Guanosine-5′-triphosphate cyclohydrolase 1 regulated long noncoding RNAs are potential targets for microglial activation in neuropathic pain
Several studies have confirmed that microglia are involved in neuropathic pain. Inhibition of guanosine-5′-triphosphate cyclohydrolase 1 (GTPCH1) can reduce the inflammation of microglia. However, the precise mechanism by which GTPCH1 regulates neuropathic pain remains unclear. In this study, BV2 mi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Neural Regeneration Research |
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| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=3;spage=596;epage=600;aulast=Liang |
| _version_ | 1818130572538544128 |
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| author | Yan-Hu Liang Guo-Wu Chen Xue-Song Li Shu Jia Chun-Yang Meng |
| author_facet | Yan-Hu Liang Guo-Wu Chen Xue-Song Li Shu Jia Chun-Yang Meng |
| author_sort | Yan-Hu Liang |
| collection | DOAJ |
| description | Several studies have confirmed that microglia are involved in neuropathic pain. Inhibition of guanosine-5′-triphosphate cyclohydrolase 1 (GTPCH1) can reduce the inflammation of microglia. However, the precise mechanism by which GTPCH1 regulates neuropathic pain remains unclear. In this study, BV2 microglia were transfected with adenovirus to knockdown GTPCH1 expression. High throughput sequencing analysis revealed that the mitogen-activated protein kinase (MAPK) related pathways and proteins were the most significantly down-regulated molecular function. Co-expression network analysis of Mapk14 mRNA and five long noncoding RNAs (lncRNAs) revealed their correlation. Quantitative reverse transcription-polymerase chain reaction revealed that among five lncRNAs, ENSMUST00000205634, ENSMUST00000218450 and ENSMUST00000156079 were related to the downregulation of Mapk14 mRNA expression. These provide some new potential targets for the involvement of GTPCH1 in neuropathic pain. This study is the first to note the differential expression of lncRNAs and mRNA in GTPCH1 knockdown BV2 microglia. Findings from this study reveal the mechanism by which GTPCH1 activates microglia and provide new potential targets for microglial activation in neuropathic pain. |
| first_indexed | 2024-12-11T08:07:10Z |
| format | Article |
| id | doaj.art-a38c30ac91ed4c3aa4a7a5799c002352 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-11T08:07:10Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-a38c30ac91ed4c3aa4a7a5799c0023522022-12-22T01:14:58ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742021-01-0116359660010.4103/1673-5374.290914Guanosine-5′-triphosphate cyclohydrolase 1 regulated long noncoding RNAs are potential targets for microglial activation in neuropathic painYan-Hu LiangGuo-Wu ChenXue-Song LiShu JiaChun-Yang MengSeveral studies have confirmed that microglia are involved in neuropathic pain. Inhibition of guanosine-5′-triphosphate cyclohydrolase 1 (GTPCH1) can reduce the inflammation of microglia. However, the precise mechanism by which GTPCH1 regulates neuropathic pain remains unclear. In this study, BV2 microglia were transfected with adenovirus to knockdown GTPCH1 expression. High throughput sequencing analysis revealed that the mitogen-activated protein kinase (MAPK) related pathways and proteins were the most significantly down-regulated molecular function. Co-expression network analysis of Mapk14 mRNA and five long noncoding RNAs (lncRNAs) revealed their correlation. Quantitative reverse transcription-polymerase chain reaction revealed that among five lncRNAs, ENSMUST00000205634, ENSMUST00000218450 and ENSMUST00000156079 were related to the downregulation of Mapk14 mRNA expression. These provide some new potential targets for the involvement of GTPCH1 in neuropathic pain. This study is the first to note the differential expression of lncRNAs and mRNA in GTPCH1 knockdown BV2 microglia. Findings from this study reveal the mechanism by which GTPCH1 activates microglia and provide new potential targets for microglial activation in neuropathic pain.http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=3;spage=596;epage=600;aulast=Liangcells; factors; inflammation; microglia; pain; pathway; protein; rna |
| spellingShingle | Yan-Hu Liang Guo-Wu Chen Xue-Song Li Shu Jia Chun-Yang Meng Guanosine-5′-triphosphate cyclohydrolase 1 regulated long noncoding RNAs are potential targets for microglial activation in neuropathic pain Neural Regeneration Research cells; factors; inflammation; microglia; pain; pathway; protein; rna |
| title | Guanosine-5′-triphosphate cyclohydrolase 1 regulated long noncoding RNAs are potential targets for microglial activation in neuropathic pain |
| title_full | Guanosine-5′-triphosphate cyclohydrolase 1 regulated long noncoding RNAs are potential targets for microglial activation in neuropathic pain |
| title_fullStr | Guanosine-5′-triphosphate cyclohydrolase 1 regulated long noncoding RNAs are potential targets for microglial activation in neuropathic pain |
| title_full_unstemmed | Guanosine-5′-triphosphate cyclohydrolase 1 regulated long noncoding RNAs are potential targets for microglial activation in neuropathic pain |
| title_short | Guanosine-5′-triphosphate cyclohydrolase 1 regulated long noncoding RNAs are potential targets for microglial activation in neuropathic pain |
| title_sort | guanosine 5 triphosphate cyclohydrolase 1 regulated long noncoding rnas are potential targets for microglial activation in neuropathic pain |
| topic | cells; factors; inflammation; microglia; pain; pathway; protein; rna |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=3;spage=596;epage=600;aulast=Liang |
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