Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different Reagents
Methoxy poly(ethylene glycol) (mPEG) with molecular mass of 5 kDa activated with succinimidyl carbonate and cyanuric chloride, separately was covalently attached to human red blood cells (RBCs). Inhibition of agglutination by blood-type specific antisera (anti-D) was employed to evaluate the effect...
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| Format: | Article |
| Language: | English |
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2008-09-01
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| Series: | Iranian Journal of Chemistry & Chemical Engineering |
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| Online Access: | http://www.ijcce.ac.ir/article_6923_195a6340a2016640d1810451c86a8c01.pdf |
| _version_ | 1811283954030870528 |
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| author | Fatemeh Sarvi Sameereh Hashemi Najafabadi Ebrahim Vasheghani Farahani Seyed Abbas Shojaosadati |
| author_facet | Fatemeh Sarvi Sameereh Hashemi Najafabadi Ebrahim Vasheghani Farahani Seyed Abbas Shojaosadati |
| author_sort | Fatemeh Sarvi |
| collection | DOAJ |
| description | Methoxy poly(ethylene glycol) (mPEG) with molecular mass of 5 kDa activated with succinimidyl carbonate and cyanuric chloride, separately was covalently attached to human red blood cells (RBCs). Inhibition of agglutination by blood-type specific antisera (anti-D) was employed to evaluate the effect of the polymer coating. The remaining single cells after incubation with anti-D sera were counted using a simple hemocytometer (Improved Neubauer Ruling). The extent of surface coating was evaluated by addition of FITC labeled-anti-D to the cells and recording the fluorescence intensity ratio of FITC-anti-D bound cells of the PEG-RBCs versus control (uncoated) RBCs. The morphology of RBCs was evaluated by scanning electron microscopy (SEM). The effect of polymer coating, based on the immunological response of RBCs, using two kinds of activated mPEG, at optimum conditions of PEGylation was compared. It was found that succinimidyl carbonate at its optimum condition (pH=8.7, temperature =14°C and reaction time =60 min) is more effective than cyanuric chloride at its optimum condition (pH=8.7, temperature =14 °C and reaction time =30 min) for RBC coating with mPEG. |
| first_indexed | 2024-04-13T02:20:26Z |
| format | Article |
| id | doaj.art-a384844b8e9e44b4a7035c2acaccf0c1 |
| institution | Directory Open Access Journal |
| issn | 1021-9986 1021-9986 |
| language | English |
| last_indexed | 2024-04-13T02:20:26Z |
| publishDate | 2008-09-01 |
| publisher | Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
| record_format | Article |
| series | Iranian Journal of Chemistry & Chemical Engineering |
| spelling | doaj.art-a384844b8e9e44b4a7035c2acaccf0c12022-12-22T03:06:59ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99862008-09-01273196923Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different ReagentsFatemeh Sarvi0Sameereh Hashemi Najafabadi1Ebrahim Vasheghani Farahani2Seyed Abbas Shojaosadati3Biotechnology Group, Department of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-143 Tehran, I.R. IRANBiotechnology Group, Department of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-143 Tehran, I.R. IRANBiotechnology Group, Department of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-143 Tehran, I.R. IRANBiotechnology Group, Department of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-143 Tehran, I.R. IRANMethoxy poly(ethylene glycol) (mPEG) with molecular mass of 5 kDa activated with succinimidyl carbonate and cyanuric chloride, separately was covalently attached to human red blood cells (RBCs). Inhibition of agglutination by blood-type specific antisera (anti-D) was employed to evaluate the effect of the polymer coating. The remaining single cells after incubation with anti-D sera were counted using a simple hemocytometer (Improved Neubauer Ruling). The extent of surface coating was evaluated by addition of FITC labeled-anti-D to the cells and recording the fluorescence intensity ratio of FITC-anti-D bound cells of the PEG-RBCs versus control (uncoated) RBCs. The morphology of RBCs was evaluated by scanning electron microscopy (SEM). The effect of polymer coating, based on the immunological response of RBCs, using two kinds of activated mPEG, at optimum conditions of PEGylation was compared. It was found that succinimidyl carbonate at its optimum condition (pH=8.7, temperature =14°C and reaction time =60 min) is more effective than cyanuric chloride at its optimum condition (pH=8.7, temperature =14 °C and reaction time =30 min) for RBC coating with mPEG.http://www.ijcce.ac.ir/article_6923_195a6340a2016640d1810451c86a8c01.pdfred blood cellsmethoxy poly(ethylene glycol)succinimidyl carbonatecyanuric chlorideanti-d sera |
| spellingShingle | Fatemeh Sarvi Sameereh Hashemi Najafabadi Ebrahim Vasheghani Farahani Seyed Abbas Shojaosadati Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different Reagents Iranian Journal of Chemistry & Chemical Engineering red blood cells methoxy poly(ethylene glycol) succinimidyl carbonate cyanuric chloride anti-d sera |
| title | Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different Reagents |
| title_full | Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different Reagents |
| title_fullStr | Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different Reagents |
| title_full_unstemmed | Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different Reagents |
| title_short | Surface Coating of Red Blood Cells with Monomethoxy poly(ethylene glycol) Activated with Two Different Reagents |
| title_sort | surface coating of red blood cells with monomethoxy poly ethylene glycol activated with two different reagents |
| topic | red blood cells methoxy poly(ethylene glycol) succinimidyl carbonate cyanuric chloride anti-d sera |
| url | http://www.ijcce.ac.ir/article_6923_195a6340a2016640d1810451c86a8c01.pdf |
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