Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method
<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p>The study was aimed to evaluate the efficacy of formalin-killed no...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Asosiasi Sains Akuakultur Indonesia
2015-05-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | http://journal.ipb.ac.id/index.php/jai/article/view/9458 |
| _version_ | 1819016535413358592 |
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| author | , Sukenda Trian Rizky Febriansyah Sri Nuryati |
| author_facet | , Sukenda Trian Rizky Febriansyah Sri Nuryati |
| author_sort | , Sukenda |
| collection | DOAJ |
| description | <p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p>The study was aimed to evaluate the efficacy of formalin-killed non-hemolytic <em>Streptococcus agalactiae </em>N14G and NK1 isolates whole-killed vaccine to prevent streptococcosis in tilapia. Ten fishes were reared in a tank 60x30x35 cm3 with an average body weight at 10.79±0.99 g. Fish was vaccinated through bath immersion at a concentration of 109 cfu/mL. Fish was subsequently challenged by intraperitonial injection of <em>Streptococcus agalactiae </em>105 cfu/mL at 11 days post-vaccination. Parameters observed were survival, relative percent survival (RPS), total leukocyte, phagocytic activity, antibody titer, total erythrocyte, haemoglobin level, haematocrit level, dan water quality. Samplings were performed in day-0, 20, and 30 after vaccination. Both vaccines have shown higher survival (60%) and RPS (40%) when challenged with pathogenic <em>Streptococcus </em>N14G isolates than other treatments. Based on RPS percentage observed, those vaccine were still not sufficiently effective to combat <em>S. agalactiae </em>infection.</p><p> </p><p>Keywords: tilapia, bath immersion, <em>Streptococcus agalactiae</em>, whole-cell vaccine</p><br /><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong><br /></strong></p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p>Penelitian ini bertujuan untuk mengevaluasi efikasi vaksin <em>formalin-killed cell Streptococcus agalactiae </em>tipe isolat nonhemolitik N14G dan NK1 se utuh yang diberikan melalui perendaman dalam mencegah penyakit streptococcosis pada ikan nila. Ikan nila yang digunakan memiliki bobot 10,79±0,99 g, dipelihara sebanyak sepuluh ekor dalam akuarium ukuran 60x30x35 cm3. Ikan divaksinasi dengan metode perendaman dengan dosis 109 cfu/mL. Uji tantang dilakukan pada hari ke-11 pascavaksinasi dengan dosis 105 cfu/mL. Parameter yang diamati meliputi sintasan (SR), sintasan relatif/<em>relative percent surviva</em>l (RPS), total leukosit, aktivitas fagositik, titer antibodi, total eritrosit, kadar hemoglobin, kadar hematokrit, dan kualitas air. Pengamatan parameter dilakukan pada hari ke-0, ke-10, ke-20, dan ke-30. Hasil penelitian menunjukkan perlakuan kedua vaksin yang diinfeksi bakteri patogen isolat N14G memberikan nilai sintasan dan nilai RPS tertinggi dibanding perlakuan lainnya. Nilai sintasan dan RPS kedua perlakuan tersebut adalah 60% dan 40%. Nilai RPS yang cukup kecil menunjukkan vaksin yang diberikan masih kurang efektif untuk mencegah infeksi bakteri <em>S. agalactiae</em>.</p><p> </p><p>Kata kunci: ikan nila, perendaman, <em>Streptococcus agalactiae</em>, vaksin sel utuh</p> |
| first_indexed | 2024-12-21T02:49:10Z |
| format | Article |
| id | doaj.art-a38b10a5269c4151b695ab9c4881c050 |
| institution | Directory Open Access Journal |
| issn | 1412-5269 2354-6700 |
| language | English |
| last_indexed | 2024-12-21T02:49:10Z |
| publishDate | 2015-05-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj.art-a38b10a5269c4151b695ab9c4881c0502022-12-21T19:18:29ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-05-01131839310.19027/jai.13.83-937899Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method, Sukenda0Trian Rizky Febriansyah1Sri Nuryati2Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p>The study was aimed to evaluate the efficacy of formalin-killed non-hemolytic <em>Streptococcus agalactiae </em>N14G and NK1 isolates whole-killed vaccine to prevent streptococcosis in tilapia. Ten fishes were reared in a tank 60x30x35 cm3 with an average body weight at 10.79±0.99 g. Fish was vaccinated through bath immersion at a concentration of 109 cfu/mL. Fish was subsequently challenged by intraperitonial injection of <em>Streptococcus agalactiae </em>105 cfu/mL at 11 days post-vaccination. Parameters observed were survival, relative percent survival (RPS), total leukocyte, phagocytic activity, antibody titer, total erythrocyte, haemoglobin level, haematocrit level, dan water quality. Samplings were performed in day-0, 20, and 30 after vaccination. Both vaccines have shown higher survival (60%) and RPS (40%) when challenged with pathogenic <em>Streptococcus </em>N14G isolates than other treatments. Based on RPS percentage observed, those vaccine were still not sufficiently effective to combat <em>S. agalactiae </em>infection.</p><p> </p><p>Keywords: tilapia, bath immersion, <em>Streptococcus agalactiae</em>, whole-cell vaccine</p><br /><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong><br /></strong></p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p>Penelitian ini bertujuan untuk mengevaluasi efikasi vaksin <em>formalin-killed cell Streptococcus agalactiae </em>tipe isolat nonhemolitik N14G dan NK1 se utuh yang diberikan melalui perendaman dalam mencegah penyakit streptococcosis pada ikan nila. Ikan nila yang digunakan memiliki bobot 10,79±0,99 g, dipelihara sebanyak sepuluh ekor dalam akuarium ukuran 60x30x35 cm3. Ikan divaksinasi dengan metode perendaman dengan dosis 109 cfu/mL. Uji tantang dilakukan pada hari ke-11 pascavaksinasi dengan dosis 105 cfu/mL. Parameter yang diamati meliputi sintasan (SR), sintasan relatif/<em>relative percent surviva</em>l (RPS), total leukosit, aktivitas fagositik, titer antibodi, total eritrosit, kadar hemoglobin, kadar hematokrit, dan kualitas air. Pengamatan parameter dilakukan pada hari ke-0, ke-10, ke-20, dan ke-30. Hasil penelitian menunjukkan perlakuan kedua vaksin yang diinfeksi bakteri patogen isolat N14G memberikan nilai sintasan dan nilai RPS tertinggi dibanding perlakuan lainnya. Nilai sintasan dan RPS kedua perlakuan tersebut adalah 60% dan 40%. Nilai RPS yang cukup kecil menunjukkan vaksin yang diberikan masih kurang efektif untuk mencegah infeksi bakteri <em>S. agalactiae</em>.</p><p> </p><p>Kata kunci: ikan nila, perendaman, <em>Streptococcus agalactiae</em>, vaksin sel utuh</p>http://journal.ipb.ac.id/index.php/jai/article/view/9458 |
| spellingShingle | , Sukenda Trian Rizky Febriansyah Sri Nuryati Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method Jurnal Akuakultur Indonesia |
| title | Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method |
| title_full | Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method |
| title_fullStr | Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method |
| title_full_unstemmed | Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method |
| title_short | Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method |
| title_sort | whole cell vaccine of streptococcus agalactiae in oreochromis sp with immersion method |
| url | http://journal.ipb.ac.id/index.php/jai/article/view/9458 |
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