miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer
Growing evidence points at an association between microRNAs and tumor development. Although dysregulation of microRNA-103a-3p (miR-103a-3p) in multiple human cancers has been reported, its expression in prostate cancer (PCa) remains unknown and there is currently no research on the relationship betw...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-08-01
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| Series: | Journal of Investigative Surgery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08941939.2020.1738602 |
| _version_ | 1797684621201113088 |
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| author | Jiyue Ge Likai Mao Weiqiang Xu Wenge Fang Ningning Wang Dawen Ye Zhuang Dong Han Guan Chao Guan |
| author_facet | Jiyue Ge Likai Mao Weiqiang Xu Wenge Fang Ningning Wang Dawen Ye Zhuang Dong Han Guan Chao Guan |
| author_sort | Jiyue Ge |
| collection | DOAJ |
| description | Growing evidence points at an association between microRNAs and tumor development. Although dysregulation of microRNA-103a-3p (miR-103a-3p) in multiple human cancers has been reported, its expression in prostate cancer (PCa) remains unknown and there is currently no research on the relationship between miR-103a-3p and tumor protein D52 (TPD52) in PCa. Our aim in this study was to explore the effect and potential mechanism of miR-103a-3p in PCa. qRT-PCR was performed to detected the level of miR-103a-3p in PCa tissues and cells, and in normal tissues. Colony, wound-healing, invasion, proliferation, and apoptosis assays were performed in search miR-103a-3p effect in PCa. TargetScan was used to predict potential targets of miR-103a-3p. Additionally, dual-luciferase reporter, western blot, and immunofluorescence assays were performed to detected the target gene of miR-103a-3p. Finally, we explore the differences in tumor xenograft experiments between nude mice injected with stably miR-103a-3p expressing cells and those expressing a miR-negative control. Low level of miR-103a-3p was detected in PCa tissues and cells, when compared with normal tissues. Enhancement of miR-103a-3p significantly inhibited migration and invasion of PCa cells, and negatively regulated expression of the oncogenic tumor protein D52 (TPD52) through direct binding to its 3’-UTR. Interestingly, overexpression of TPD52 significantly attenuated the effect of mir-103a-3p on PCa. Our study provides the first evidence that miR-103a-3p directly targets TPD52 and inhibits the proliferation and invasion of PCa. This finding helps clarify the role of mir-103a-3p-TPD52 axis in PCa and may provide new therapeutic targets for the disease. |
| first_indexed | 2024-03-12T00:33:19Z |
| format | Article |
| id | doaj.art-a358e5782baf4bc9ae0d4869ef776d92 |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:33:19Z |
| publishDate | 2021-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-a358e5782baf4bc9ae0d4869ef776d922023-09-15T10:07:32ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532021-08-0134998499210.1080/08941939.2020.17386021738602miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate CancerJiyue Ge0Likai Mao1Weiqiang Xu2Wenge Fang3Ningning Wang4Dawen Ye5Zhuang Dong6Han Guan7Chao Guan8Department of Urology, The Second Affiliated Hospital of Bengbu Medical CollegeDepartment of Urology, The Second Affiliated Hospital of Bengbu Medical CollegeDepartment of Urology, The Second Affiliated Hospital of Bengbu Medical CollegeDepartment of Urology, The Second Affiliated Hospital of Bengbu Medical CollegeDepartment of Urology, The First Affiliated Hospital of Bengbu Medical CollegeDepartment of Urology, The Second Affiliated Hospital of Bengbu Medical CollegeDepartment of Urology, The Second Affiliated Hospital of Bengbu Medical CollegeDepartment of Urology, The First Affiliated Hospital of Bengbu Medical CollegeDepartment of Urology, The Second Affiliated Hospital of Bengbu Medical CollegeGrowing evidence points at an association between microRNAs and tumor development. Although dysregulation of microRNA-103a-3p (miR-103a-3p) in multiple human cancers has been reported, its expression in prostate cancer (PCa) remains unknown and there is currently no research on the relationship between miR-103a-3p and tumor protein D52 (TPD52) in PCa. Our aim in this study was to explore the effect and potential mechanism of miR-103a-3p in PCa. qRT-PCR was performed to detected the level of miR-103a-3p in PCa tissues and cells, and in normal tissues. Colony, wound-healing, invasion, proliferation, and apoptosis assays were performed in search miR-103a-3p effect in PCa. TargetScan was used to predict potential targets of miR-103a-3p. Additionally, dual-luciferase reporter, western blot, and immunofluorescence assays were performed to detected the target gene of miR-103a-3p. Finally, we explore the differences in tumor xenograft experiments between nude mice injected with stably miR-103a-3p expressing cells and those expressing a miR-negative control. Low level of miR-103a-3p was detected in PCa tissues and cells, when compared with normal tissues. Enhancement of miR-103a-3p significantly inhibited migration and invasion of PCa cells, and negatively regulated expression of the oncogenic tumor protein D52 (TPD52) through direct binding to its 3’-UTR. Interestingly, overexpression of TPD52 significantly attenuated the effect of mir-103a-3p on PCa. Our study provides the first evidence that miR-103a-3p directly targets TPD52 and inhibits the proliferation and invasion of PCa. This finding helps clarify the role of mir-103a-3p-TPD52 axis in PCa and may provide new therapeutic targets for the disease.http://dx.doi.org/10.1080/08941939.2020.1738602mir-103a-3pproliferationinvasiontumor protein d52prostate cancer |
| spellingShingle | Jiyue Ge Likai Mao Weiqiang Xu Wenge Fang Ningning Wang Dawen Ye Zhuang Dong Han Guan Chao Guan miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer Journal of Investigative Surgery mir-103a-3p proliferation invasion tumor protein d52 prostate cancer |
| title | miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer |
| title_full | miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer |
| title_fullStr | miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer |
| title_full_unstemmed | miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer |
| title_short | miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer |
| title_sort | mir 103a 3p suppresses cell proliferation and invasion by targeting tumor protein d52 in prostate cancer |
| topic | mir-103a-3p proliferation invasion tumor protein d52 prostate cancer |
| url | http://dx.doi.org/10.1080/08941939.2020.1738602 |
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