Development of an electrode immunosensor using carbon nanofibres-gold nanoparticles-mercaptopropionic acid-polyethylenimine for chicken liver containing colistin
Colistin is not approved for use by humans; however, it has been used in veterinary medicine and as an additive in animal feeds for many years. This study aims to develop an amperometric immunosensor to detect colistin in chicken liver. An anti-colistin antibody was immobilised on the screen-printed...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Agriculture and Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324000279 |
| _version_ | 1797271269580734464 |
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| author | Harsh Kumar Dinesh Kumar Marian Valko Sivakumar Manickam Suliman Y. Alomar Natália Cruz-Martins Kamil Kuča |
| author_facet | Harsh Kumar Dinesh Kumar Marian Valko Sivakumar Manickam Suliman Y. Alomar Natália Cruz-Martins Kamil Kuča |
| author_sort | Harsh Kumar |
| collection | DOAJ |
| description | Colistin is not approved for use by humans; however, it has been used in veterinary medicine and as an additive in animal feeds for many years. This study aims to develop an amperometric immunosensor to detect colistin in chicken liver. An anti-colistin antibody was immobilised on the screen-printed electrode's surface using CNF/AuNPs/MPA/PEI to develop the immunosensor. With the help of CV measurements, this sensor was used to analyse the electrical and chemical reaction of colistin after being injected into chicken liver. Furthermore, FE-SEM and FTIR characterized the electrode surface throughout the fabrication process. Based on CV analysis, 0.072 μgkg-1 LOD was calculated with R2 value of 0.995. The results of conventional methods were compared to liver samples obtained from commercial sources to validate the immunosensor. In the constructed immunosensor, colistin had a high level of specificity, stable for more than six months despite an 11 % decline in CV current. It is imperative to highlight that the present study did not include a process of recovering colistin drug from liver tissue. This lack constitutes a constraint of our research and is annotated with suggestions for future work. |
| first_indexed | 2024-03-07T14:00:18Z |
| format | Article |
| id | doaj.art-a38820655b94496da2b5423fcbde81e6 |
| institution | Directory Open Access Journal |
| issn | 2666-1543 |
| language | English |
| last_indexed | 2024-03-07T14:00:18Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj.art-a38820655b94496da2b5423fcbde81e62024-03-07T05:29:49ZengElsevierJournal of Agriculture and Food Research2666-15432024-03-0115100990Development of an electrode immunosensor using carbon nanofibres-gold nanoparticles-mercaptopropionic acid-polyethylenimine for chicken liver containing colistinHarsh Kumar0Dinesh Kumar1Marian Valko2Sivakumar Manickam3Suliman Y. Alomar4Natália Cruz-Martins5Kamil Kuča6School of Bioengineering & Food Technology, Shoolini University of Biotechnology and Management Sciences, Solan, 173229, India; Centre of Advanced Technologies, Faculty of Science, University of Hradec Kralove, Rokitanskeho 62, 50003, Hradec Kralove, Czech RepublicSchool of Bioengineering & Food Technology, Shoolini University of Biotechnology and Management Sciences, Solan, 173229, India; Corresponding author.Institute of Physical Chemistry and Chemical Physics, Faculty of Chemical and Food Technology, Slovak University of Technology, 81237, Bratislava, Slovakia; King Saud University, Zoology Department, College of Science, Riyadh, 11451, Saudi ArabiaPetroleum and Chemical Engineering, Faculty of Engineering, Universiti Teknologi Brunei, Bandar Seri Begawan, BE1410, BruneiKing Saud University, Zoology Department, College of Science, Riyadh, 11451, Saudi ArabiaDepartment of Biomedicine, Faculty of Medicine, University of Porto, 4200-319, Porto, Portugal; Institute for Research and Innovation in Health (i3S), University of Porto, 4200-135, Porto, Portugal; Institute of Research and Advanced Training in Health Sciences and Technologies (CESPU), Rua Central de Gandra, 1317, 4585-116, Gandra PRD, PortugalDepartment of Chemistry, Faculty of Science, University of Hradec Kralove, 50003, Hradec Kralove, Czech Republic; Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic; Corresponding author. Department of Chemistry, Faculty of Science, University of Hradec Kralove, 50003, Hradec Kralove, Czech Republic.Colistin is not approved for use by humans; however, it has been used in veterinary medicine and as an additive in animal feeds for many years. This study aims to develop an amperometric immunosensor to detect colistin in chicken liver. An anti-colistin antibody was immobilised on the screen-printed electrode's surface using CNF/AuNPs/MPA/PEI to develop the immunosensor. With the help of CV measurements, this sensor was used to analyse the electrical and chemical reaction of colistin after being injected into chicken liver. Furthermore, FE-SEM and FTIR characterized the electrode surface throughout the fabrication process. Based on CV analysis, 0.072 μgkg-1 LOD was calculated with R2 value of 0.995. The results of conventional methods were compared to liver samples obtained from commercial sources to validate the immunosensor. In the constructed immunosensor, colistin had a high level of specificity, stable for more than six months despite an 11 % decline in CV current. It is imperative to highlight that the present study did not include a process of recovering colistin drug from liver tissue. This lack constitutes a constraint of our research and is annotated with suggestions for future work.http://www.sciencedirect.com/science/article/pii/S2666154324000279ImmunosensorChickenColistinHealthToxicity |
| spellingShingle | Harsh Kumar Dinesh Kumar Marian Valko Sivakumar Manickam Suliman Y. Alomar Natália Cruz-Martins Kamil Kuča Development of an electrode immunosensor using carbon nanofibres-gold nanoparticles-mercaptopropionic acid-polyethylenimine for chicken liver containing colistin Journal of Agriculture and Food Research Immunosensor Chicken Colistin Health Toxicity |
| title | Development of an electrode immunosensor using carbon nanofibres-gold nanoparticles-mercaptopropionic acid-polyethylenimine for chicken liver containing colistin |
| title_full | Development of an electrode immunosensor using carbon nanofibres-gold nanoparticles-mercaptopropionic acid-polyethylenimine for chicken liver containing colistin |
| title_fullStr | Development of an electrode immunosensor using carbon nanofibres-gold nanoparticles-mercaptopropionic acid-polyethylenimine for chicken liver containing colistin |
| title_full_unstemmed | Development of an electrode immunosensor using carbon nanofibres-gold nanoparticles-mercaptopropionic acid-polyethylenimine for chicken liver containing colistin |
| title_short | Development of an electrode immunosensor using carbon nanofibres-gold nanoparticles-mercaptopropionic acid-polyethylenimine for chicken liver containing colistin |
| title_sort | development of an electrode immunosensor using carbon nanofibres gold nanoparticles mercaptopropionic acid polyethylenimine for chicken liver containing colistin |
| topic | Immunosensor Chicken Colistin Health Toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324000279 |
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