Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model
Objective: To evaluate the potential effect of human Wharton’s jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×104/mL, 5×105/mL, 5×106/mL) were administered to rats on day 1 and day 8 after being induced...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=8;spage=343;epage=350;aulast=Widowati |
| _version_ | 1798037396832387072 |
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| author | Wahyu Widowati Teresa Liliana Wargasetia Fanny Rahardja Rimonta F Gunanegara Didik Priyandoko Marisca Evalina Gondokesumo Ervi Afifah Cahyaning Riski Wijayanti Rizal Rizal |
| author_facet | Wahyu Widowati Teresa Liliana Wargasetia Fanny Rahardja Rimonta F Gunanegara Didik Priyandoko Marisca Evalina Gondokesumo Ervi Afifah Cahyaning Riski Wijayanti Rizal Rizal |
| author_sort | Wahyu Widowati |
| collection | DOAJ |
| description | Objective: To evaluate the potential effect of human Wharton’s jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats.
Methods: The hWJMSCs (5×104/mL, 5×105/mL, 5×106/mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL- 18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry.
Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues.
Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome. |
| first_indexed | 2024-04-11T21:26:01Z |
| format | Article |
| id | doaj.art-a391bb4c47284ff8bc34e5366551c191 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-04-11T21:26:01Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-a391bb4c47284ff8bc34e5366551c1912022-12-22T04:02:24ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222022-01-0112834335010.4103/2221-1691.350182Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat modelWahyu WidowatiTeresa Liliana WargasetiaFanny RahardjaRimonta F GunanegaraDidik PriyandokoMarisca Evalina GondokesumoErvi AfifahCahyaning Riski WijayantiRizal RizalObjective: To evaluate the potential effect of human Wharton’s jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×104/mL, 5×105/mL, 5×106/mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL- 18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry. Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues. Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome.http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=8;spage=343;epage=350;aulast=Widowatihuman wharton’s jelly mesenchymal stem cells; acute respiratory distress syndrome; tnf-α; il-18; nf-κb; inflammatory marker |
| spellingShingle | Wahyu Widowati Teresa Liliana Wargasetia Fanny Rahardja Rimonta F Gunanegara Didik Priyandoko Marisca Evalina Gondokesumo Ervi Afifah Cahyaning Riski Wijayanti Rizal Rizal Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model Asian Pacific Journal of Tropical Biomedicine human wharton’s jelly mesenchymal stem cells; acute respiratory distress syndrome; tnf-α; il-18; nf-κb; inflammatory marker |
| title | Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model |
| title_full | Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model |
| title_fullStr | Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model |
| title_full_unstemmed | Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model |
| title_short | Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model |
| title_sort | human wharton s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model |
| topic | human wharton’s jelly mesenchymal stem cells; acute respiratory distress syndrome; tnf-α; il-18; nf-κb; inflammatory marker |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=8;spage=343;epage=350;aulast=Widowati |
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