An Engineered Nanocomplex with Photodynamic and Photothermal Synergistic Properties for Cancer Treatment
Photodynamic therapy (PDT) and photothermal therapy (PTT) are promising therapeutic methods for cancer treatment; however, as single modality therapies, either PDT or PTT is still limited in its success rate. A dual application of both PDT and PTT, in a combined protocol, has gained immense interest...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/4/2286 |
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| author | Eli Varon Gaddi Blumrosen Moshe Sinvani Elina Haimov Shlomi Polani Michal Natan Irit Shoval Avi Jacob Ayelet Atkins David Zitoun Orit Shefi |
| author_facet | Eli Varon Gaddi Blumrosen Moshe Sinvani Elina Haimov Shlomi Polani Michal Natan Irit Shoval Avi Jacob Ayelet Atkins David Zitoun Orit Shefi |
| author_sort | Eli Varon |
| collection | DOAJ |
| description | Photodynamic therapy (PDT) and photothermal therapy (PTT) are promising therapeutic methods for cancer treatment; however, as single modality therapies, either PDT or PTT is still limited in its success rate. A dual application of both PDT and PTT, in a combined protocol, has gained immense interest. In this study, gold nanoparticles (AuNPs) were conjugated with a PDT agent, meso-tetrahydroxyphenylchlorin (mTHPC) photosensitizer, designed as nanotherapeutic agents that can activate a dual photodynamic/photothermal therapy in SH-SY5Y human neuroblastoma cells. The AuNP-mTHPC complex is biocompatible, soluble, and photostable. PDT efficiency is high because of immediate reactive oxygen species (ROS) production upon mTHPC activation by the 650-nm laser, which decreased mitochondrial membrane potential (<sup>∆</sup>ψ<sub>m</sub>). Likewise, the AuNP-mTHPC complex is used as a photoabsorbing (PTA) agent for PTT, due to efficient plasmon absorption and excellent photothermal conversion characteristics of AuNPs under laser irradiation at 532 nm. Under the laser irradiation of a PDT/PTT combination, a twofold phototoxicity outcome follows, compared to PDT-only or PTT-only treatment. This indicates that PDT and PTT have synergistic effects together as a combined therapeutic method. Our study aimed at applying the AuNP-mTHPC approach as a potential treatment of cancer in the biomedical field. |
| first_indexed | 2024-03-09T21:44:15Z |
| format | Article |
| id | doaj.art-a3650ba856f9403aaaf733fffb4ca1e7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:44:15Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a3650ba856f9403aaaf733fffb4ca1e72023-11-23T20:22:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01234228610.3390/ijms23042286An Engineered Nanocomplex with Photodynamic and Photothermal Synergistic Properties for Cancer TreatmentEli Varon0Gaddi Blumrosen1Moshe Sinvani2Elina Haimov3Shlomi Polani4Michal Natan5Irit Shoval6Avi Jacob7Ayelet Atkins8David Zitoun9Orit Shefi10Faculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, IsraelBar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, IsraelFaculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, IsraelFaculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, IsraelBar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelBar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, IsraelPhotodynamic therapy (PDT) and photothermal therapy (PTT) are promising therapeutic methods for cancer treatment; however, as single modality therapies, either PDT or PTT is still limited in its success rate. A dual application of both PDT and PTT, in a combined protocol, has gained immense interest. In this study, gold nanoparticles (AuNPs) were conjugated with a PDT agent, meso-tetrahydroxyphenylchlorin (mTHPC) photosensitizer, designed as nanotherapeutic agents that can activate a dual photodynamic/photothermal therapy in SH-SY5Y human neuroblastoma cells. The AuNP-mTHPC complex is biocompatible, soluble, and photostable. PDT efficiency is high because of immediate reactive oxygen species (ROS) production upon mTHPC activation by the 650-nm laser, which decreased mitochondrial membrane potential (<sup>∆</sup>ψ<sub>m</sub>). Likewise, the AuNP-mTHPC complex is used as a photoabsorbing (PTA) agent for PTT, due to efficient plasmon absorption and excellent photothermal conversion characteristics of AuNPs under laser irradiation at 532 nm. Under the laser irradiation of a PDT/PTT combination, a twofold phototoxicity outcome follows, compared to PDT-only or PTT-only treatment. This indicates that PDT and PTT have synergistic effects together as a combined therapeutic method. Our study aimed at applying the AuNP-mTHPC approach as a potential treatment of cancer in the biomedical field.https://www.mdpi.com/1422-0067/23/4/2286engineeringbiomaterialsphototherapycancernanoparticles |
| spellingShingle | Eli Varon Gaddi Blumrosen Moshe Sinvani Elina Haimov Shlomi Polani Michal Natan Irit Shoval Avi Jacob Ayelet Atkins David Zitoun Orit Shefi An Engineered Nanocomplex with Photodynamic and Photothermal Synergistic Properties for Cancer Treatment International Journal of Molecular Sciences engineering biomaterials phototherapy cancer nanoparticles |
| title | An Engineered Nanocomplex with Photodynamic and Photothermal Synergistic Properties for Cancer Treatment |
| title_full | An Engineered Nanocomplex with Photodynamic and Photothermal Synergistic Properties for Cancer Treatment |
| title_fullStr | An Engineered Nanocomplex with Photodynamic and Photothermal Synergistic Properties for Cancer Treatment |
| title_full_unstemmed | An Engineered Nanocomplex with Photodynamic and Photothermal Synergistic Properties for Cancer Treatment |
| title_short | An Engineered Nanocomplex with Photodynamic and Photothermal Synergistic Properties for Cancer Treatment |
| title_sort | engineered nanocomplex with photodynamic and photothermal synergistic properties for cancer treatment |
| topic | engineering biomaterials phototherapy cancer nanoparticles |
| url | https://www.mdpi.com/1422-0067/23/4/2286 |
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