NKG2A inhibits TH2 cell effector function <it>in vitro</it>
<p>Abstract</p> <p>Background</p> <p>We previously reported that NKG2A, a key inhibitory ligand for HLA-E, is expressed on activated TH2 but not TH1 cells. Here we measured cytokine expression in human <it>ex vivo </it>TH2 cells upon activation with anti-CD3...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-10-01
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| Series: | BMC Pulmonary Medicine |
| Online Access: | http://www.biomedcentral.com/1471-2466/7/14 |
| _version_ | 1818541931213357056 |
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| author | Mojgani Bahar Freishtat Robert J Nazemzadeh Maryam Nagaraju Kanneboyina Hoffman Eric P |
| author_facet | Mojgani Bahar Freishtat Robert J Nazemzadeh Maryam Nagaraju Kanneboyina Hoffman Eric P |
| author_sort | Mojgani Bahar |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>We previously reported that NKG2A, a key inhibitory ligand for HLA-E, is expressed on activated TH2 but not TH1 cells. Here we measured cytokine expression in human <it>ex vivo </it>TH2 cells upon activation with anti-CD3/28 and challenge with an NKG2A-specific agonist.</p> <p>Methods</p> <p>TH2 cells were purified from healthy volunteers and activated with anti-CD3/28 in the presence and absence of NKG2A-specific agonist. IL-4 was used as a marker of TH2 effector function and measured by flow cytometry.</p> <p>Results</p> <p>Activation of TH2 cells increased NKG2A positivity from (Mean ± SE) 7.3 ± 2.4% to 13.7 ± 3.8%; (p = 0.03). The presence of NKG2A agonist did not significantly alter NKG2A expression, however, the percentage of activated TH2 cells expressing intracellular IL-4 decreased from 25.5 ± 6.8% to 9.3 ± 4.8% (p = 0.001).</p> <p>Conclusion</p> <p>We show that signalling through NKG2A suppresses TH2 effector function. This may provide a means to modulate Th1/Th2 balance in diseases where Th2 cytokines predominate.</p> |
| first_indexed | 2024-12-11T22:15:34Z |
| format | Article |
| id | doaj.art-a390e6aeaef6461dbf95e95276355dbc |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-12-11T22:15:34Z |
| publishDate | 2007-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-a390e6aeaef6461dbf95e95276355dbc2022-12-22T00:48:37ZengBMCBMC Pulmonary Medicine1471-24662007-10-01711410.1186/1471-2466-7-14NKG2A inhibits TH2 cell effector function <it>in vitro</it>Mojgani BaharFreishtat Robert JNazemzadeh MaryamNagaraju KanneboyinaHoffman Eric P<p>Abstract</p> <p>Background</p> <p>We previously reported that NKG2A, a key inhibitory ligand for HLA-E, is expressed on activated TH2 but not TH1 cells. Here we measured cytokine expression in human <it>ex vivo </it>TH2 cells upon activation with anti-CD3/28 and challenge with an NKG2A-specific agonist.</p> <p>Methods</p> <p>TH2 cells were purified from healthy volunteers and activated with anti-CD3/28 in the presence and absence of NKG2A-specific agonist. IL-4 was used as a marker of TH2 effector function and measured by flow cytometry.</p> <p>Results</p> <p>Activation of TH2 cells increased NKG2A positivity from (Mean ± SE) 7.3 ± 2.4% to 13.7 ± 3.8%; (p = 0.03). The presence of NKG2A agonist did not significantly alter NKG2A expression, however, the percentage of activated TH2 cells expressing intracellular IL-4 decreased from 25.5 ± 6.8% to 9.3 ± 4.8% (p = 0.001).</p> <p>Conclusion</p> <p>We show that signalling through NKG2A suppresses TH2 effector function. This may provide a means to modulate Th1/Th2 balance in diseases where Th2 cytokines predominate.</p>http://www.biomedcentral.com/1471-2466/7/14 |
| spellingShingle | Mojgani Bahar Freishtat Robert J Nazemzadeh Maryam Nagaraju Kanneboyina Hoffman Eric P NKG2A inhibits TH2 cell effector function <it>in vitro</it> BMC Pulmonary Medicine |
| title | NKG2A inhibits TH2 cell effector function <it>in vitro</it> |
| title_full | NKG2A inhibits TH2 cell effector function <it>in vitro</it> |
| title_fullStr | NKG2A inhibits TH2 cell effector function <it>in vitro</it> |
| title_full_unstemmed | NKG2A inhibits TH2 cell effector function <it>in vitro</it> |
| title_short | NKG2A inhibits TH2 cell effector function <it>in vitro</it> |
| title_sort | nkg2a inhibits th2 cell effector function it in vitro it |
| url | http://www.biomedcentral.com/1471-2466/7/14 |
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