Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum
In rhizospheres, chemical barrier-forming natural compounds play a key role in preventing pathogenic bacteria from infecting plant roots. Here, we sought to identify specific phenolic exudates in tobacco (Nicotiana tobaccum) plants infected by the soil-borne pathogen Ralstonia solanacearum that may...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-08-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.690586/full |
| _version_ | 1819128644984897536 |
|---|---|
| author | Shili Li Jing Pi Hongjiang Zhu Liang Yang Xingguo Zhang Wei Ding |
| author_facet | Shili Li Jing Pi Hongjiang Zhu Liang Yang Xingguo Zhang Wei Ding |
| author_sort | Shili Li |
| collection | DOAJ |
| description | In rhizospheres, chemical barrier-forming natural compounds play a key role in preventing pathogenic bacteria from infecting plant roots. Here, we sought to identify specific phenolic exudates in tobacco (Nicotiana tobaccum) plants infected by the soil-borne pathogen Ralstonia solanacearum that may exhibit antibacterial activity and promote plant resistance against pathogens. Among detected phenolic acids, only caffeic acid was significantly induced in infected plants by R. solanacearum relative to healthy plants, and the concentration of caffeic acid reached 1.95 μg/mL. In vivo, caffeic acid at 200 μg/mL was highly active against R. solanacearum and obviously damaged the membrane structure of the R. solanacearum cells, resulting in the thinning of the cell membrane and irregular cavities in cells. Moreover, caffeic acid significantly inhibited biofilm formation by repressing the expression of the lecM and epsE genes. In vitro, caffeic acid could effectively activate phenylalanine ammonia-lyase (PAL) and peroxidase (POD) and promote the accumulation of lignin and hydroxyproline. In pot and field experiments, exogenous applications of caffeic acid significantly reduced and delayed the incidence of tobacco bacterial wilt. Taken together, all these results suggest that caffeic acid played a crucial role in defending against R. solanacearum infection and was a potential and effective antibacterial agent for controlling bacterial wilt. |
| first_indexed | 2024-12-22T08:31:06Z |
| format | Article |
| id | doaj.art-a387e966aa724737ba44ebdab55115a8 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T08:31:06Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a387e966aa724737ba44ebdab55115a82022-12-21T18:32:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-08-011210.3389/fpls.2021.690586690586Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearumShili Li0Jing Pi1Hongjiang Zhu2Liang Yang3Xingguo Zhang4Wei Ding5Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing, ChinaLaboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing, ChinaLaboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing, ChinaLaboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing, ChinaKey Laboratory of Horticulture Science for Southern Mountainous Regions, Southwest University, Chongqing, ChinaLaboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing, ChinaIn rhizospheres, chemical barrier-forming natural compounds play a key role in preventing pathogenic bacteria from infecting plant roots. Here, we sought to identify specific phenolic exudates in tobacco (Nicotiana tobaccum) plants infected by the soil-borne pathogen Ralstonia solanacearum that may exhibit antibacterial activity and promote plant resistance against pathogens. Among detected phenolic acids, only caffeic acid was significantly induced in infected plants by R. solanacearum relative to healthy plants, and the concentration of caffeic acid reached 1.95 μg/mL. In vivo, caffeic acid at 200 μg/mL was highly active against R. solanacearum and obviously damaged the membrane structure of the R. solanacearum cells, resulting in the thinning of the cell membrane and irregular cavities in cells. Moreover, caffeic acid significantly inhibited biofilm formation by repressing the expression of the lecM and epsE genes. In vitro, caffeic acid could effectively activate phenylalanine ammonia-lyase (PAL) and peroxidase (POD) and promote the accumulation of lignin and hydroxyproline. In pot and field experiments, exogenous applications of caffeic acid significantly reduced and delayed the incidence of tobacco bacterial wilt. Taken together, all these results suggest that caffeic acid played a crucial role in defending against R. solanacearum infection and was a potential and effective antibacterial agent for controlling bacterial wilt.https://www.frontiersin.org/articles/10.3389/fpls.2021.690586/fullcaffeic acidphenolic acidsantibacterial activitytobacco bacterial wiltRalstonia solanacearum |
| spellingShingle | Shili Li Jing Pi Hongjiang Zhu Liang Yang Xingguo Zhang Wei Ding Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum Frontiers in Plant Science caffeic acid phenolic acids antibacterial activity tobacco bacterial wilt Ralstonia solanacearum |
| title | Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum |
| title_full | Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum |
| title_fullStr | Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum |
| title_full_unstemmed | Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum |
| title_short | Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum |
| title_sort | caffeic acid in tobacco root exudate defends tobacco plants from infection by ralstonia solanacearum |
| topic | caffeic acid phenolic acids antibacterial activity tobacco bacterial wilt Ralstonia solanacearum |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.690586/full |
| work_keys_str_mv | AT shilili caffeicacidintobaccorootexudatedefendstobaccoplantsfrominfectionbyralstoniasolanacearum AT jingpi caffeicacidintobaccorootexudatedefendstobaccoplantsfrominfectionbyralstoniasolanacearum AT hongjiangzhu caffeicacidintobaccorootexudatedefendstobaccoplantsfrominfectionbyralstoniasolanacearum AT liangyang caffeicacidintobaccorootexudatedefendstobaccoplantsfrominfectionbyralstoniasolanacearum AT xingguozhang caffeicacidintobaccorootexudatedefendstobaccoplantsfrominfectionbyralstoniasolanacearum AT weiding caffeicacidintobaccorootexudatedefendstobaccoplantsfrominfectionbyralstoniasolanacearum |