The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumannii
Acinetobacter baumannii is an important, opportunistic nosocomial pathogen that causes a variety of nosocomial infections, and whose drug resistance rate has increased in recent years. The CRISPR-Cas system exists in several bacteria, providing adaptive immunity to foreign nucleic acid invasion. Thi...
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.797104/full |
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| author | Tingting Guo Tingting Guo Tingting Guo Xiaoli Sun Mengying Li Mengying Li Yuhang Wang Hongmei Jiao Guocai Li Guocai Li Guocai Li |
| author_facet | Tingting Guo Tingting Guo Tingting Guo Xiaoli Sun Mengying Li Mengying Li Yuhang Wang Hongmei Jiao Guocai Li Guocai Li Guocai Li |
| author_sort | Tingting Guo |
| collection | DOAJ |
| description | Acinetobacter baumannii is an important, opportunistic nosocomial pathogen that causes a variety of nosocomial infections, and whose drug resistance rate has increased in recent years. The CRISPR-Cas system exists in several bacteria, providing adaptive immunity to foreign nucleic acid invasion. This study explores whether CRISPR-Cas is related to drug resistance. Antibiotics were used to treat strains ATCC19606 and AB43, and the expression of CRISPR-related genes was found to be changed. The Csy proteins (Csy1–4) were previously detected to promote target recognition; however, the potential function of csy1 gene is still unknown. Thus, the RecAb homologous recombination system was utilized to knock out the csy1 gene from A. baumannii AB43, which carries the Type I-Fb CRISPR-Cas system, and to observe the drug resistance changes in wild-type and csy1-deleted strains. The AB43Δcsy1 mutant strain was found to become resistant to antibiotics, while the wild-type strain was sensitive to antibiotics. Moreover, transcriptome analysis revealed that the csy1 gene regulates genes encoding CRISPR-Cas-related proteins, drug-resistant efflux pumps, membrane proteins, and oxidative phosphorylation-related proteins, inhibiting antimicrobial resistance in A. baumannii. The in vitro resistance development assay revealed that the complete CRISPR-Cas system could inhibit the development of bacterial resistance. Our findings expand our understanding of the role of CRISPR-Cas csy1 gene in A. baumannii and link the CRISPR-Cas system to the biogenesis of bacterial drug-resistant structures. |
| first_indexed | 2024-04-11T18:16:35Z |
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| id | doaj.art-a374b9d183624a4b95ceaec074700dd6 |
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| issn | 2296-858X |
| language | English |
| last_indexed | 2024-04-11T18:16:35Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-a374b9d183624a4b95ceaec074700dd62022-12-22T04:09:53ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-01-01910.3389/fmed.2022.797104797104The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumanniiTingting Guo0Tingting Guo1Tingting Guo2Xiaoli Sun3Mengying Li4Mengying Li5Yuhang Wang6Hongmei Jiao7Guocai Li8Guocai Li9Guocai Li10Department of Microbiology, School of Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, ChinaDepartment of Microbiology, School of Medicine, Yangzhou University, Yangzhou, ChinaDepartment of Microbiology, School of Medicine, Yangzhou University, Yangzhou, ChinaDepartment of Pharmacy, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, ChinaDepartment of Microbiology, School of Medicine, Yangzhou University, Yangzhou, ChinaDepartment of Microbiology, School of Medicine, Yangzhou University, Yangzhou, ChinaDepartment of Microbiology, School of Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, ChinaAcinetobacter baumannii is an important, opportunistic nosocomial pathogen that causes a variety of nosocomial infections, and whose drug resistance rate has increased in recent years. The CRISPR-Cas system exists in several bacteria, providing adaptive immunity to foreign nucleic acid invasion. This study explores whether CRISPR-Cas is related to drug resistance. Antibiotics were used to treat strains ATCC19606 and AB43, and the expression of CRISPR-related genes was found to be changed. The Csy proteins (Csy1–4) were previously detected to promote target recognition; however, the potential function of csy1 gene is still unknown. Thus, the RecAb homologous recombination system was utilized to knock out the csy1 gene from A. baumannii AB43, which carries the Type I-Fb CRISPR-Cas system, and to observe the drug resistance changes in wild-type and csy1-deleted strains. The AB43Δcsy1 mutant strain was found to become resistant to antibiotics, while the wild-type strain was sensitive to antibiotics. Moreover, transcriptome analysis revealed that the csy1 gene regulates genes encoding CRISPR-Cas-related proteins, drug-resistant efflux pumps, membrane proteins, and oxidative phosphorylation-related proteins, inhibiting antimicrobial resistance in A. baumannii. The in vitro resistance development assay revealed that the complete CRISPR-Cas system could inhibit the development of bacterial resistance. Our findings expand our understanding of the role of CRISPR-Cas csy1 gene in A. baumannii and link the CRISPR-Cas system to the biogenesis of bacterial drug-resistant structures.https://www.frontiersin.org/articles/10.3389/fmed.2022.797104/fullAcinetobacter baumanniinosocomial pathogenCRISPR-Cas systemantimicrobial resistancecsy1 gene |
| spellingShingle | Tingting Guo Tingting Guo Tingting Guo Xiaoli Sun Mengying Li Mengying Li Yuhang Wang Hongmei Jiao Guocai Li Guocai Li Guocai Li The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumannii Frontiers in Medicine Acinetobacter baumannii nosocomial pathogen CRISPR-Cas system antimicrobial resistance csy1 gene |
| title | The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumannii |
| title_full | The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumannii |
| title_fullStr | The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumannii |
| title_full_unstemmed | The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumannii |
| title_short | The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumannii |
| title_sort | involvement of the csy1 gene in the antimicrobial resistance of acinetobacter baumannii |
| topic | Acinetobacter baumannii nosocomial pathogen CRISPR-Cas system antimicrobial resistance csy1 gene |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.797104/full |
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