Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense
Plant cell wall is a complex and changeable structure, which is very important for plant growth and development. It is clear that cell wall polysaccharide synthases have critical functions in rice growth and abiotic stress, yet their role in plant response to pathogen invasion is poorly understood....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.980424/full |
| _version_ | 1811258668864241664 |
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| author | Xiong Liu Xiong Liu Zhongliang Yin Zhongliang Yin Yubo Wang Yubo Wang Sai Cao Sai Cao Wei Yao Jinling Liu Xuedan Lu Xuedan Lu Feng Wang Feng Wang Guilian Zhang Guilian Zhang Yunhua Xiao Yunhua Xiao Wenbang Tang Wenbang Tang Wenbang Tang Wenbang Tang Huabing Deng Huabing Deng |
| author_facet | Xiong Liu Xiong Liu Zhongliang Yin Zhongliang Yin Yubo Wang Yubo Wang Sai Cao Sai Cao Wei Yao Jinling Liu Xuedan Lu Xuedan Lu Feng Wang Feng Wang Guilian Zhang Guilian Zhang Yunhua Xiao Yunhua Xiao Wenbang Tang Wenbang Tang Wenbang Tang Wenbang Tang Huabing Deng Huabing Deng |
| author_sort | Xiong Liu |
| collection | DOAJ |
| description | Plant cell wall is a complex and changeable structure, which is very important for plant growth and development. It is clear that cell wall polysaccharide synthases have critical functions in rice growth and abiotic stress, yet their role in plant response to pathogen invasion is poorly understood. Here, we describe a dwarf and narrowed leaf in Hejiang 19 (dnl19) mutant in rice, which shows multiple growth defects such as reduced plant height, enlarged lamina joint angle, curled leaf morphology, and a decrease in panicle length and seed setting. MutMap analysis, genetic complementation and gene knockout mutant show that cellulose synthase-like D4 (OsCSLD4) is the causal gene for DNL19. Loss function of OsCSLD4 leads to a constitutive activation of defense response in rice. After inoculation with rice blast and bacterial blight, dnl19 displays an enhanced disease resistance. Widely targeted metabolomics analysis reveals that disruption of OsCSLD4 in dnl19 resulted in significant increase of L-valine, L-asparagine, L-histidine, L-alanine, gentisic acid, but significant decrease of L-aspartic acid, malic acid, 6-phosphogluconic acid, glucose 6-phosphate, galactose 1-phosphate, gluconic acid, D-aspartic acid. Collectively, our data reveals the importance of OsCSLD4 in balancing the trade-off between rice growth and defense. |
| first_indexed | 2024-04-12T18:16:59Z |
| format | Article |
| id | doaj.art-a36624a442fa49069eeb7412cc1700f0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T18:16:59Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a36624a442fa49069eeb7412cc1700f02022-12-22T03:21:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.980424980424Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defenseXiong Liu0Xiong Liu1Zhongliang Yin2Zhongliang Yin3Yubo Wang4Yubo Wang5Sai Cao6Sai Cao7Wei Yao8Jinling Liu9Xuedan Lu10Xuedan Lu11Feng Wang12Feng Wang13Guilian Zhang14Guilian Zhang15Yunhua Xiao16Yunhua Xiao17Wenbang Tang18Wenbang Tang19Wenbang Tang20Wenbang Tang21Huabing Deng22Huabing Deng23College of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaHunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, ChinaState Key Laboratory of Hybrid Rice, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, ChinaPlant cell wall is a complex and changeable structure, which is very important for plant growth and development. It is clear that cell wall polysaccharide synthases have critical functions in rice growth and abiotic stress, yet their role in plant response to pathogen invasion is poorly understood. Here, we describe a dwarf and narrowed leaf in Hejiang 19 (dnl19) mutant in rice, which shows multiple growth defects such as reduced plant height, enlarged lamina joint angle, curled leaf morphology, and a decrease in panicle length and seed setting. MutMap analysis, genetic complementation and gene knockout mutant show that cellulose synthase-like D4 (OsCSLD4) is the causal gene for DNL19. Loss function of OsCSLD4 leads to a constitutive activation of defense response in rice. After inoculation with rice blast and bacterial blight, dnl19 displays an enhanced disease resistance. Widely targeted metabolomics analysis reveals that disruption of OsCSLD4 in dnl19 resulted in significant increase of L-valine, L-asparagine, L-histidine, L-alanine, gentisic acid, but significant decrease of L-aspartic acid, malic acid, 6-phosphogluconic acid, glucose 6-phosphate, galactose 1-phosphate, gluconic acid, D-aspartic acid. Collectively, our data reveals the importance of OsCSLD4 in balancing the trade-off between rice growth and defense.https://www.frontiersin.org/articles/10.3389/fpls.2022.980424/fullricecellulose synthase-like Dtrade-offdisease resistancewidely targeted metabolomics |
| spellingShingle | Xiong Liu Xiong Liu Zhongliang Yin Zhongliang Yin Yubo Wang Yubo Wang Sai Cao Sai Cao Wei Yao Jinling Liu Xuedan Lu Xuedan Lu Feng Wang Feng Wang Guilian Zhang Guilian Zhang Yunhua Xiao Yunhua Xiao Wenbang Tang Wenbang Tang Wenbang Tang Wenbang Tang Huabing Deng Huabing Deng Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense Frontiers in Plant Science rice cellulose synthase-like D trade-off disease resistance widely targeted metabolomics |
| title | Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense |
| title_full | Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense |
| title_fullStr | Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense |
| title_full_unstemmed | Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense |
| title_short | Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense |
| title_sort | rice cellulose synthase like protein oscsld4 coordinates the trade off between plant growth and defense |
| topic | rice cellulose synthase-like D trade-off disease resistance widely targeted metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.980424/full |
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