Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys.
Recent physiological studies in the kidney proposed the existence of a secondary feedback mechanism termed 'crosstalk' localized after the macula densa. This newly discovered crosstalk contact between the nephron tubule and its own afferent arteriole may potentially revolutionize our under...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3764123?pdf=render |
| _version_ | 1818944304381427712 |
|---|---|
| author | Hao Ren Ning-Yu Liu Arne Andreasen Jesper S Thomsen Liu Cao Erik I Christensen Xiao-Yue Zhai |
| author_facet | Hao Ren Ning-Yu Liu Arne Andreasen Jesper S Thomsen Liu Cao Erik I Christensen Xiao-Yue Zhai |
| author_sort | Hao Ren |
| collection | DOAJ |
| description | Recent physiological studies in the kidney proposed the existence of a secondary feedback mechanism termed 'crosstalk' localized after the macula densa. This newly discovered crosstalk contact between the nephron tubule and its own afferent arteriole may potentially revolutionize our understanding of renal vascular resistance and electrolyte regulation. However, the nature of such a crosstalk mechanism is still debated due to a lack of direct and comprehensive morphological evidence. Its exact location along the nephron, its prevalence among the different types of nephrons, and the type of cells involved are yet unknown. To address these issues, computer assisted 3-dimensional nephron tracing was applied in combination with direct immunohistochemistry on plastic sections and electron microscopy. 'Random' contacts in the cortex were identified by the tracing and excluded. We investigated a total of 168 nephrons from all cortical regions. The results demonstrated that the crosstalk contact existed, and that it was only present in certain nephrons (90% of the short-looped and 75% of the long-looped nephrons). The crosstalk contacts always occurred at a specific position--the last 10% of the distal convoluted tubule. Importantly, we demonstrated, for the first time, that the cells found in the tubule wall at the contact site were always type nonA-nonB intercalated cells. In conclusion, the present work confirmed the existence of a post macula densa physical crosstalk contact. |
| first_indexed | 2024-12-20T07:41:06Z |
| format | Article |
| id | doaj.art-a35e172eb36b45d5b9fa01ad4e4b04ab |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T07:41:06Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a35e172eb36b45d5b9fa01ad4e4b04ab2022-12-21T19:48:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7089810.1371/journal.pone.0070898Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys.Hao RenNing-Yu LiuArne AndreasenJesper S ThomsenLiu CaoErik I ChristensenXiao-Yue ZhaiRecent physiological studies in the kidney proposed the existence of a secondary feedback mechanism termed 'crosstalk' localized after the macula densa. This newly discovered crosstalk contact between the nephron tubule and its own afferent arteriole may potentially revolutionize our understanding of renal vascular resistance and electrolyte regulation. However, the nature of such a crosstalk mechanism is still debated due to a lack of direct and comprehensive morphological evidence. Its exact location along the nephron, its prevalence among the different types of nephrons, and the type of cells involved are yet unknown. To address these issues, computer assisted 3-dimensional nephron tracing was applied in combination with direct immunohistochemistry on plastic sections and electron microscopy. 'Random' contacts in the cortex were identified by the tracing and excluded. We investigated a total of 168 nephrons from all cortical regions. The results demonstrated that the crosstalk contact existed, and that it was only present in certain nephrons (90% of the short-looped and 75% of the long-looped nephrons). The crosstalk contacts always occurred at a specific position--the last 10% of the distal convoluted tubule. Importantly, we demonstrated, for the first time, that the cells found in the tubule wall at the contact site were always type nonA-nonB intercalated cells. In conclusion, the present work confirmed the existence of a post macula densa physical crosstalk contact.http://europepmc.org/articles/PMC3764123?pdf=render |
| spellingShingle | Hao Ren Ning-Yu Liu Arne Andreasen Jesper S Thomsen Liu Cao Erik I Christensen Xiao-Yue Zhai Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys. PLoS ONE |
| title | Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys. |
| title_full | Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys. |
| title_fullStr | Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys. |
| title_full_unstemmed | Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys. |
| title_short | Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys. |
| title_sort | direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys |
| url | http://europepmc.org/articles/PMC3764123?pdf=render |
| work_keys_str_mv | AT haoren directphysicalcontactbetweenintercalatedcellsinthedistalconvolutedtubuleandtheafferentarterioleinmousekidneys AT ningyuliu directphysicalcontactbetweenintercalatedcellsinthedistalconvolutedtubuleandtheafferentarterioleinmousekidneys AT arneandreasen directphysicalcontactbetweenintercalatedcellsinthedistalconvolutedtubuleandtheafferentarterioleinmousekidneys AT jespersthomsen directphysicalcontactbetweenintercalatedcellsinthedistalconvolutedtubuleandtheafferentarterioleinmousekidneys AT liucao directphysicalcontactbetweenintercalatedcellsinthedistalconvolutedtubuleandtheafferentarterioleinmousekidneys AT erikichristensen directphysicalcontactbetweenintercalatedcellsinthedistalconvolutedtubuleandtheafferentarterioleinmousekidneys AT xiaoyuezhai directphysicalcontactbetweenintercalatedcellsinthedistalconvolutedtubuleandtheafferentarterioleinmousekidneys |