Development of inducer-free expression plasmids using IPTG-inducible Pspac promoter for Bacillus subtilis
An inducer-free expression vector for the low expression levels in Bacillus subtilis (B. subtilis) is necessary for fundamental research. In this study, we constructed inducer-free expression plasmids carrying Pspac, a well-known IPTG-inducible promoter, by removing a part of the lacI gene. Then, w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Vietnam Ministry of Science and Technology
2021-03-01
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| Series: | Vietnam Journal of Science, Technology and Engineering |
| Subjects: | |
| Online Access: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/22 |