Enhancement of wound healing via topical application of natural products: In vitro and in vivo evaluations
Intact skin is the first physical barrier against all microbial infections. Thus, in the cases of wounds, burns, and skin damage, bacteria can infect and invade the deeper layers of skin to the bloodstream and other organs leading to severe illnesses. Thus, our study aims to investigate the potentia...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853522200185X |
| _version_ | 1818231475251707904 |
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| author | Abdallah S. Abdelsattar Salsabil Makky Rana Nofal Mariam Hebishy Mona M. Agwa Rania G. Aly Mohamed Y. Abo El-Naga Yehia A. Heikal Mohamed S. Fayez Nouran Rezk Ayman El-Shibiny |
| author_facet | Abdallah S. Abdelsattar Salsabil Makky Rana Nofal Mariam Hebishy Mona M. Agwa Rania G. Aly Mohamed Y. Abo El-Naga Yehia A. Heikal Mohamed S. Fayez Nouran Rezk Ayman El-Shibiny |
| author_sort | Abdallah S. Abdelsattar |
| collection | DOAJ |
| description | Intact skin is the first physical barrier against all microbial infections. Thus, in the cases of wounds, burns, and skin damage, bacteria can infect and invade the deeper layers of skin to the bloodstream and other organs leading to severe illnesses. Thus, our study aims to investigate the potential activity of natural products, propolis and honeybee venom, to control wound infections with multi-drug resistant Staphylococcus aureus (MDRSA) and safely accelerate the wound healing. First, this study characterized the clinically isolated S. aureus using biochemical, molecular, and antibiotic sensitivity tests. Then, the hydrogel was prepared via mixing chitosan with honey, propolis, and venom at different ratios, followed by physicochemical characterization and biological examination. The in vivo experiment results after topical application of optimum concentrations revealed that both venom and propolis have significant antibacterial activity at different temperatures. The IC50 of both propolis antioxidant and cytotoxicity assays was found to be 40.07 ± 2.18 μg/mL and 18.3 μg/mL, respectively. The cocktail bacteria showed both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 10 µg/mL and of 300 µg/mL with venom respectively & MIC and MBC of 100 µg/mL, 300 µg/mL with propolis respectively. The use of hydrogel was effective against wound infection and enhanced wound healing during 14 days. Before starting clinical trials, further studies can be done on large animal models. |
| first_indexed | 2024-12-12T10:50:59Z |
| format | Article |
| id | doaj.art-a36ed0477e75427d8c1b8f01f8e41378 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-12T10:50:59Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-a36ed0477e75427d8c1b8f01f8e413782022-12-22T00:26:47ZengElsevierArabian Journal of Chemistry1878-53522022-06-01156103869Enhancement of wound healing via topical application of natural products: In vitro and in vivo evaluationsAbdallah S. Abdelsattar0Salsabil Makky1Rana Nofal2Mariam Hebishy3Mona M. Agwa4Rania G. Aly5Mohamed Y. Abo El-Naga6Yehia A. Heikal7Mohamed S. Fayez8Nouran Rezk9Ayman El-Shibiny10Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza 12578, Egypt; Center for X-Ray and Determination of Structure of Matter, Zewail City of Science and Technology, October Gardens, 6th of October, Giza 12578, EgyptCenter for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza 12578, EgyptCenter for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza 12578, EgyptCenter for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza 12578, EgyptDepartment of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Giza 12622, EgyptDepartment of Surgical Pathology, Faculty of Medicine, Alexandria University, Alexandria, EgyptFood Science Department, Faculty of Agriculture, Ain Shams University, Cairo, EgyptFood Science Department, Faculty of Agriculture, Ain Shams University, Cairo, EgyptCenter for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza 12578, EgyptCenter for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza 12578, EgyptCenter for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza 12578, Egypt; Faculty of Environmental Agricultural Sciences, Arish University, Arish 45511, Egypt; Corresponding author at: Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza 12578, Egypt.Intact skin is the first physical barrier against all microbial infections. Thus, in the cases of wounds, burns, and skin damage, bacteria can infect and invade the deeper layers of skin to the bloodstream and other organs leading to severe illnesses. Thus, our study aims to investigate the potential activity of natural products, propolis and honeybee venom, to control wound infections with multi-drug resistant Staphylococcus aureus (MDRSA) and safely accelerate the wound healing. First, this study characterized the clinically isolated S. aureus using biochemical, molecular, and antibiotic sensitivity tests. Then, the hydrogel was prepared via mixing chitosan with honey, propolis, and venom at different ratios, followed by physicochemical characterization and biological examination. The in vivo experiment results after topical application of optimum concentrations revealed that both venom and propolis have significant antibacterial activity at different temperatures. The IC50 of both propolis antioxidant and cytotoxicity assays was found to be 40.07 ± 2.18 μg/mL and 18.3 μg/mL, respectively. The cocktail bacteria showed both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 10 µg/mL and of 300 µg/mL with venom respectively & MIC and MBC of 100 µg/mL, 300 µg/mL with propolis respectively. The use of hydrogel was effective against wound infection and enhanced wound healing during 14 days. Before starting clinical trials, further studies can be done on large animal models.http://www.sciencedirect.com/science/article/pii/S187853522200185XBee venomAntibacterialAntimicrobialAntibiotic-resistant bacteriaWound infectionPropolis |
| spellingShingle | Abdallah S. Abdelsattar Salsabil Makky Rana Nofal Mariam Hebishy Mona M. Agwa Rania G. Aly Mohamed Y. Abo El-Naga Yehia A. Heikal Mohamed S. Fayez Nouran Rezk Ayman El-Shibiny Enhancement of wound healing via topical application of natural products: In vitro and in vivo evaluations Arabian Journal of Chemistry Bee venom Antibacterial Antimicrobial Antibiotic-resistant bacteria Wound infection Propolis |
| title | Enhancement of wound healing via topical application of natural products: In vitro and in vivo evaluations |
| title_full | Enhancement of wound healing via topical application of natural products: In vitro and in vivo evaluations |
| title_fullStr | Enhancement of wound healing via topical application of natural products: In vitro and in vivo evaluations |
| title_full_unstemmed | Enhancement of wound healing via topical application of natural products: In vitro and in vivo evaluations |
| title_short | Enhancement of wound healing via topical application of natural products: In vitro and in vivo evaluations |
| title_sort | enhancement of wound healing via topical application of natural products in vitro and in vivo evaluations |
| topic | Bee venom Antibacterial Antimicrobial Antibiotic-resistant bacteria Wound infection Propolis |
| url | http://www.sciencedirect.com/science/article/pii/S187853522200185X |
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