Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery
The mouse model of multiple cerebral infarctions, established by injecting fluorescent microspheres into the common carotid artery, is a recent development in animal models of cerebral ischemia. To investigate its effectiveness, mouse models of cerebral infarction were created by injecting fluoresce...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=4;spage=832;epage=837;aulast=Shen |
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| author | Yi Shen Ming-Jiang Yao Yu-Xin Su Dong-Sheng Xu Jia Wang Guang-Rui Wang Jing-Jing Cui Jian-Liang Zhang Wan-Zhu Bai |
| author_facet | Yi Shen Ming-Jiang Yao Yu-Xin Su Dong-Sheng Xu Jia Wang Guang-Rui Wang Jing-Jing Cui Jian-Liang Zhang Wan-Zhu Bai |
| author_sort | Yi Shen |
| collection | DOAJ |
| description | The mouse model of multiple cerebral infarctions, established by injecting fluorescent microspheres into the common carotid artery, is a recent development in animal models of cerebral ischemia. To investigate its effectiveness, mouse models of cerebral infarction were created by injecting fluorescent microspheres, 45–53 µm in diameter, into the common carotid artery. Six hours after modeling, fluorescent microspheres were observed directly through a fluorescence stereomicroscope, both on the brain surface and in brain sections. Changes in blood vessels, neurons and glial cells associated with microinfarcts were examined using fluorescence histochemistry and immunohistochemistry. The microspheres were distributed mainly in the cerebral cortex, striatum and hippocampus ipsilateral to the side of injection. Microinfarcts were found in the brain regions where the fluorescent microspheres were present. Here the lodged microspheres induced vascular and neuronal injury and the activation of astroglia and microglia. These histopathological changes indicate that this animal model of multiple cerebral infarctions effectively simulates the changes of various cell types observed in multifocal microinfarcts. This model is an effective, additional tool to study the pathogenesis of ischemic stroke and could be used to evaluate therapeutic interventions. This study was approved by the Animal Ethics Committee of the Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences (approval No. D2021-03-16-1) on March 16, 2021. |
| first_indexed | 2024-12-18T01:13:54Z |
| format | Article |
| id | doaj.art-a37dac9dbff2461dbcb73ac241468203 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-18T01:13:54Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-a37dac9dbff2461dbcb73ac2414682032022-12-21T21:26:01ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-0117483283710.4103/1673-5374.322470Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid arteryYi ShenMing-Jiang YaoYu-Xin SuDong-Sheng XuJia WangGuang-Rui WangJing-Jing CuiJian-Liang ZhangWan-Zhu BaiThe mouse model of multiple cerebral infarctions, established by injecting fluorescent microspheres into the common carotid artery, is a recent development in animal models of cerebral ischemia. To investigate its effectiveness, mouse models of cerebral infarction were created by injecting fluorescent microspheres, 45–53 µm in diameter, into the common carotid artery. Six hours after modeling, fluorescent microspheres were observed directly through a fluorescence stereomicroscope, both on the brain surface and in brain sections. Changes in blood vessels, neurons and glial cells associated with microinfarcts were examined using fluorescence histochemistry and immunohistochemistry. The microspheres were distributed mainly in the cerebral cortex, striatum and hippocampus ipsilateral to the side of injection. Microinfarcts were found in the brain regions where the fluorescent microspheres were present. Here the lodged microspheres induced vascular and neuronal injury and the activation of astroglia and microglia. These histopathological changes indicate that this animal model of multiple cerebral infarctions effectively simulates the changes of various cell types observed in multifocal microinfarcts. This model is an effective, additional tool to study the pathogenesis of ischemic stroke and could be used to evaluate therapeutic interventions. This study was approved by the Animal Ethics Committee of the Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences (approval No. D2021-03-16-1) on March 16, 2021.http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=4;spage=832;epage=837;aulast=Shenastrocytes; blood-brain barrier; common carotid artery; fluorescent microsphere; histochemistry; ischemia; microglia; microinfarcts; neuron; neurovascular unit; stroke |
| spellingShingle | Yi Shen Ming-Jiang Yao Yu-Xin Su Dong-Sheng Xu Jia Wang Guang-Rui Wang Jing-Jing Cui Jian-Liang Zhang Wan-Zhu Bai Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery Neural Regeneration Research astrocytes; blood-brain barrier; common carotid artery; fluorescent microsphere; histochemistry; ischemia; microglia; microinfarcts; neuron; neurovascular unit; stroke |
| title | Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery |
| title_full | Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery |
| title_fullStr | Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery |
| title_full_unstemmed | Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery |
| title_short | Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery |
| title_sort | histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery |
| topic | astrocytes; blood-brain barrier; common carotid artery; fluorescent microsphere; histochemistry; ischemia; microglia; microinfarcts; neuron; neurovascular unit; stroke |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=4;spage=832;epage=837;aulast=Shen |
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