Broad Environmental Tolerance for a <i>Salicola</i> Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USA
Phages greatly influence the ecology and evolution of their bacterial hosts; however, compared to hosts, a relatively low number of phages, especially halophilic phages, have been studied. This study describes a comparative investigation of physicochemical tolerance between a strain of the halophili...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-04-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/7/4/106 |
| _version_ | 1811312553896181760 |
|---|---|
| author | Meghan L. Rodela Shereen Sabet Allison Peterson Jesse G. Dillon |
| author_facet | Meghan L. Rodela Shereen Sabet Allison Peterson Jesse G. Dillon |
| author_sort | Meghan L. Rodela |
| collection | DOAJ |
| description | Phages greatly influence the ecology and evolution of their bacterial hosts; however, compared to hosts, a relatively low number of phages, especially halophilic phages, have been studied. This study describes a comparative investigation of physicochemical tolerance between a strain of the halophilic bacterium, <i>Salicola</i>, isolated from the Cargill Saltworks (Newark, CA, USA) and its associated phage. The host grew in media between pH 6–8.5, had a salinity growth optimum of 20% total salts (ranging from 10%–30%) and an upper temperature growth limit of 48 °C. The host utilized 61 of 190 substrates tested using BIOLOG Phenotype MicroArrays. The CGφ29 phage, one of only four reported <i>Salicola</i> phages, is a DNA virus of the <i>Siphoviridae</i> family. Overall, the phage tolerated a broader range of environmental conditions than its host (salinity 0–30% total salts; pH 3–9; upper thermal limit 80 °C) and is the most thermotolerant halophilic phage ever reported. This study is the most comprehensive investigation to date of a <i>Salicola</i> host–phage pair and provides novel insights into extreme environmental tolerances among bacteriophages. |
| first_indexed | 2024-04-13T10:39:30Z |
| format | Article |
| id | doaj.art-a396c73216794dd0aead9dfa8f9ffe8b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-13T10:39:30Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a396c73216794dd0aead9dfa8f9ffe8b2022-12-22T02:49:58ZengMDPI AGMicroorganisms2076-26072019-04-017410610.3390/microorganisms7040106microorganisms7040106Broad Environmental Tolerance for a <i>Salicola</i> Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USAMeghan L. Rodela0Shereen Sabet1Allison Peterson2Jesse G. Dillon3Department of Biological Sciences, California State University, Long Beach, CA 90840, USADepartment of Biological Sciences, California State University, Long Beach, CA 90840, USADepartment of Biological Sciences, California State University, Long Beach, CA 90840, USADepartment of Biological Sciences, California State University, Long Beach, CA 90840, USAPhages greatly influence the ecology and evolution of their bacterial hosts; however, compared to hosts, a relatively low number of phages, especially halophilic phages, have been studied. This study describes a comparative investigation of physicochemical tolerance between a strain of the halophilic bacterium, <i>Salicola</i>, isolated from the Cargill Saltworks (Newark, CA, USA) and its associated phage. The host grew in media between pH 6–8.5, had a salinity growth optimum of 20% total salts (ranging from 10%–30%) and an upper temperature growth limit of 48 °C. The host utilized 61 of 190 substrates tested using BIOLOG Phenotype MicroArrays. The CGφ29 phage, one of only four reported <i>Salicola</i> phages, is a DNA virus of the <i>Siphoviridae</i> family. Overall, the phage tolerated a broader range of environmental conditions than its host (salinity 0–30% total salts; pH 3–9; upper thermal limit 80 °C) and is the most thermotolerant halophilic phage ever reported. This study is the most comprehensive investigation to date of a <i>Salicola</i> host–phage pair and provides novel insights into extreme environmental tolerances among bacteriophages.https://www.mdpi.com/2076-2607/7/4/106<i>Salicola</i>phagehalophilesalternthermotolerant |
| spellingShingle | Meghan L. Rodela Shereen Sabet Allison Peterson Jesse G. Dillon Broad Environmental Tolerance for a <i>Salicola</i> Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USA Microorganisms <i>Salicola</i> phage halophile saltern thermotolerant |
| title | Broad Environmental Tolerance for a <i>Salicola</i> Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USA |
| title_full | Broad Environmental Tolerance for a <i>Salicola</i> Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USA |
| title_fullStr | Broad Environmental Tolerance for a <i>Salicola</i> Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USA |
| title_full_unstemmed | Broad Environmental Tolerance for a <i>Salicola</i> Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USA |
| title_short | Broad Environmental Tolerance for a <i>Salicola</i> Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USA |
| title_sort | broad environmental tolerance for a i salicola i host phage pair isolated from the cargill solar saltworks newark ca usa |
| topic | <i>Salicola</i> phage halophile saltern thermotolerant |
| url | https://www.mdpi.com/2076-2607/7/4/106 |
| work_keys_str_mv | AT meghanlrodela broadenvironmentaltoleranceforaisalicolaihostphagepairisolatedfromthecargillsolarsaltworksnewarkcausa AT shereensabet broadenvironmentaltoleranceforaisalicolaihostphagepairisolatedfromthecargillsolarsaltworksnewarkcausa AT allisonpeterson broadenvironmentaltoleranceforaisalicolaihostphagepairisolatedfromthecargillsolarsaltworksnewarkcausa AT jessegdillon broadenvironmentaltoleranceforaisalicolaihostphagepairisolatedfromthecargillsolarsaltworksnewarkcausa |