Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia
Background and Aim: Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This bacterium is treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene in K. pneumoniae may be transferred to other bacteria. One of the known resistance g...
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| Format: | Article |
| Language: | English |
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Veterinary World
2019-04-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.12/April-2019/14.pdf |
| _version_ | 1819144899507781632 |
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| author | Meutia Hayati Agustin Indrawati Ni Luh Putu Ika Mayasari Istiyaningsih Istiyaningsih Neneng Atikah |
| author_facet | Meutia Hayati Agustin Indrawati Ni Luh Putu Ika Mayasari Istiyaningsih Istiyaningsih Neneng Atikah |
| author_sort | Meutia Hayati |
| collection | DOAJ |
| description | Background and Aim: Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This bacterium is treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene in K. pneumoniae may be transferred to other bacteria. One of the known resistance genes is extended-spectrum β-lactamase (ESBL). This research aimed to study K. pneumoniae isolated from chicken farms in East Java, Indonesia, by observing the antibiotic resistance pattern and detect the presence of ESBL coding gene within the isolates.
Materials and Methods: A total of 11 K. pneumoniae isolates were collected from 141 chicken cloacal swabs from two regencies in East Java. All isolates were identified using the polymerase chain reaction method. Antimicrobial susceptibility was determined by agar dilution method on identified isolates, which then processed for molecular characterization to detect ESBL coding gene within the K. pneumoniae isolates found.
Results: The result of antibiotic sensitivity test in 11 isolates showed highest antibiotic resistance level toward ampicillin, amoxicillin, and oxytetracycline (100%, 100%, and 90.9%) and still sensitive to gentamicin. Resistance against colistin, doxycycline, ciprofloxacin, and enrofloxacin is varied by 90.9%, 54.5%, 27.3%, and 18.2%, respectively. All isolates of K. pneumoniae were classified as multidrug resistance (MDR) bacteria. Resistance gene analysis revealed the isolates harbored as blaSHV (9.1%), blaTEM (100%), and blaCTX-M (90.9%).
Conclusion: All the bacterial isolates were classified as MDR bacteria and harbored two of the transmissible ESBL genes. The presence of antibiotic resistance genes in bacteria has the potential to spread its resistance properties. |
| first_indexed | 2024-12-22T12:49:28Z |
| format | Article |
| id | doaj.art-a38c871d127b488985f00c1452d11492 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-22T12:49:28Z |
| publishDate | 2019-04-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-a38c871d127b488985f00c1452d114922022-12-21T18:25:15ZengVeterinary WorldVeterinary World0972-89882231-09162019-04-0112457858310.14202/vetworld.2019.578-583Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, IndonesiaMeutia Hayati0Agustin Indrawati1Ni Luh Putu Ika Mayasari2Istiyaningsih Istiyaningsih3Neneng Atikah4Division of Medical Microbiology, Faculty of Veterinary Medicine, Bogor Agricultural University-West Java, Indonesia; Division of Bacteriology, National Veterinary Drug Assay Laboratory, Gunungsindur Bogor-West Java, Indonesia.Division of Medical Microbiology, Faculty of Veterinary Medicine, Bogor Agricultural University-West Java, Indonesia.Division of Medical Microbiology, Faculty of Veterinary Medicine, Bogor Agricultural University-West Java, Indonesia.Division of Bacteriology, National Veterinary Drug Assay Laboratory, Gunungsindur Bogor-West Java, Indonesia.Division of Bacteriology, National Veterinary Drug Assay Laboratory, Gunungsindur Bogor-West Java, Indonesia.Background and Aim: Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This bacterium is treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene in K. pneumoniae may be transferred to other bacteria. One of the known resistance genes is extended-spectrum β-lactamase (ESBL). This research aimed to study K. pneumoniae isolated from chicken farms in East Java, Indonesia, by observing the antibiotic resistance pattern and detect the presence of ESBL coding gene within the isolates. Materials and Methods: A total of 11 K. pneumoniae isolates were collected from 141 chicken cloacal swabs from two regencies in East Java. All isolates were identified using the polymerase chain reaction method. Antimicrobial susceptibility was determined by agar dilution method on identified isolates, which then processed for molecular characterization to detect ESBL coding gene within the K. pneumoniae isolates found. Results: The result of antibiotic sensitivity test in 11 isolates showed highest antibiotic resistance level toward ampicillin, amoxicillin, and oxytetracycline (100%, 100%, and 90.9%) and still sensitive to gentamicin. Resistance against colistin, doxycycline, ciprofloxacin, and enrofloxacin is varied by 90.9%, 54.5%, 27.3%, and 18.2%, respectively. All isolates of K. pneumoniae were classified as multidrug resistance (MDR) bacteria. Resistance gene analysis revealed the isolates harbored as blaSHV (9.1%), blaTEM (100%), and blaCTX-M (90.9%). Conclusion: All the bacterial isolates were classified as MDR bacteria and harbored two of the transmissible ESBL genes. The presence of antibiotic resistance genes in bacteria has the potential to spread its resistance properties.http://www.veterinaryworld.org/Vol.12/April-2019/14.pdfantibiotic resistancechickenextended-spectrum β-lactamaseKlebsiella pneumoniae |
| spellingShingle | Meutia Hayati Agustin Indrawati Ni Luh Putu Ika Mayasari Istiyaningsih Istiyaningsih Neneng Atikah Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia Veterinary World antibiotic resistance chicken extended-spectrum β-lactamase Klebsiella pneumoniae |
| title | Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia |
| title_full | Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia |
| title_fullStr | Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia |
| title_full_unstemmed | Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia |
| title_short | Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia |
| title_sort | molecular detection of extended spectrum β lactamase producing klebsiella pneumoniae isolates of chicken origin from east java indonesia |
| topic | antibiotic resistance chicken extended-spectrum β-lactamase Klebsiella pneumoniae |
| url | http://www.veterinaryworld.org/Vol.12/April-2019/14.pdf |
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