Fast Screening of Biomembrane-Permeable Compounds in Herbal Medicines Using Bubble-Generating Magnetic Liposomes Coupled with LC–MS
A novel strategy based on the use of bionic membrane camouflaged magnetic particles and LC–MS was developed to quickly screen the biomembrane-permeable compounds in herbal medicines. The bionic membrane was constructed by bubble-generating magnetic liposomes loaded with NH<sub>4</sub>HCO...
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/6/1742 |
| _version_ | 1797540641646837760 |
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| author | Xiaoting Gu Dongwu Wang Xin Wang Youping Liu Xin Di |
| author_facet | Xiaoting Gu Dongwu Wang Xin Wang Youping Liu Xin Di |
| author_sort | Xiaoting Gu |
| collection | DOAJ |
| description | A novel strategy based on the use of bionic membrane camouflaged magnetic particles and LC–MS was developed to quickly screen the biomembrane-permeable compounds in herbal medicines. The bionic membrane was constructed by bubble-generating magnetic liposomes loaded with NH<sub>4</sub>HCO<sub>3</sub> (BMLs). The lipid bilayer structure of the liposomes enabled BMLs to capture biomembrane-permeable compounds from a herbal extract. The BMLs carrying the compounds were then separated from the extract by a magnetic field. Upon heat treatment, NH<sub>4</sub>HCO<sub>3</sub> rapidly decomposed to form CO<sub>2</sub> bubbles within the liposomal bilayer, and the captured compounds were released from BMLs and analyzed by LC–MS. Jinlingzi San (JLZS), which contains various natural ingredients, was chosen to assess the feasibility of the proposed method. As a result, nine potential permeable compounds captured by BMLs were identified for the first time. Moreover, an in vivo animal study found that most of the compounds screened out by the proposed method were absorbed into the blood. The study provides a powerful tool for rapid and simultaneous prediction of multiple biomembrane-permeable components. |
| first_indexed | 2024-03-10T13:04:03Z |
| format | Article |
| id | doaj.art-a394860960b44ad8844186a0f98f88d3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:04:03Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-a394860960b44ad8844186a0f98f88d32023-11-21T11:17:40ZengMDPI AGMolecules1420-30492021-03-01266174210.3390/molecules26061742Fast Screening of Biomembrane-Permeable Compounds in Herbal Medicines Using Bubble-Generating Magnetic Liposomes Coupled with LC–MSXiaoting Gu0Dongwu Wang1Xin Wang2Youping Liu3Xin Di4Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, ChinaLaboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, ChinaLaboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, ChinaLaboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, ChinaLaboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, ChinaA novel strategy based on the use of bionic membrane camouflaged magnetic particles and LC–MS was developed to quickly screen the biomembrane-permeable compounds in herbal medicines. The bionic membrane was constructed by bubble-generating magnetic liposomes loaded with NH<sub>4</sub>HCO<sub>3</sub> (BMLs). The lipid bilayer structure of the liposomes enabled BMLs to capture biomembrane-permeable compounds from a herbal extract. The BMLs carrying the compounds were then separated from the extract by a magnetic field. Upon heat treatment, NH<sub>4</sub>HCO<sub>3</sub> rapidly decomposed to form CO<sub>2</sub> bubbles within the liposomal bilayer, and the captured compounds were released from BMLs and analyzed by LC–MS. Jinlingzi San (JLZS), which contains various natural ingredients, was chosen to assess the feasibility of the proposed method. As a result, nine potential permeable compounds captured by BMLs were identified for the first time. Moreover, an in vivo animal study found that most of the compounds screened out by the proposed method were absorbed into the blood. The study provides a powerful tool for rapid and simultaneous prediction of multiple biomembrane-permeable components.https://www.mdpi.com/1420-3049/26/6/1742bubble-generating magnetic liposomesbionic membranepermeable compoundsherbal medicinesLC–MS |
| spellingShingle | Xiaoting Gu Dongwu Wang Xin Wang Youping Liu Xin Di Fast Screening of Biomembrane-Permeable Compounds in Herbal Medicines Using Bubble-Generating Magnetic Liposomes Coupled with LC–MS Molecules bubble-generating magnetic liposomes bionic membrane permeable compounds herbal medicines LC–MS |
| title | Fast Screening of Biomembrane-Permeable Compounds in Herbal Medicines Using Bubble-Generating Magnetic Liposomes Coupled with LC–MS |
| title_full | Fast Screening of Biomembrane-Permeable Compounds in Herbal Medicines Using Bubble-Generating Magnetic Liposomes Coupled with LC–MS |
| title_fullStr | Fast Screening of Biomembrane-Permeable Compounds in Herbal Medicines Using Bubble-Generating Magnetic Liposomes Coupled with LC–MS |
| title_full_unstemmed | Fast Screening of Biomembrane-Permeable Compounds in Herbal Medicines Using Bubble-Generating Magnetic Liposomes Coupled with LC–MS |
| title_short | Fast Screening of Biomembrane-Permeable Compounds in Herbal Medicines Using Bubble-Generating Magnetic Liposomes Coupled with LC–MS |
| title_sort | fast screening of biomembrane permeable compounds in herbal medicines using bubble generating magnetic liposomes coupled with lc ms |
| topic | bubble-generating magnetic liposomes bionic membrane permeable compounds herbal medicines LC–MS |
| url | https://www.mdpi.com/1420-3049/26/6/1742 |
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