Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method
OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2010-12-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-35002 |
| _version_ | 1797916860989046784 |
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| author | Gülderen Yanıkkaya Demirel Tülin Budak-Alpdoğan Sema Aktaş Mahmut Bayık |
| author_facet | Gülderen Yanıkkaya Demirel Tülin Budak-Alpdoğan Sema Aktaş Mahmut Bayık |
| author_sort | Gülderen Yanıkkaya Demirel |
| collection | DOAJ |
| description | OBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies. |
| first_indexed | 2024-04-10T13:05:03Z |
| format | Article |
| id | doaj.art-a358be6ae833422b96d5f1988cd8e4fd |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T13:05:03Z |
| publishDate | 2010-12-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-a358be6ae833422b96d5f1988cd8e4fd2023-02-15T16:13:00ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632010-12-0127423424110.5152/tjh.2010.44TJH-35002Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution methodGülderen Yanıkkaya Demirel0Tülin Budak-Alpdoğan1Sema Aktaş2Mahmut Bayık3Stem Cell Laboratory Of Hematology Department, Yeditepe University, İstanbul, TurkeyDepartment Of Medicine, University Of Medicine And Dentistry Of New Jersey, New Jersey, UsaStem Cell Laboratory Of Hematology Department, Yeditepe University, İstanbul, TurkeyDepartment Of Internal Medicine, Marmara University, Hematology-ımmunology Section, İstanbulOBJECTIVE: Even though much progress has been made in defining primitive hematologic cell phenotypes by using flow cytometry and clonogenic methods, the direct method for study of marrow repopulating cells still remains to be elusive. Long Term Culture-Initiating Cells (LTC-IC) are known as the most primitive human hematopoietic cells detectable by in vitro functional assays. METHODS: In this study, LTC-IC with limiting dilution assay was used to evaluate repopulating potential of cord blood stem cells. RESULTS: CD34 selections from cord blood were completed succesfully with magnetic beads (73,64%+-9,12). The average incidence of week 5 LTC-IC was 1: 1966 CD34+ cells (range 1261–2906). CONCLUSION: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells. This may be due to the lack of some transcription and growth factors along with some cytokines and chemokines released by accessory cells which are necessary for proliferation of cord blood progenitor/stem cells and it presents an area of interest for further studies.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-35002stem cellcord bloodltc-iclimiting dilutioncfu assaycytometry |
| spellingShingle | Gülderen Yanıkkaya Demirel Tülin Budak-Alpdoğan Sema Aktaş Mahmut Bayık Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method Turkish Journal of Hematology stem cell cord blood ltc-ic limiting dilution cfu assay cytometry |
| title | Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method |
| title_full | Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method |
| title_fullStr | Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method |
| title_full_unstemmed | Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method |
| title_short | Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method |
| title_sort | long term culture initiating cells ltc ic produced from cd34 cord blood cells with limiting dilution method |
| topic | stem cell cord blood ltc-ic limiting dilution cfu assay cytometry |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-35002 |
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