Transpelvic Magnetic Stimulation Enhances Penile Microvascular Perfusion in a Rat Model: A Novel Interventional Strategy to Prevent Penile Fibrosis after Cavernosal Nerve Injury
Purpose:Purpose: Penile microvascular dysfunction is a known contributor to erectile dysfunction (ED) and penile fibrosis has been shown to impair microvascular perfusion (MVP). Our objectives were to: (i) determine beneficial effects of TPMS to modulate penile MVP, (ii) determine its mechanism, (ii...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Korean Society for Sexual Medicine and Andrology
2022-07-01
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| Series: | The World Journal of Men's Health |
| Subjects: |
| _version_ | 1811344580024467456 |
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| author | Samuel Sorkhi Christopher Cano Sanchez Min Chul Cho Sung Yong Cho Hong Chung Min Gu Park Susan Lahey Tung-Chin Hsieh Valmik Bhargava Mahadevan Raj Rajasekaran |
| author_facet | Samuel Sorkhi Christopher Cano Sanchez Min Chul Cho Sung Yong Cho Hong Chung Min Gu Park Susan Lahey Tung-Chin Hsieh Valmik Bhargava Mahadevan Raj Rajasekaran |
| author_sort | Samuel Sorkhi |
| collection | DOAJ |
| description | Purpose:Purpose: Penile microvascular dysfunction is a known contributor to erectile dysfunction (ED) and penile fibrosis has been shown to impair microvascular perfusion (MVP). Our objectives were to: (i) determine beneficial effects of TPMS to modulate penile MVP, (ii) determine its mechanism, (iii) evaluate impact of cavernosal nerve injury (CNI) on penile MVP, and (iv) deter-mine time-course of cavernosal tissue elastin changes after CNI in rats.
Materials Materials and and Methods:Methods: Adult male rats (n=5) were anesthetized and subjected to TPMS (13%, 15%, and 17%) and MVP changes were recorded using laser speckle contrast imaging (LSCI). Another group of male rats were subjected to either bi-lateral cavernosal nerve injury (CNI; n=7) or sham surgery (n=7). After recovery, animals were monitored for MVP using LSCI before and after TPMS. Rat penile tissues were harvested and analyzed for fibrosis using a marker for elastin.
Results:Results: Rat TPMS resulted in a stimulus dependent increase in MVP; maximal perfusion was observed at 17%. L-N(G)-Nitroarginine methyl ester (L-NAME) resulted in a marked decrease in TPMS induced MVP increase (393.33 AU vs. 210.67 AU). CNI resulted in 40% to 50% decrease in MVP. CNI produced a remarkable increase in elastin deposits that are notice-able throughout the cavernosal tissues post injury.
Conclusions:Conclusions: TPMS is a novel and non-invasive intervention to improve penile MVP after CNI. Potential application includes treatment of ED and sexual function preservation following cancer treatment, possibly through improved penile hemodynam-ics that might help prevent penile hypoxia and fibrosis. |
| first_indexed | 2024-04-13T19:49:26Z |
| format | Article |
| id | doaj.art-a38f6d1311d2486ca317fe1f6451537d |
| institution | Directory Open Access Journal |
| issn | 2287-4208 2287-4690 |
| language | English |
| last_indexed | 2024-04-13T19:49:26Z |
| publishDate | 2022-07-01 |
| publisher | Korean Society for Sexual Medicine and Andrology |
| record_format | Article |
| series | The World Journal of Men's Health |
| spelling | doaj.art-a38f6d1311d2486ca317fe1f6451537d2022-12-22T02:32:36ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902022-07-0140350150810.5534/wjmh.210162Transpelvic Magnetic Stimulation Enhances Penile Microvascular Perfusion in a Rat Model: A Novel Interventional Strategy to Prevent Penile Fibrosis after Cavernosal Nerve Injury Samuel Sorkhi0https://orcid.org/0000-0002-0454-3390 Christopher Cano Sanchez1https://orcid.org/0000-0003-0516-1375Min Chul Cho2https://orcid.org/0000-0001-7288-1706Sung Yong Cho3https://orcid.org/0000-0002-4870-829XHong Chung4https://orcid.org/0000-0002-0151-4965 Min Gu Park5https://orcid.org/0000-0002-4146-3728Susan Lahey6https://orcid.org/0000-0003-1832-7258Tung-Chin Hsieh7https://orcid.org/0000-0002-4149-7610Valmik Bhargava8https://orcid.org/0000-0002-4231-0934Mahadevan Raj Rajasekaran9https://orcid.org/0000-0003-4188-1628Department of Urology, VA San Diego Health Care System, University of California, San Diego, CA, USADepartment of Urology, VA San Diego Health Care System, University of California, San Diego, CA, USADepartment of Urology, SMG-SNU Boramae Medical Center, SeoulDepartment of Urology, Inje University Ilsan Paik Hospital, GoyangDepartment of Urology, Konkuk University School of Medicine, ChungjuDepartment of Urology, Inje University, Seoul Paik Hospital, SeoulDepartment of Urology, VA San Diego Health Care System, University of California, San Diego, CA, USADepartment of Urology, VA San Diego Health Care System, University of California, San Diego, CA, USADepartment of Medicine, VA San Diego Health Care System, University of California, San Diego, CA, USADepartment of Urology, VA San Diego Health Care System, University of California, San Diego, CA, USAPurpose:Purpose: Penile microvascular dysfunction is a known contributor to erectile dysfunction (ED) and penile fibrosis has been shown to impair microvascular perfusion (MVP). Our objectives were to: (i) determine beneficial effects of TPMS to modulate penile MVP, (ii) determine its mechanism, (iii) evaluate impact of cavernosal nerve injury (CNI) on penile MVP, and (iv) deter-mine time-course of cavernosal tissue elastin changes after CNI in rats. Materials Materials and and Methods:Methods: Adult male rats (n=5) were anesthetized and subjected to TPMS (13%, 15%, and 17%) and MVP changes were recorded using laser speckle contrast imaging (LSCI). Another group of male rats were subjected to either bi-lateral cavernosal nerve injury (CNI; n=7) or sham surgery (n=7). After recovery, animals were monitored for MVP using LSCI before and after TPMS. Rat penile tissues were harvested and analyzed for fibrosis using a marker for elastin. Results:Results: Rat TPMS resulted in a stimulus dependent increase in MVP; maximal perfusion was observed at 17%. L-N(G)-Nitroarginine methyl ester (L-NAME) resulted in a marked decrease in TPMS induced MVP increase (393.33 AU vs. 210.67 AU). CNI resulted in 40% to 50% decrease in MVP. CNI produced a remarkable increase in elastin deposits that are notice-able throughout the cavernosal tissues post injury. Conclusions:Conclusions: TPMS is a novel and non-invasive intervention to improve penile MVP after CNI. Potential application includes treatment of ED and sexual function preservation following cancer treatment, possibly through improved penile hemodynam-ics that might help prevent penile hypoxia and fibrosis.elastinerectile dysfunctionfibrosislaser speckle contrast imagingmicrocirculationpenis |
| spellingShingle | Samuel Sorkhi Christopher Cano Sanchez Min Chul Cho Sung Yong Cho Hong Chung Min Gu Park Susan Lahey Tung-Chin Hsieh Valmik Bhargava Mahadevan Raj Rajasekaran Transpelvic Magnetic Stimulation Enhances Penile Microvascular Perfusion in a Rat Model: A Novel Interventional Strategy to Prevent Penile Fibrosis after Cavernosal Nerve Injury The World Journal of Men's Health elastin erectile dysfunction fibrosis laser speckle contrast imaging microcirculation penis |
| title | Transpelvic Magnetic Stimulation Enhances Penile Microvascular Perfusion in a Rat Model: A Novel Interventional Strategy to Prevent Penile Fibrosis after Cavernosal Nerve Injury |
| title_full | Transpelvic Magnetic Stimulation Enhances Penile Microvascular Perfusion in a Rat Model: A Novel Interventional Strategy to Prevent Penile Fibrosis after Cavernosal Nerve Injury |
| title_fullStr | Transpelvic Magnetic Stimulation Enhances Penile Microvascular Perfusion in a Rat Model: A Novel Interventional Strategy to Prevent Penile Fibrosis after Cavernosal Nerve Injury |
| title_full_unstemmed | Transpelvic Magnetic Stimulation Enhances Penile Microvascular Perfusion in a Rat Model: A Novel Interventional Strategy to Prevent Penile Fibrosis after Cavernosal Nerve Injury |
| title_short | Transpelvic Magnetic Stimulation Enhances Penile Microvascular Perfusion in a Rat Model: A Novel Interventional Strategy to Prevent Penile Fibrosis after Cavernosal Nerve Injury |
| title_sort | transpelvic magnetic stimulation enhances penile microvascular perfusion in a rat model a novel interventional strategy to prevent penile fibrosis after cavernosal nerve injury |
| topic | elastin erectile dysfunction fibrosis laser speckle contrast imaging microcirculation penis |
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