Development of a pseudo-typed virus particle based method to determine the efficacy of virucidal agents
Abstract The ongoing Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has highlighted the threat that viral outbreaks pose to global health. A key tool in the arsenal to prevent and control viral disease outbreaks is disinfection of equipment and surfaces with formulations that...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52177-2 |
| _version_ | 1797276627289243648 |
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| author | Jordan Thomas Farah Mughal Kelly J. Roper Aurelia Kotsiri Wejdan Albalawi Abdullateef Alshehri Yugandhar B. S. Reddy Sayandip Mukherjee Georgios Pollakis William A. Paxton Michael Hoptroff |
| author_facet | Jordan Thomas Farah Mughal Kelly J. Roper Aurelia Kotsiri Wejdan Albalawi Abdullateef Alshehri Yugandhar B. S. Reddy Sayandip Mukherjee Georgios Pollakis William A. Paxton Michael Hoptroff |
| author_sort | Jordan Thomas |
| collection | DOAJ |
| description | Abstract The ongoing Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has highlighted the threat that viral outbreaks pose to global health. A key tool in the arsenal to prevent and control viral disease outbreaks is disinfection of equipment and surfaces with formulations that contain virucidal agents (VA). However, assessment of the efficacy of virus inactivation often requires live virus assays or surrogate viruses such as Modified Vaccinia Virus Ankara (MVA), which can be expensive, time consuming and technically challenging. Therefore, we have developed a pseudo-typed virus (PV) based approach to assess the inactivation of enveloped viruses with a fast and quantitative output that can be adapted to emerging viruses. Additionally, we have developed a method to completely remove the cytotoxicity of virucidal agents while retaining the required sensitivity to measure PV infectivity. Our results indicated that the removal of cytotoxicity was an essential step to accurately measure virus inactivation. Further, we demonstrated that there was no difference in susceptibility to virus inactivation between PVs that express the envelopes of HIV-1, SARS-CoV-2, and Influenza A/Indonesia. Therefore, we have developed an effective and safe alternative to live virus assays that enables the rapid assessment of virucidal activity for the development and optimization of virucidal reagents. |
| first_indexed | 2024-03-07T15:30:57Z |
| format | Article |
| id | doaj.art-a37989ac726241ffbe3d975b667de77c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:30:57Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a37989ac726241ffbe3d975b667de77c2024-03-05T16:25:53ZengNature PortfolioScientific Reports2045-23222024-01-0114111210.1038/s41598-024-52177-2Development of a pseudo-typed virus particle based method to determine the efficacy of virucidal agentsJordan Thomas0Farah Mughal1Kelly J. Roper2Aurelia Kotsiri3Wejdan Albalawi4Abdullateef Alshehri5Yugandhar B. S. Reddy6Sayandip Mukherjee7Georgios Pollakis8William A. Paxton9Michael Hoptroff10Department of Clinical Infection, Microbiology and Immunology (CIMI), Institute of Infection, Veterinary and Ecological Sciences (IVES), University of LiverpoolDepartment of Clinical Infection, Microbiology and Immunology (CIMI), Institute of Infection, Veterinary and Ecological Sciences (IVES), University of LiverpoolDepartment of Clinical Infection, Microbiology and Immunology (CIMI), Institute of Infection, Veterinary and Ecological Sciences (IVES), University of LiverpoolDepartment of Clinical Infection, Microbiology and Immunology (CIMI), Institute of Infection, Veterinary and Ecological Sciences (IVES), University of LiverpoolDepartment of Clinical Infection, Microbiology and Immunology (CIMI), Institute of Infection, Veterinary and Ecological Sciences (IVES), University of LiverpoolDepartment of Clinical Infection, Microbiology and Immunology (CIMI), Institute of Infection, Veterinary and Ecological Sciences (IVES), University of LiverpoolUnilever Research & Development CentreUnilever Research & Development CentreDepartment of Clinical Infection, Microbiology and Immunology (CIMI), Institute of Infection, Veterinary and Ecological Sciences (IVES), University of LiverpoolDepartment of Clinical Infection, Microbiology and Immunology (CIMI), Institute of Infection, Veterinary and Ecological Sciences (IVES), University of LiverpoolUnilever Research & DevelopmentAbstract The ongoing Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has highlighted the threat that viral outbreaks pose to global health. A key tool in the arsenal to prevent and control viral disease outbreaks is disinfection of equipment and surfaces with formulations that contain virucidal agents (VA). However, assessment of the efficacy of virus inactivation often requires live virus assays or surrogate viruses such as Modified Vaccinia Virus Ankara (MVA), which can be expensive, time consuming and technically challenging. Therefore, we have developed a pseudo-typed virus (PV) based approach to assess the inactivation of enveloped viruses with a fast and quantitative output that can be adapted to emerging viruses. Additionally, we have developed a method to completely remove the cytotoxicity of virucidal agents while retaining the required sensitivity to measure PV infectivity. Our results indicated that the removal of cytotoxicity was an essential step to accurately measure virus inactivation. Further, we demonstrated that there was no difference in susceptibility to virus inactivation between PVs that express the envelopes of HIV-1, SARS-CoV-2, and Influenza A/Indonesia. Therefore, we have developed an effective and safe alternative to live virus assays that enables the rapid assessment of virucidal activity for the development and optimization of virucidal reagents.https://doi.org/10.1038/s41598-024-52177-2 |
| spellingShingle | Jordan Thomas Farah Mughal Kelly J. Roper Aurelia Kotsiri Wejdan Albalawi Abdullateef Alshehri Yugandhar B. S. Reddy Sayandip Mukherjee Georgios Pollakis William A. Paxton Michael Hoptroff Development of a pseudo-typed virus particle based method to determine the efficacy of virucidal agents Scientific Reports |
| title | Development of a pseudo-typed virus particle based method to determine the efficacy of virucidal agents |
| title_full | Development of a pseudo-typed virus particle based method to determine the efficacy of virucidal agents |
| title_fullStr | Development of a pseudo-typed virus particle based method to determine the efficacy of virucidal agents |
| title_full_unstemmed | Development of a pseudo-typed virus particle based method to determine the efficacy of virucidal agents |
| title_short | Development of a pseudo-typed virus particle based method to determine the efficacy of virucidal agents |
| title_sort | development of a pseudo typed virus particle based method to determine the efficacy of virucidal agents |
| url | https://doi.org/10.1038/s41598-024-52177-2 |
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