Assessment of DNA damage in the heroin addicted population of Khyber Pakhtunkhwa, Pakistan using alkaline comet assay
The study was conducted to gain knowledge about the assessment of DNA damage in the population of heroin addicts and also to learn about the related health complications in drug abusers. A total of 103 samples of blood were collected from 65 heroin addicted subjects and 38 control subjects. For ev...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2022-04-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://rdo.psu.ac.th/sjst/journal/44-2/9.pdf |
| _version_ | 1798036863909363712 |
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| author | Muhammad Jawad Muhammad Zahid Faheem Ullah Mudassir Shah Abdul Wahab Muhammad Ismail Khan |
| author_facet | Muhammad Jawad Muhammad Zahid Faheem Ullah Mudassir Shah Abdul Wahab Muhammad Ismail Khan |
| author_sort | Muhammad Jawad |
| collection | DOAJ |
| description | The study was conducted to gain knowledge about the assessment of DNA damage in the population of heroin addicts
and also to learn about the related health complications in drug abusers. A total of 103 samples of blood were collected from 65
heroin addicted subjects and 38 control subjects. For evaluation of DNA damage in the heroin addicted group, a comet assay
technique was carried out. The results demonstrated that significant DNA damage 167.35±16.49 (P<0.05) was found in subjects
who were addicted to heroin as compared to the control group having cells without any DNA damage 53.02±25.03. The
population which were positive for human immunodeficiency virus infection had the highest total comet score values followed
by asthma and hepatitis C virus. The total comet score increased significantly with age group. Significantly, the highest
genotoxicity was concluded in the heroin addicted population. |
| first_indexed | 2024-04-11T21:18:49Z |
| format | Article |
| id | doaj.art-a376f6bdbcbb451c9aa2c49a0cbfaca6 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-11T21:18:49Z |
| publishDate | 2022-04-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-a376f6bdbcbb451c9aa2c49a0cbfaca62022-12-22T04:02:43ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952022-04-0144234835210.14456/sjst-psu.2022.49Assessment of DNA damage in the heroin addicted population of Khyber Pakhtunkhwa, Pakistan using alkaline comet assayMuhammad Jawad0Muhammad Zahid1Faheem Ullah2Mudassir Shah3Abdul Wahab4Muhammad Ismail Khan5Department of Zoology, Isalmia College Peshawar, Khyber Pakhtunkhwa, PakistanDepartment of Zoology, Isalmia College Peshawar, Khyber Pakhtunkhwa, PakistanDepartment of Pediatrics Medicine, Type C Hospital, Karak, Khyber Pakhtunkhwa, PakistanDepartment of Zoology, Government College Peshawar, Khyber Pakhtunkhwa, PakistanDepartment of Zoology, Isalmia College Peshawar, Khyber Pakhtunkhwa, PakistanDepartment of Zoology, Isalmia College Peshawar, Khyber Pakhtunkhwa, PakistanThe study was conducted to gain knowledge about the assessment of DNA damage in the population of heroin addicts and also to learn about the related health complications in drug abusers. A total of 103 samples of blood were collected from 65 heroin addicted subjects and 38 control subjects. For evaluation of DNA damage in the heroin addicted group, a comet assay technique was carried out. The results demonstrated that significant DNA damage 167.35±16.49 (P<0.05) was found in subjects who were addicted to heroin as compared to the control group having cells without any DNA damage 53.02±25.03. The population which were positive for human immunodeficiency virus infection had the highest total comet score values followed by asthma and hepatitis C virus. The total comet score increased significantly with age group. Significantly, the highest genotoxicity was concluded in the heroin addicted population.https://rdo.psu.ac.th/sjst/journal/44-2/9.pdfheroin addicted populationdna damagecomet assaygenotoxicity |
| spellingShingle | Muhammad Jawad Muhammad Zahid Faheem Ullah Mudassir Shah Abdul Wahab Muhammad Ismail Khan Assessment of DNA damage in the heroin addicted population of Khyber Pakhtunkhwa, Pakistan using alkaline comet assay Songklanakarin Journal of Science and Technology (SJST) heroin addicted population dna damage comet assay genotoxicity |
| title | Assessment of DNA damage in the heroin addicted population of Khyber Pakhtunkhwa, Pakistan using alkaline comet assay |
| title_full | Assessment of DNA damage in the heroin addicted population of Khyber Pakhtunkhwa, Pakistan using alkaline comet assay |
| title_fullStr | Assessment of DNA damage in the heroin addicted population of Khyber Pakhtunkhwa, Pakistan using alkaline comet assay |
| title_full_unstemmed | Assessment of DNA damage in the heroin addicted population of Khyber Pakhtunkhwa, Pakistan using alkaline comet assay |
| title_short | Assessment of DNA damage in the heroin addicted population of Khyber Pakhtunkhwa, Pakistan using alkaline comet assay |
| title_sort | assessment of dna damage in the heroin addicted population of khyber pakhtunkhwa pakistan using alkaline comet assay |
| topic | heroin addicted population dna damage comet assay genotoxicity |
| url | https://rdo.psu.ac.th/sjst/journal/44-2/9.pdf |
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