Purification of Crude Fructo-Oligosaccharide Preparations Using Probiotic Bacteria for the Selective Fermentation of Monosaccharide Byproducts
Probiotics are microbes that promote health when consumed in sufficient amounts. They are present in many fermented foods or can be provided directly as supplements. Probiotics utilize non-digestible prebiotic oligosaccharides for growth in the intestinal tract, contributing to a healthy microbiome....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.620626/full |
| _version_ | 1818716216328454144 |
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| author | Rong Fan Rong Fan Jan Philipp Burghardt Jan Philipp Burghardt Jinqing Huang Tao Xiong Peter Czermak Peter Czermak Peter Czermak |
| author_facet | Rong Fan Rong Fan Jan Philipp Burghardt Jan Philipp Burghardt Jinqing Huang Tao Xiong Peter Czermak Peter Czermak Peter Czermak |
| author_sort | Rong Fan |
| collection | DOAJ |
| description | Probiotics are microbes that promote health when consumed in sufficient amounts. They are present in many fermented foods or can be provided directly as supplements. Probiotics utilize non-digestible prebiotic oligosaccharides for growth in the intestinal tract, contributing to a healthy microbiome. The oligosaccharides favored by probiotics are species-dependent, as shown by the selective utilization of substrates in mixed sugar solutions such as crude fructo-oligosaccharides (FOS). Enzymatically produced crude FOS preparations contain abundant monosaccharide byproducts, residual sucrose, and FOS varying in chain length. Here we investigated the metabolic profiles of four probiotic bacteria during the batch fermentation of crude FOS under controlled conditions. We found that Bacillus subtilis rapidly utilized most of the monosaccharides but little sucrose or FOS. We therefore tested the feasibility of a microbial fed-batch fermentation process for the purification of FOS from crude preparations, which increased the purity of FOS from 59.2 to 82.5% with a final concentration of 140 g·l−1. We also tested cell immobilization in alginate beads as a means to remove monosaccharides from crude FOS. This encapsulation concept establishes the basis for new synbiotic formulations that combine probiotic microbes and prebiotic oligosaccharides. |
| first_indexed | 2024-12-17T19:15:44Z |
| format | Article |
| id | doaj.art-a382c3a2ce5d42c3a39e59ed58c787b6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T19:15:44Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a382c3a2ce5d42c3a39e59ed58c787b62022-12-21T21:35:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-01-011110.3389/fmicb.2020.620626620626Purification of Crude Fructo-Oligosaccharide Preparations Using Probiotic Bacteria for the Selective Fermentation of Monosaccharide ByproductsRong Fan0Rong Fan1Jan Philipp Burghardt2Jan Philipp Burghardt3Jinqing Huang4Tao Xiong5Peter Czermak6Peter Czermak7Peter Czermak8Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen, GermanyDepartment of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Giessen, GermanyInstitute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen, GermanyFaculty of Biology and Chemistry, Justus Liebig University, Giessen, GermanyState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, ChinaInstitute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen, GermanyDepartment of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Giessen, GermanyFaculty of Biology and Chemistry, Justus Liebig University, Giessen, GermanyProbiotics are microbes that promote health when consumed in sufficient amounts. They are present in many fermented foods or can be provided directly as supplements. Probiotics utilize non-digestible prebiotic oligosaccharides for growth in the intestinal tract, contributing to a healthy microbiome. The oligosaccharides favored by probiotics are species-dependent, as shown by the selective utilization of substrates in mixed sugar solutions such as crude fructo-oligosaccharides (FOS). Enzymatically produced crude FOS preparations contain abundant monosaccharide byproducts, residual sucrose, and FOS varying in chain length. Here we investigated the metabolic profiles of four probiotic bacteria during the batch fermentation of crude FOS under controlled conditions. We found that Bacillus subtilis rapidly utilized most of the monosaccharides but little sucrose or FOS. We therefore tested the feasibility of a microbial fed-batch fermentation process for the purification of FOS from crude preparations, which increased the purity of FOS from 59.2 to 82.5% with a final concentration of 140 g·l−1. We also tested cell immobilization in alginate beads as a means to remove monosaccharides from crude FOS. This encapsulation concept establishes the basis for new synbiotic formulations that combine probiotic microbes and prebiotic oligosaccharides.https://www.frontiersin.org/articles/10.3389/fmicb.2020.620626/fullprobiotic microorganismprebioticssugar metabolismfructo-oligosaccharides purificationsynbiotics |
| spellingShingle | Rong Fan Rong Fan Jan Philipp Burghardt Jan Philipp Burghardt Jinqing Huang Tao Xiong Peter Czermak Peter Czermak Peter Czermak Purification of Crude Fructo-Oligosaccharide Preparations Using Probiotic Bacteria for the Selective Fermentation of Monosaccharide Byproducts Frontiers in Microbiology probiotic microorganism prebiotics sugar metabolism fructo-oligosaccharides purification synbiotics |
| title | Purification of Crude Fructo-Oligosaccharide Preparations Using Probiotic Bacteria for the Selective Fermentation of Monosaccharide Byproducts |
| title_full | Purification of Crude Fructo-Oligosaccharide Preparations Using Probiotic Bacteria for the Selective Fermentation of Monosaccharide Byproducts |
| title_fullStr | Purification of Crude Fructo-Oligosaccharide Preparations Using Probiotic Bacteria for the Selective Fermentation of Monosaccharide Byproducts |
| title_full_unstemmed | Purification of Crude Fructo-Oligosaccharide Preparations Using Probiotic Bacteria for the Selective Fermentation of Monosaccharide Byproducts |
| title_short | Purification of Crude Fructo-Oligosaccharide Preparations Using Probiotic Bacteria for the Selective Fermentation of Monosaccharide Byproducts |
| title_sort | purification of crude fructo oligosaccharide preparations using probiotic bacteria for the selective fermentation of monosaccharide byproducts |
| topic | probiotic microorganism prebiotics sugar metabolism fructo-oligosaccharides purification synbiotics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.620626/full |
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