Frequency of two VKORC1 gene variants and its correlation with warfarin maintenance dose
Warfarin is a commonly-prescribed anticoagulant used to treat and prevent thromboembolic events. The requirement for varying doses of warfarin depends on genetic and environmental components. In this study, the frequency of two single-nucleotide polymorphic variants of the vitamin K epoxide reductas...
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Kharazmi University
2018-12-01
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| Series: | یافتههای نوین در علوم زیستی |
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| Online Access: | http://nbr.khu.ac.ir/browse.php?a_code=A-10-783-1&slc_lang=en&sid=1 |
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| author | esmat khaleqsefat Mohammad Khalaj-Kondori Morteza Jabbarpour bonyadi Hamid Soraya Behnam Askari |
| author_facet | esmat khaleqsefat Mohammad Khalaj-Kondori Morteza Jabbarpour bonyadi Hamid Soraya Behnam Askari |
| author_sort | esmat khaleqsefat |
| collection | DOAJ |
| description | Warfarin is a commonly-prescribed anticoagulant used to treat and prevent thromboembolic events. The requirement for varying doses of warfarin depends on genetic and environmental components. In this study, the frequency of two single-nucleotide polymorphic variants of the vitamin K epoxide reductase complex subunit 1 (VKORC1) gene (1173 C>T (rs9934438) and 3730 G>A (rs7294)) and its correlation with warfarin maintenance doses were investigated in patients with heart valve replacement from West Azarbayejan, Iran. Blood samples were obtained from 185 patients; their genomic DNA was extracted and samples were genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) assay. To assess if the blood warfarin level is different among genotypes, we used a one-way analysis of variance (ANOVA) followed by a Tukey’s post-hoc comparison. The minor allele frequency was determined to be 54% for 1173T and 53.7% for 3730A. Patients who carried the G allele at position 3730 and T allele at position 1173 required a significantly lower daily mean warfarin dosage (P <0.001). Consideration of the VKORC1 gene polymorphism, especially at the initial stages of the therapy, can be helpful in pre-treatment dosing of warfarin, which, in turn, reduces the adverse effects resulting from inappropriate drug prescription. |
| first_indexed | 2024-12-14T00:59:35Z |
| format | Article |
| id | doaj.art-a37e695ae98f44d9b7f7b8b0ef03fa4a |
| institution | Directory Open Access Journal |
| issn | 2423-6330 2476-7115 |
| language | English |
| last_indexed | 2024-12-14T00:59:35Z |
| publishDate | 2018-12-01 |
| publisher | Kharazmi University |
| record_format | Article |
| series | یافتههای نوین در علوم زیستی |
| spelling | doaj.art-a37e695ae98f44d9b7f7b8b0ef03fa4a2022-12-21T23:23:20ZengKharazmi Universityیافتههای نوین در علوم زیستی2423-63302476-71152018-12-0153237243Frequency of two VKORC1 gene variants and its correlation with warfarin maintenance doseesmat khaleqsefat0Mohammad Khalaj-Kondori1Morteza Jabbarpour bonyadi2Hamid Soraya3Behnam Askari4 Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Department of Pharmacology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran Department of Cardiovascular Surgery, Seyyed-al-Shohada Heart Center, Urmia University of Medical Sciences, Urmia, Iran Warfarin is a commonly-prescribed anticoagulant used to treat and prevent thromboembolic events. The requirement for varying doses of warfarin depends on genetic and environmental components. In this study, the frequency of two single-nucleotide polymorphic variants of the vitamin K epoxide reductase complex subunit 1 (VKORC1) gene (1173 C>T (rs9934438) and 3730 G>A (rs7294)) and its correlation with warfarin maintenance doses were investigated in patients with heart valve replacement from West Azarbayejan, Iran. Blood samples were obtained from 185 patients; their genomic DNA was extracted and samples were genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) assay. To assess if the blood warfarin level is different among genotypes, we used a one-way analysis of variance (ANOVA) followed by a Tukey’s post-hoc comparison. The minor allele frequency was determined to be 54% for 1173T and 53.7% for 3730A. Patients who carried the G allele at position 3730 and T allele at position 1173 required a significantly lower daily mean warfarin dosage (P <0.001). Consideration of the VKORC1 gene polymorphism, especially at the initial stages of the therapy, can be helpful in pre-treatment dosing of warfarin, which, in turn, reduces the adverse effects resulting from inappropriate drug prescription.http://nbr.khu.ac.ir/browse.php?a_code=A-10-783-1&slc_lang=en&sid=1drug dosepersonalised treatmentpolymerase chain reactionrestriction fragment length polymorphism |
| spellingShingle | esmat khaleqsefat Mohammad Khalaj-Kondori Morteza Jabbarpour bonyadi Hamid Soraya Behnam Askari Frequency of two VKORC1 gene variants and its correlation with warfarin maintenance dose یافتههای نوین در علوم زیستی drug dose personalised treatment polymerase chain reaction restriction fragment length polymorphism |
| title | Frequency of two VKORC1 gene variants and its correlation with warfarin maintenance dose |
| title_full | Frequency of two VKORC1 gene variants and its correlation with warfarin maintenance dose |
| title_fullStr | Frequency of two VKORC1 gene variants and its correlation with warfarin maintenance dose |
| title_full_unstemmed | Frequency of two VKORC1 gene variants and its correlation with warfarin maintenance dose |
| title_short | Frequency of two VKORC1 gene variants and its correlation with warfarin maintenance dose |
| title_sort | frequency of two vkorc1 gene variants and its correlation with warfarin maintenance dose |
| topic | drug dose personalised treatment polymerase chain reaction restriction fragment length polymorphism |
| url | http://nbr.khu.ac.ir/browse.php?a_code=A-10-783-1&slc_lang=en&sid=1 |
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