Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair
Mechanically processed stromal vascular fraction (mSVF) is a highly interesting cell source for regenerative purposes, including wound healing, and a practical alternative to enzymatically isolated SVF. In the clinical context, SVF benefits from scaffolds that facilitate viability and other cellular...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13944 |
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| author | Mauro Vasella Kevin Arnke Dalia Dranseikiene Elia Guzzi Francesca Melega Gregory Reid Holger Jan Klein Riccardo Schweizer Mark W. Tibbitt Bong-Sung Kim |
| author_facet | Mauro Vasella Kevin Arnke Dalia Dranseikiene Elia Guzzi Francesca Melega Gregory Reid Holger Jan Klein Riccardo Schweizer Mark W. Tibbitt Bong-Sung Kim |
| author_sort | Mauro Vasella |
| collection | DOAJ |
| description | Mechanically processed stromal vascular fraction (mSVF) is a highly interesting cell source for regenerative purposes, including wound healing, and a practical alternative to enzymatically isolated SVF. In the clinical context, SVF benefits from scaffolds that facilitate viability and other cellular properties. In the present work, the feasibility of methacrylated gelatin (GelMA), a stiffness-tunable, light-inducible hydrogel with high biocompatibility is investigated as a scaffold for SVF in an in vitro setting. Lipoaspirates from elective surgical procedures were collected and processed to mSVF and mixed with GelMA precursor solutions. Non-encapsulated mSVF served as a control. Viability was measured over 21 days. Secreted basic fibroblast growth factor (bFGF) levels were measured on days 1, 7 and 21 by ELISA. IHC was performed to detect VEGF-A, perilipin-2, and CD73 expression on days 7 and 21. The impact of GelMA-mSVF on human dermal fibroblasts was measured in a co-culture assay by the same viability assay. The viability of cultured GelMA-mSVF was significantly higher after 21 days (<i>p</i> < 0.01) when compared to mSVF alone. Also, GelMA-mSVF secreted stable levels of bFGF over 21 days. While VEGF-A was primarily expressed on day 21, perilipin-2 and CD73-positive cells were observed on days 7 and 21. Finally, GelMA-mSVF significantly improved fibroblast viability as compared with GelMA alone (<i>p</i> < 0.01). GelMA may be a promising scaffold for mSVF as it maintains cell viability and proliferation with the release of growth factors while facilitating adipogenic differentiation, stromal cell marker expression and fibroblast proliferation. |
| first_indexed | 2024-03-10T22:40:31Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:31Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a383c5edc8ea4fbeaad7523588ca12482023-11-19T11:05:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181394410.3390/ijms241813944Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound RepairMauro Vasella0Kevin Arnke1Dalia Dranseikiene2Elia Guzzi3Francesca Melega4Gregory Reid5Holger Jan Klein6Riccardo Schweizer7Mark W. Tibbitt8Bong-Sung Kim9Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, 8091 Zurich, SwitzerlandCenter for Preclinical Development, University Hospital Zurich, 8091 Zurich, SwitzerlandMacromolecular Engineering Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich, SwitzerlandMacromolecular Engineering Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich, SwitzerlandInstitute of Pathology and Molecular Pathology, University Hospital Zurich, 8091 Zurich, SwitzerlandDepartment of Plastic Surgery and Hand Surgery, University Hospital Zurich, 8091 Zurich, SwitzerlandDepartment of Plastic Surgery and Hand Surgery, Cantonal Hospital Aarau, 5001 Aarau, SwitzerlandDepartment of Plastic, Reconstructive and Aesthetic Surgery, Regional Hospital Lugano, 6900 Lugano, SwitzerlandMacromolecular Engineering Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich, SwitzerlandDepartment of Plastic Surgery and Hand Surgery, University Hospital Zurich, 8091 Zurich, SwitzerlandMechanically processed stromal vascular fraction (mSVF) is a highly interesting cell source for regenerative purposes, including wound healing, and a practical alternative to enzymatically isolated SVF. In the clinical context, SVF benefits from scaffolds that facilitate viability and other cellular properties. In the present work, the feasibility of methacrylated gelatin (GelMA), a stiffness-tunable, light-inducible hydrogel with high biocompatibility is investigated as a scaffold for SVF in an in vitro setting. Lipoaspirates from elective surgical procedures were collected and processed to mSVF and mixed with GelMA precursor solutions. Non-encapsulated mSVF served as a control. Viability was measured over 21 days. Secreted basic fibroblast growth factor (bFGF) levels were measured on days 1, 7 and 21 by ELISA. IHC was performed to detect VEGF-A, perilipin-2, and CD73 expression on days 7 and 21. The impact of GelMA-mSVF on human dermal fibroblasts was measured in a co-culture assay by the same viability assay. The viability of cultured GelMA-mSVF was significantly higher after 21 days (<i>p</i> < 0.01) when compared to mSVF alone. Also, GelMA-mSVF secreted stable levels of bFGF over 21 days. While VEGF-A was primarily expressed on day 21, perilipin-2 and CD73-positive cells were observed on days 7 and 21. Finally, GelMA-mSVF significantly improved fibroblast viability as compared with GelMA alone (<i>p</i> < 0.01). GelMA may be a promising scaffold for mSVF as it maintains cell viability and proliferation with the release of growth factors while facilitating adipogenic differentiation, stromal cell marker expression and fibroblast proliferation.https://www.mdpi.com/1422-0067/24/18/13944hydrogelGelMAnatural compoundadipose-derived stromal cellsSVFregenerative medicine |
| spellingShingle | Mauro Vasella Kevin Arnke Dalia Dranseikiene Elia Guzzi Francesca Melega Gregory Reid Holger Jan Klein Riccardo Schweizer Mark W. Tibbitt Bong-Sung Kim Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair International Journal of Molecular Sciences hydrogel GelMA natural compound adipose-derived stromal cells SVF regenerative medicine |
| title | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
| title_full | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
| title_fullStr | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
| title_full_unstemmed | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
| title_short | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
| title_sort | methacrylated gelatin as a scaffold for mechanically isolated stromal vascular fraction for cutaneous wound repair |
| topic | hydrogel GelMA natural compound adipose-derived stromal cells SVF regenerative medicine |
| url | https://www.mdpi.com/1422-0067/24/18/13944 |
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