The regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase‐2, metalloproteinase‐7, and metalloproteinase‐9 expressions in the rat endometrium
Abstract Purpose We aimed to evaluate how matrix metalloproteinases (MMPs) regulate the trophoblast invasion and placentation. Methods Female rats were divided into the estrous cycle and early pregnancy day groups. Obtained uterine tissues and implantation sites were processed for immunofluorescence...
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| Format: | Article |
| Language: | English |
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Wiley
2020-10-01
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| Series: | Reproductive Medicine and Biology |
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| Online Access: | https://doi.org/10.1002/rmb2.12342 |
| _version_ | 1818878747814658048 |
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| author | Rasim Hamutoğlu Hüseyin Eray Bulut Celal Kaloğlu Ozan Önder Tuğba Dağdeviren Merve Nur Aydemir Ertan Mahir Korkmaz |
| author_facet | Rasim Hamutoğlu Hüseyin Eray Bulut Celal Kaloğlu Ozan Önder Tuğba Dağdeviren Merve Nur Aydemir Ertan Mahir Korkmaz |
| author_sort | Rasim Hamutoğlu |
| collection | DOAJ |
| description | Abstract Purpose We aimed to evaluate how matrix metalloproteinases (MMPs) regulate the trophoblast invasion and placentation. Methods Female rats were divided into the estrous cycle and early pregnancy day groups. Obtained uterine tissues and implantation sites were processed for immunofluorescence and real‐time PCR examinations. Results The mRNA expression of MMP‐7 was higher than MMP‐2 and MMP‐9. Immunofluorescence findings confirmed that MMP‐2, MMP‐7, and MMP‐9 were localized in the endometrial stroma, while MMP‐7 was high in glandular and lining epithelial cells throughout the entire estrous cycle. However, their immunolocalizations and mRNA expressions were dramatically changed with the early pregnancy days. The MMP‐7 reached very strong immunostaining in the giant trophoblast cells (GTCs), and the cytoplasm of mature and differentiating decidual cells, whereas MMP‐2 and MMP‐9 were mostly seen in the primary decidual zone (PDZ), GTCs, and the endothelium of blood vessels. Conclusions All three MMPs seemed likely to be a key mediator of trophoblast invasion into the decidual region as well as angiogenesis during the placentation process. Due to the strong and wide expression of MMP‐7 in the mature decidua, it could be suggested that MMP‐7 is important for decidual ECM remodeling and it might be used as a new marker of decidual reaction. |
| first_indexed | 2024-12-19T14:19:06Z |
| format | Article |
| id | doaj.art-a38d2b2c0fdd432aa362775d80cb4848 |
| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-12-19T14:19:06Z |
| publishDate | 2020-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-a38d2b2c0fdd432aa362775d80cb48482022-12-21T20:17:51ZengWileyReproductive Medicine and Biology1445-57811447-05782020-10-0119438539710.1002/rmb2.12342The regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase‐2, metalloproteinase‐7, and metalloproteinase‐9 expressions in the rat endometriumRasim Hamutoğlu0Hüseyin Eray Bulut1Celal Kaloğlu2Ozan Önder3Tuğba Dağdeviren4Merve Nur Aydemir5Ertan Mahir Korkmaz6Department of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas TurkeyDepartment of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas TurkeyDepartment of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas TurkeyDepartment of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas TurkeyDepartment of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas TurkeyDepartment of Molecular Biology and Genetics Faculty of Science Cumhuriyet University Sivas TurkeyDepartment of Molecular Biology and Genetics Faculty of Science Cumhuriyet University Sivas TurkeyAbstract Purpose We aimed to evaluate how matrix metalloproteinases (MMPs) regulate the trophoblast invasion and placentation. Methods Female rats were divided into the estrous cycle and early pregnancy day groups. Obtained uterine tissues and implantation sites were processed for immunofluorescence and real‐time PCR examinations. Results The mRNA expression of MMP‐7 was higher than MMP‐2 and MMP‐9. Immunofluorescence findings confirmed that MMP‐2, MMP‐7, and MMP‐9 were localized in the endometrial stroma, while MMP‐7 was high in glandular and lining epithelial cells throughout the entire estrous cycle. However, their immunolocalizations and mRNA expressions were dramatically changed with the early pregnancy days. The MMP‐7 reached very strong immunostaining in the giant trophoblast cells (GTCs), and the cytoplasm of mature and differentiating decidual cells, whereas MMP‐2 and MMP‐9 were mostly seen in the primary decidual zone (PDZ), GTCs, and the endothelium of blood vessels. Conclusions All three MMPs seemed likely to be a key mediator of trophoblast invasion into the decidual region as well as angiogenesis during the placentation process. Due to the strong and wide expression of MMP‐7 in the mature decidua, it could be suggested that MMP‐7 is important for decidual ECM remodeling and it might be used as a new marker of decidual reaction.https://doi.org/10.1002/rmb2.12342decidualizationimplantationMMPReal‐time PCRtrophoblast invasion |
| spellingShingle | Rasim Hamutoğlu Hüseyin Eray Bulut Celal Kaloğlu Ozan Önder Tuğba Dağdeviren Merve Nur Aydemir Ertan Mahir Korkmaz The regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase‐2, metalloproteinase‐7, and metalloproteinase‐9 expressions in the rat endometrium Reproductive Medicine and Biology decidualization implantation MMP Real‐time PCR trophoblast invasion |
| title | The regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase‐2, metalloproteinase‐7, and metalloproteinase‐9 expressions in the rat endometrium |
| title_full | The regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase‐2, metalloproteinase‐7, and metalloproteinase‐9 expressions in the rat endometrium |
| title_fullStr | The regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase‐2, metalloproteinase‐7, and metalloproteinase‐9 expressions in the rat endometrium |
| title_full_unstemmed | The regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase‐2, metalloproteinase‐7, and metalloproteinase‐9 expressions in the rat endometrium |
| title_short | The regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase‐2, metalloproteinase‐7, and metalloproteinase‐9 expressions in the rat endometrium |
| title_sort | regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase 2 metalloproteinase 7 and metalloproteinase 9 expressions in the rat endometrium |
| topic | decidualization implantation MMP Real‐time PCR trophoblast invasion |
| url | https://doi.org/10.1002/rmb2.12342 |
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