Potassium Channel Subunits in <i>Plutella xylostella</i> |
| title_full | Identification and Expression of Inward-Rectifying Potassium Channel Subunits in <i>Plutella xylostella</i> |
| title_fullStr | Identification and Expression of Inward-Rectifying Potassium Channel Subunits in <i>Plutella xylostella</i> |
| title_full_unstemmed | Identification and Expression of Inward-Rectifying Potassium Channel Subunits in <i>Plutella xylostella</i> |
| title_short | Identification and Expression of Inward-Rectifying Potassium Channel Subunits in <i>Plutella xylostella</i> |
| title_sort | identification and expression of inward rectifying potassium channel subunits in i plutella xylostella i |
| topic | <i>Plutella xylostella</i> Kir channel phylogenetic analysis real-time PCR |
| url | https://www.mdpi.com/2075-4450/11/8/461 |
| work_keys_str_mv | AT xiaoyilai identificationandexpressionofinwardrectifyingpotassiumchannelsubunitsiniplutellaxylostellai AT jiexu identificationandexpressionofinwardrectifyingpotassiumchannelsubunitsiniplutellaxylostellai AT haihaoma identificationandexpressionofinwardrectifyingpotassiumchannelsubunitsiniplutellaxylostellai AT zhemingliu identificationandexpressionofinwardrectifyingpotassiumchannelsubunitsiniplutellaxylostellai AT weizheng identificationandexpressionofinwardrectifyingpotassiumchannelsubunitsiniplutellaxylostellai AT jialiu identificationandexpressionofinwardrectifyingpotassiumchannelsubunitsiniplutellaxylostellai AT hangzhu identificationandexpressionofinwardrectifyingpotassiumchannelsubunitsiniplutellaxylostellai AT yongzhou identificationandexpressionofinwardrectifyingpotassiumchannelsubunitsiniplutellaxylostellai AT xiaomaozhou identificationandexpressionofinwardrectifyingpotassiumchannelsubunitsiniplutellaxylostellai |