Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm
Cooling goat sperm insemination doses to 4 °C causes a delay in their delivery. However, chilling these doses during the transportation period could expedite their delivery and the insemination process. In this study, an economical and simple apparatus for chilling goat semen doses in itinere was de...
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MDPI AG
2020-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/12/2399 |
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| author | Eva Mocé Salvador A. Lozano-Palazón María del Mar Martínez-Granell María Lorena Mocé Ernesto A. Gómez |
| author_facet | Eva Mocé Salvador A. Lozano-Palazón María del Mar Martínez-Granell María Lorena Mocé Ernesto A. Gómez |
| author_sort | Eva Mocé |
| collection | DOAJ |
| description | Cooling goat sperm insemination doses to 4 °C causes a delay in their delivery. However, chilling these doses during the transportation period could expedite their delivery and the insemination process. In this study, an economical and simple apparatus for chilling goat semen doses in itinere was developed, and the in vitro quality and in vivo fertility of these doses were compared with those chilled by means of a programmable water bath in the laboratory at a rate of −0.18 °C/min. Of the tested prototypes, the one that provided an optimal combination of the chilling rate (average of −0.09 °C/min) and time required to reach 4 °C (3 h 45 min) was selected for further testing. Immediately after chilling and 24 h later, the doses chilled in the prototype were determined to be of higher quality than the samples chilled in the programmable water bath. Finally, the kidding rate was similar between the doses chilled in the programmable water bath (61.7% ± 7.1%) and in the prototype (56.1% ± 5.9%). In conclusion, successful chilling of goat sperm doses during transport is possible, thereby accelerating the delivery of insemination doses. |
| first_indexed | 2024-03-10T14:03:09Z |
| format | Article |
| id | doaj.art-a38624989b034203a5c41452bce4b1e9 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T14:03:09Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-a38624989b034203a5c41452bce4b1e92023-11-21T00:55:33ZengMDPI AGAnimals2076-26152020-12-011012239910.3390/ani10122399Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck SpermEva Mocé0Salvador A. Lozano-Palazón1María del Mar Martínez-Granell2María Lorena Mocé3Ernesto A. Gómez4CITA—Instituto Valenciano de Investigaciones Agrarias, Polígono la Esperanza, 100, 12400 Segorbe, Castellón, SpainACRIMUR, C/Baron del Solar, 22-A, Edificio II, Entresuelo A, Puerta B, 30520 Jumilla, Murcia, SpainCITA—Instituto Valenciano de Investigaciones Agrarias, Polígono la Esperanza, 100, 12400 Segorbe, Castellón, SpainDepartment of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, C/Tirant lo Blanc, 7, 46113 Alfara del Patriarca, Valencia, SpainCITA—Instituto Valenciano de Investigaciones Agrarias, Polígono la Esperanza, 100, 12400 Segorbe, Castellón, SpainCooling goat sperm insemination doses to 4 °C causes a delay in their delivery. However, chilling these doses during the transportation period could expedite their delivery and the insemination process. In this study, an economical and simple apparatus for chilling goat semen doses in itinere was developed, and the in vitro quality and in vivo fertility of these doses were compared with those chilled by means of a programmable water bath in the laboratory at a rate of −0.18 °C/min. Of the tested prototypes, the one that provided an optimal combination of the chilling rate (average of −0.09 °C/min) and time required to reach 4 °C (3 h 45 min) was selected for further testing. Immediately after chilling and 24 h later, the doses chilled in the prototype were determined to be of higher quality than the samples chilled in the programmable water bath. Finally, the kidding rate was similar between the doses chilled in the programmable water bath (61.7% ± 7.1%) and in the prototype (56.1% ± 5.9%). In conclusion, successful chilling of goat sperm doses during transport is possible, thereby accelerating the delivery of insemination doses.https://www.mdpi.com/2076-2615/10/12/2399coolingchillinggoat bucksemenliquid-stored semenrefrigerated semen |
| spellingShingle | Eva Mocé Salvador A. Lozano-Palazón María del Mar Martínez-Granell María Lorena Mocé Ernesto A. Gómez Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm Animals cooling chilling goat buck semen liquid-stored semen refrigerated semen |
| title | Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm |
| title_full | Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm |
| title_fullStr | Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm |
| title_full_unstemmed | Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm |
| title_short | Effect of the Refrigeration System on In Vitro Quality and In Vivo Fertility of Goat Buck Sperm |
| title_sort | effect of the refrigeration system on in vitro quality and in vivo fertility of goat buck sperm |
| topic | cooling chilling goat buck semen liquid-stored semen refrigerated semen |
| url | https://www.mdpi.com/2076-2615/10/12/2399 |
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