The Effect of a Total Fishmeal Replacement by <i>Arthrospira platensis</i> on the Microbiome of African Catfish (<i>Clarias gariepinus</i>)
An increasing number of fishmeal supplements are becoming the focus of aquaculture research, with a special emphasis on microalgae/cyanobacteria such as spirulina being considered as sustainable alternatives. New feed ingredients can have a far-reaching impact on the intestinal microbiome and theref...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2075-1729/11/6/558 |
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| author | Simon Rosenau Elisa Oertel Alexander Charles Mott Jens Tetens |
| author_facet | Simon Rosenau Elisa Oertel Alexander Charles Mott Jens Tetens |
| author_sort | Simon Rosenau |
| collection | DOAJ |
| description | An increasing number of fishmeal supplements are becoming the focus of aquaculture research, with a special emphasis on microalgae/cyanobacteria such as spirulina being considered as sustainable alternatives. New feed ingredients can have a far-reaching impact on the intestinal microbiome and therefore play an important role in the development and the health of fish. However, the influence of these alternatives on the microbiome is largely unknown. We undertook a 10 weeks feeding experiment on 120 African catfish with an initial body weight of 50.1 ± 2.95 g. To understand the effect of the spirulina supplementation, two isoenergetic experimental diets were formulated, containing either fishmeal or spirulina as a protein source. The 16S rRNA sequencing was used to analyze the intestinal bacteria microbiota. Results show that the observed richness indicated no significant statistical difference, but Chao1, ACE, Shannon, and Simpson indices indicate a possible increase in bacterial richness for the spirulina diet. The most abundant bacteria in both experimental groups were <i>Fusobacteriia</i> with the only taxa from the genus <i>Cetobacterium</i>. The bacterium from genus <i>Romboutsia</i> was more likely to be found in the microbiome of fish fed the fishmeal diet. In spirulina-fed fish, the genera <i>Plesiomonas</i> and <i>Bacteroides</i> were the most dominant microbes observed. Even though some genera were more abundant in the spirulina group, the overall microbial community structure was not affected by diets. |
| first_indexed | 2024-03-10T10:26:32Z |
| format | Article |
| id | doaj.art-a36bd84fbbe24719999dfb761f19212f |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T10:26:32Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-a36bd84fbbe24719999dfb761f19212f2023-11-22T00:01:04ZengMDPI AGLife2075-17292021-06-0111655810.3390/life11060558The Effect of a Total Fishmeal Replacement by <i>Arthrospira platensis</i> on the Microbiome of African Catfish (<i>Clarias gariepinus</i>)Simon Rosenau0Elisa Oertel1Alexander Charles Mott2Jens Tetens3Department of Animal Science, Georg-August-University of Göttingen, 37077 Göttingen, GermanyDepartment of Animal Science, Georg-August-University of Göttingen, 37077 Göttingen, GermanyDepartment of Animal Science, Georg-August-University of Göttingen, 37077 Göttingen, GermanyDepartment of Animal Science, Georg-August-University of Göttingen, 37077 Göttingen, GermanyAn increasing number of fishmeal supplements are becoming the focus of aquaculture research, with a special emphasis on microalgae/cyanobacteria such as spirulina being considered as sustainable alternatives. New feed ingredients can have a far-reaching impact on the intestinal microbiome and therefore play an important role in the development and the health of fish. However, the influence of these alternatives on the microbiome is largely unknown. We undertook a 10 weeks feeding experiment on 120 African catfish with an initial body weight of 50.1 ± 2.95 g. To understand the effect of the spirulina supplementation, two isoenergetic experimental diets were formulated, containing either fishmeal or spirulina as a protein source. The 16S rRNA sequencing was used to analyze the intestinal bacteria microbiota. Results show that the observed richness indicated no significant statistical difference, but Chao1, ACE, Shannon, and Simpson indices indicate a possible increase in bacterial richness for the spirulina diet. The most abundant bacteria in both experimental groups were <i>Fusobacteriia</i> with the only taxa from the genus <i>Cetobacterium</i>. The bacterium from genus <i>Romboutsia</i> was more likely to be found in the microbiome of fish fed the fishmeal diet. In spirulina-fed fish, the genera <i>Plesiomonas</i> and <i>Bacteroides</i> were the most dominant microbes observed. Even though some genera were more abundant in the spirulina group, the overall microbial community structure was not affected by diets.https://www.mdpi.com/2075-1729/11/6/558microbiomemicroalgaecyanobacteria<i>Arthrospira platensis</i><i>Clarias gariepinus</i>16S rRNA |
| spellingShingle | Simon Rosenau Elisa Oertel Alexander Charles Mott Jens Tetens The Effect of a Total Fishmeal Replacement by <i>Arthrospira platensis</i> on the Microbiome of African Catfish (<i>Clarias gariepinus</i>) Life microbiome microalgae cyanobacteria <i>Arthrospira platensis</i> <i>Clarias gariepinus</i> 16S rRNA |
| title | The Effect of a Total Fishmeal Replacement by <i>Arthrospira platensis</i> on the Microbiome of African Catfish (<i>Clarias gariepinus</i>) |
| title_full | The Effect of a Total Fishmeal Replacement by <i>Arthrospira platensis</i> on the Microbiome of African Catfish (<i>Clarias gariepinus</i>) |
| title_fullStr | The Effect of a Total Fishmeal Replacement by <i>Arthrospira platensis</i> on the Microbiome of African Catfish (<i>Clarias gariepinus</i>) |
| title_full_unstemmed | The Effect of a Total Fishmeal Replacement by <i>Arthrospira platensis</i> on the Microbiome of African Catfish (<i>Clarias gariepinus</i>) |
| title_short | The Effect of a Total Fishmeal Replacement by <i>Arthrospira platensis</i> on the Microbiome of African Catfish (<i>Clarias gariepinus</i>) |
| title_sort | effect of a total fishmeal replacement by i arthrospira platensis i on the microbiome of african catfish i clarias gariepinus i |
| topic | microbiome microalgae cyanobacteria <i>Arthrospira platensis</i> <i>Clarias gariepinus</i> 16S rRNA |
| url | https://www.mdpi.com/2075-1729/11/6/558 |
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