The Effects of Physiological and Methodological Determinants on F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model
Introduction: In this study, the influence of physiological determinants on 18F-fluoro- d -glucose ( 18 F-FDG) brain uptake was evaluated in a mouse model of Alzheimer disease. Materials and Methods: TASTPM (Tg) and age-matched C57BL/6 J (WT) mice were fasted for 10 hours, while another group was fa...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2016-02-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012115624919 |
| _version_ | 1797285548569657344 |
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| author | Steven Deleye MSc Ann-Marie Waldron MSc Jill C. Richardson PhD Mark Schmidt PhD Xavier Langlois PhD Sigrid Stroobants MD, PhD Steven Staelens PhD |
| author_facet | Steven Deleye MSc Ann-Marie Waldron MSc Jill C. Richardson PhD Mark Schmidt PhD Xavier Langlois PhD Sigrid Stroobants MD, PhD Steven Staelens PhD |
| author_sort | Steven Deleye MSc |
| collection | DOAJ |
| description | Introduction: In this study, the influence of physiological determinants on 18F-fluoro- d -glucose ( 18 F-FDG) brain uptake was evaluated in a mouse model of Alzheimer disease. Materials and Methods: TASTPM (Tg) and age-matched C57BL/6 J (WT) mice were fasted for 10 hours, while another group was fasted for 20 hours to evaluate the effect of fasting duration. The effect of repeatedly scanning was evaluated by scanning Tg and WT mice at days 1, 4, and 7. Brain 18 F-FDG uptake was evaluated in the thalamus being the most indicative region. Finally, the cerebellum was tested as a reference region for the relative standard uptake value (rSUV). Results: When correcting the brain uptake for glucose, the effect of different fasting durations was attenuated and the anticipated hypometabolism in Tg mice was demonstrated. Also, with repeated scanning, the brain uptake values within a group and the hypometabolism of the Tg mice only remained stable over time when glucose correction was applied. Finally, hypometabolism was also observed in the cerebellum, yielding artificially higher rSUV values for Tg mice. Conclusion: Corrections for blood glucose levels have to be applied when semiquantifying 18 F-FDG brain uptake in mouse models for AD. Potential reference regions for normalization should be thoroughly investigated to ensure that they are not pathologically affected also by afferent connections. |
| first_indexed | 2024-03-07T18:04:49Z |
| format | Article |
| id | doaj.art-a367047fc0644842a2d1c1a2ee15907f |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T18:04:49Z |
| publishDate | 2016-02-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-a367047fc0644842a2d1c1a2ee15907f2024-03-02T09:53:05ZengSAGE PublicationsMolecular Imaging1536-01212016-02-011510.1177/153601211562491910.1177_1536012115624919The Effects of Physiological and Methodological Determinants on F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer ModelSteven Deleye MSc0Ann-Marie Waldron MSc1Jill C. Richardson PhD2Mark Schmidt PhD3Xavier Langlois PhD4Sigrid Stroobants MD, PhD5Steven Staelens PhD6 Molecular Imaging Center Antwerp, University of Antwerp, Antwerp, Belgium Molecular Imaging Center Antwerp, University of Antwerp, Antwerp, Belgium R&D China UK Group, GlaxoSmithKline, Stevenage, United Kingdom Neuroscience Department, Janssen Pharmaceutica NV, Beerse, Belgium Neuroscience Department, Janssen Pharmaceutica NV, Beerse, Belgium Nuclear Medicine Department, University Hospital Antwerp, Antwerp, Belgium Molecular Imaging Center Antwerp, University of Antwerp, Antwerp, BelgiumIntroduction: In this study, the influence of physiological determinants on 18F-fluoro- d -glucose ( 18 F-FDG) brain uptake was evaluated in a mouse model of Alzheimer disease. Materials and Methods: TASTPM (Tg) and age-matched C57BL/6 J (WT) mice were fasted for 10 hours, while another group was fasted for 20 hours to evaluate the effect of fasting duration. The effect of repeatedly scanning was evaluated by scanning Tg and WT mice at days 1, 4, and 7. Brain 18 F-FDG uptake was evaluated in the thalamus being the most indicative region. Finally, the cerebellum was tested as a reference region for the relative standard uptake value (rSUV). Results: When correcting the brain uptake for glucose, the effect of different fasting durations was attenuated and the anticipated hypometabolism in Tg mice was demonstrated. Also, with repeated scanning, the brain uptake values within a group and the hypometabolism of the Tg mice only remained stable over time when glucose correction was applied. Finally, hypometabolism was also observed in the cerebellum, yielding artificially higher rSUV values for Tg mice. Conclusion: Corrections for blood glucose levels have to be applied when semiquantifying 18 F-FDG brain uptake in mouse models for AD. Potential reference regions for normalization should be thoroughly investigated to ensure that they are not pathologically affected also by afferent connections.https://doi.org/10.1177/1536012115624919 |
| spellingShingle | Steven Deleye MSc Ann-Marie Waldron MSc Jill C. Richardson PhD Mark Schmidt PhD Xavier Langlois PhD Sigrid Stroobants MD, PhD Steven Staelens PhD The Effects of Physiological and Methodological Determinants on F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model Molecular Imaging |
| title | The Effects of Physiological and Methodological Determinants on F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model |
| title_full | The Effects of Physiological and Methodological Determinants on F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model |
| title_fullStr | The Effects of Physiological and Methodological Determinants on F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model |
| title_full_unstemmed | The Effects of Physiological and Methodological Determinants on F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model |
| title_short | The Effects of Physiological and Methodological Determinants on F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model |
| title_sort | effects of physiological and methodological determinants on f fdg mouse brain imaging exemplified in a double transgenic alzheimer model |
| url | https://doi.org/10.1177/1536012115624919 |
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