Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938
An experimental design based on Response Surface Methodology (RSM) was used for the formulation of a growth medium based on sugar beet and wheat processing coproducts adapted to the cultivation of Lactobacillus reuteri (L. reuteri) DSM17938. The strain was cultivated on 30 different media varying by...
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MDPI AG
2017-07-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/3/3/32 |
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| author | Julien Couvreur Andreia R. S. Teixeira Florent Allais Henry-Eric Spinnler Claire Saulou-Bérion Tiphaine Clément |
| author_facet | Julien Couvreur Andreia R. S. Teixeira Florent Allais Henry-Eric Spinnler Claire Saulou-Bérion Tiphaine Clément |
| author_sort | Julien Couvreur |
| collection | DOAJ |
| description | An experimental design based on Response Surface Methodology (RSM) was used for the formulation of a growth medium based on sugar beet and wheat processing coproducts adapted to the cultivation of Lactobacillus reuteri (L. reuteri) DSM17938. The strain was cultivated on 30 different media varying by the proportions of sugar beet and wheat processing coproducts, and the concentration of yeast extract, tween 80 and vitamin B12. The media were used in a two-step process consisting of L. reuteri cultivation followed by the bioconversion of glycerol into 3-hydroxypropionic acid by resting cells. The efficiency of the formulations was evaluated according to the maximal optical density at the end of the growth phase (ΔOD620nm) and the ability of the resting cells to convert glycerol into 3-hydroxypropionic acid, a platform molecule of interest for the plastic industry. De Man, Rogosa, and Sharpe medium (MRS), commonly used for the cultivation of lactic bacteria, was used as the control medium. The optimized formulation allowed increasing the 3-HP production. |
| first_indexed | 2024-12-13T06:56:30Z |
| format | Article |
| id | doaj.art-a3919d134c1a45d2ab0a0f3a3761bd66 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-12-13T06:56:30Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-a3919d134c1a45d2ab0a0f3a3761bd662022-12-21T23:56:02ZengMDPI AGFermentation2311-56372017-07-01333210.3390/fermentation3030032fermentation3030032Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938Julien Couvreur0Andreia R. S. Teixeira1Florent Allais2Henry-Eric Spinnler3Claire Saulou-Bérion4Tiphaine Clément5Chaire Agro Biotechnologies Industrielles (ABI)-AgroParisTech, 3 rue des Rouges Terres, F-51110 Pomacle, FranceChaire Agro Biotechnologies Industrielles (ABI)-AgroParisTech, 3 rue des Rouges Terres, F-51110 Pomacle, FranceChaire Agro Biotechnologies Industrielles (ABI)-AgroParisTech, 3 rue des Rouges Terres, F-51110 Pomacle, FranceUMR 782 GMPA, AgroParisTech, Institut National de la Recherche Agronomique, Université Paris-Saclay, F-78850 Thiverval-Grignon, FranceUMR 782 GMPA, AgroParisTech, Institut National de la Recherche Agronomique, Université Paris-Saclay, F-78850 Thiverval-Grignon, FranceChaire Agro Biotechnologies Industrielles (ABI)-AgroParisTech, 3 rue des Rouges Terres, F-51110 Pomacle, FranceAn experimental design based on Response Surface Methodology (RSM) was used for the formulation of a growth medium based on sugar beet and wheat processing coproducts adapted to the cultivation of Lactobacillus reuteri (L. reuteri) DSM17938. The strain was cultivated on 30 different media varying by the proportions of sugar beet and wheat processing coproducts, and the concentration of yeast extract, tween 80 and vitamin B12. The media were used in a two-step process consisting of L. reuteri cultivation followed by the bioconversion of glycerol into 3-hydroxypropionic acid by resting cells. The efficiency of the formulations was evaluated according to the maximal optical density at the end of the growth phase (ΔOD620nm) and the ability of the resting cells to convert glycerol into 3-hydroxypropionic acid, a platform molecule of interest for the plastic industry. De Man, Rogosa, and Sharpe medium (MRS), commonly used for the cultivation of lactic bacteria, was used as the control medium. The optimized formulation allowed increasing the 3-HP production.https://www.mdpi.com/2311-5637/3/3/32Lactobacillus reuteriagroindustrial coproductsglycerol bioconversion3-hydroxypropionic acid |
| spellingShingle | Julien Couvreur Andreia R. S. Teixeira Florent Allais Henry-Eric Spinnler Claire Saulou-Bérion Tiphaine Clément Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938 Fermentation Lactobacillus reuteri agroindustrial coproducts glycerol bioconversion 3-hydroxypropionic acid |
| title | Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938 |
| title_full | Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938 |
| title_fullStr | Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938 |
| title_full_unstemmed | Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938 |
| title_short | Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938 |
| title_sort | wheat and sugar beet coproducts for the bioproduction of 3 hydroxypropionic acid by lactobacillus reuteri dsm17938 |
| topic | Lactobacillus reuteri agroindustrial coproducts glycerol bioconversion 3-hydroxypropionic acid |
| url | https://www.mdpi.com/2311-5637/3/3/32 |
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