Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion
Decoctions obtained from dried apple and pear fruits were subjected to in vitro digestion in the presence of a food matrix in order to determine changes in the polyphenol content and antiproliferative activity. The total phenolic and total proanthocyanidins contents were determined using spectrophot...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2018-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2018/0352-51391800073Z.pdf |
| _version_ | 1811277083126530048 |
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| author | Živković Jelena Šavikin Katarina Stanisavljević Nemanja Zdunić Gordana Stanojković Tatjana Samardžić Jelena |
| author_facet | Živković Jelena Šavikin Katarina Stanisavljević Nemanja Zdunić Gordana Stanojković Tatjana Samardžić Jelena |
| author_sort | Živković Jelena |
| collection | DOAJ |
| description | Decoctions obtained from dried apple and pear fruits were subjected to in vitro digestion in the presence of a food matrix in order to determine changes in the polyphenol content and antiproliferative activity. The total phenolic and total proanthocyanidins contents were determined using spectrophotometrical methods, and the phenolic compounds were analyzed by RP-HPLC/DAD before and after digestion. Chlorogenic acid and phlorizin dihydrate were the major identified compounds. The addition of a food matrix immediately decreased the contents of individual and total phenolics. After digestion, they were slightly elevated but still lower than in the initial samples. Antiproliferative activity was investigated on human epithelial carcinoma cell line (HeLa), human colon carcinoma (LS174) and human foetal lung fibroblast (MRC-5) cells. The exhibited growth inhibition was not correlated with the content of phenolics in the tested samples, indicating that it could not be explained solely by their content. With the exception of the apple decoction effect on HeLa cells, the digestion process resulted in significant increases in the antiproliferative activity. |
| first_indexed | 2024-04-13T00:10:27Z |
| format | Article |
| id | doaj.art-a36f4ed99bb04f1382abafe2ef5cb122 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-04-13T00:10:27Z |
| publishDate | 2018-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-a36f4ed99bb04f1382abafe2ef5cb1222022-12-22T03:11:07ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212018-01-0183121315132610.2298/JSC180604073Z0352-51391800073ZChemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestionŽivković Jelena0Šavikin Katarina1Stanisavljević Nemanja2Zdunić Gordana3Stanojković Tatjana4Samardžić Jelena5Institute for Medicinal Plants Research “Dr. Josif Pančić”, BelgradeInstitute for Medicinal Plants Research “Dr. Josif Pančić”, BelgradeInstitute of Molecular Genetics and Genetic Engineering, BelgradeInstitute for Medicinal Plants Research “Dr. Josif Pančić”, BelgradeInstitute of Oncology and Radiology of Serbia, BelgradeInstitute of Molecular Genetics and Genetic Engineering, BelgradeDecoctions obtained from dried apple and pear fruits were subjected to in vitro digestion in the presence of a food matrix in order to determine changes in the polyphenol content and antiproliferative activity. The total phenolic and total proanthocyanidins contents were determined using spectrophotometrical methods, and the phenolic compounds were analyzed by RP-HPLC/DAD before and after digestion. Chlorogenic acid and phlorizin dihydrate were the major identified compounds. The addition of a food matrix immediately decreased the contents of individual and total phenolics. After digestion, they were slightly elevated but still lower than in the initial samples. Antiproliferative activity was investigated on human epithelial carcinoma cell line (HeLa), human colon carcinoma (LS174) and human foetal lung fibroblast (MRC-5) cells. The exhibited growth inhibition was not correlated with the content of phenolics in the tested samples, indicating that it could not be explained solely by their content. With the exception of the apple decoction effect on HeLa cells, the digestion process resulted in significant increases in the antiproliferative activity.http://www.doiserbia.nb.rs/img/doi/0352-5139/2018/0352-51391800073Z.pdfbioaccessibilityphenolicsfood matrixfruit. |
| spellingShingle | Živković Jelena Šavikin Katarina Stanisavljević Nemanja Zdunić Gordana Stanojković Tatjana Samardžić Jelena Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion Journal of the Serbian Chemical Society bioaccessibility phenolics food matrix fruit. |
| title | Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion |
| title_full | Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion |
| title_fullStr | Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion |
| title_full_unstemmed | Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion |
| title_short | Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion |
| title_sort | chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion |
| topic | bioaccessibility phenolics food matrix fruit. |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2018/0352-51391800073Z.pdf |
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